Results 1 to 10 of about 1,193,059 (373)

Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration [PDF]

Journal of Visualized Experiments, 2014
Recombinant protein engineering has utilized Escherichia coli (E. coli) expression systems for nearly 4 decades, and today E. coli is still the most widely used host organism. The flexibility of the system allows for the addition of moieties such as a biotin tag (for streptavidin interactions) and larger functional proteins like green fluorescent ...
Aleesha M. McCormick, Natalie A. Jarmusik, Elizabeth J. Endrizzi, Nic D. Leipzig   +3 more
semanticscholar   +5 more sources

Ca2+ and cyclic AMP regulate phosphorylation of same two membrane-associated proteins specific to nerve tissue. [PDF]

Proceedings of the National Academy of Sciences, 1979
It was shown previously that addition of cyclic AMP (cAMP) to a synaptic membrane fraction incubated with [gamma-32P]ATP stimulated the phosphorylation of two proteins, designated proteins Ia and Ib, found only in nerve tissue. Addition of Ca2+ plus veratridine to synaptosomes preincubated with 32Pi stimulated the phosphorylation of two proteins with ...
Werner Sieghart, J Forn, Paul Greengard
semanticscholar   +5 more sources

Aligned Protein–Polymer Composite Fibers Enhance Nerve Regeneration: A Potential Tissue‐Engineering Platform [PDF]

Advanced Functional Materials, 2007
AbstractSustained release of proteins from aligned polymeric fibers holds great potential in tissue‐engineering applications. These protein–polymer composite fibers possess high surface‐area‐to‐volume ratios for cell attachment, and can provide biochemical and topographic cues to enhance tissue regeneration.
Sing Yian Chew, Rongjuan Mi, Ahmet Höke, Kam W. Leong   +3 more
semanticscholar   +5 more sources

Cell-free therapy based on extracellular vesicles: a promising therapeutic strategy for peripheral nerve injury

Stem Cell Research & Therapy, 2023
Peripheral nerve injury (PNI) is one of the public health concerns that can result in a loss of sensory or motor function in the areas in which injured and non-injured nerves come together.
Mojdeh Salehi Namini, Fatemeh Daneshimehr, Nima Beheshtizadeh, Vahid Mansouri, Jafar Ai, Hossein Kargar Jahromi, Somayeh Ebrahimi-Barough   +6 more
doaj   +2 more sources

A Bioinspired Self‐Healing Conductive Hydrogel Promoting Peripheral Nerve Regeneration

Advanced Science, 2023
The development of self‐healing conductive hydrogels is critical in electroactive nerve tissue engineering. Typical conductive materials such as polypyrrole (PPy) are commonly used to fabricate artificial nerve conduits.
Hongyun Xuan, Shuyuan Wu, Yan Jin, Shuo Wei, Feng Xiong, Ye Xue, Biyun Li, Yumin Yang, Huihua Yuan   +8 more
doaj   +2 more sources

The injured sciatic nerve atlas (iSNAT), insights into the cellular and molecular basis of neural tissue degeneration and regeneration

eLife, 2022
Upon trauma, the adult murine peripheral nervous system (PNS) displays a remarkable degree of spontaneous anatomical and functional regeneration. To explore extrinsic mechanisms of neural repair, we carried out single-cell analysis of naïve mouse sciatic
Xiao-Feng Zhao, Lucas D Huffman, Hannah Hafner, Mitre Athaiya, Matthew C Finneran, Ashley L Kalinski, Rafi Kohen, Corey Flynn, Ryan Passino, Craig N Johnson, David Kohrman, Riki Kawaguchi, Lynda JS Yang, Jeffery L Twiss, Daniel H Geschwind, Gabriel Corfas, Roman J Giger   +16 more
doaj   +2 more sources

Refinement of Protein Extraction Protocols for Human Peripheral Nerve Tissue

ACS Omega
Our aim was to establish an effective method for protein extraction from freshly frozen human peripheral nerves, determine the minimum amount required for consistent protein extraction outcomes, and assess which method produced the highest number of protein identities.
Drifa Frostadottir, Charlotte Welinder, Raquel Perez, Lars B. Dahlin   +3 more
openalex   +5 more sources

Recent advances in the application of MXenes for neural tissue engineering and regeneration

Neural Regeneration Research, 2023
Transition metal carbides and nitrides (MXenes) are crystal nanomaterials with a number of surface functional groups such as fluorine, hydroxyl, and oxygen, which can be used as carriers for proteins and drugs.
Menghui Liao, Qingyue Cui, Yangnan Hu, Jiayue Xing, Danqi Wu, Shasha Zheng, Yu Zhao, Yafeng Yu, Jingwu Sun, Renjie Chai   +9 more
doaj   +2 more sources

Development of a Novel Tissue Targeted Nerve Growth Factor: Fibronectin Chimeric Protein as a Potential Therapeutic for Peripheral Nerve Regeneration [PDF]

, 2018
This project sought to develop a novel regenerative fusion protein that directly targets nerve-tissue through the addition of a specific nerve-tissue binding domain. Combining select domains of the extracellular matrix protein fibronectin with nerve growth factor, a singular potent regenerative stimulant was developed.
Mangapathiraju Tippana
openalex   +3 more sources

Matrilin-2, an extracellular adaptor protein, is needed for the regeneration of muscle, nerve and other tissues

Neural Regeneration Research, 2015
The extracellular matrix (ECM) performs essential functions in the differentiation, maintenance and remodeling of tissues during development and regeneration, and it undergoes dynamic changes during remodeling concomitant to alterations in the cell-ECM interactions.
IbolyaKiss, ÉvaKorpos, FerencDeák
openalex   +4 more sources