Expression, Isolation, and Purification of Soluble and Insoluble Biotinylated Proteins for Nerve Tissue Regeneration [PDF]
Journal of Visualized Experiments, 2014 Recombinant protein engineering has utilized Escherichia coli (E. coli) expression systems for nearly 4 decades, and today E. coli is still the most widely used host organism. The flexibility of the system allows for the addition of moieties such as a biotin tag (for streptavidin interactions) and larger functional proteins like green fluorescent ...
Natalie A. Jarmusik +3 more
semanticscholar +5 more sources
Ca2+ and cyclic AMP regulate phosphorylation of same two membrane-associated proteins specific to nerve tissue. [PDF]
Proceedings of the National Academy of Sciences, 1979 It was shown previously that addition of cyclic AMP (cAMP) to a synaptic membrane fraction incubated with [gamma-32P]ATP stimulated the phosphorylation of two proteins, designated proteins Ia and Ib, found only in nerve tissue. Addition of Ca2+ plus veratridine to synaptosomes preincubated with 32Pi stimulated the phosphorylation of two proteins with ...
Paul Greengard +2 more
semanticscholar +5 more sources
Aligned Protein–Polymer Composite Fibers Enhance Nerve Regeneration: A Potential Tissue‐Engineering Platform [PDF]
Advanced Functional Materials, 2007 AbstractSustained release of proteins from aligned polymeric fibers holds great potential in tissue‐engineering applications. These protein–polymer composite fibers possess high surface‐area‐to‐volume ratios for cell attachment, and can provide biochemical and topographic cues to enhance tissue regeneration.
Kam W. Leong +3 more
semanticscholar +5 more sources
Refinement of Protein Extraction Protocols for Human Peripheral Nerve Tissue. [PDF]
ACS OmegaFrostadottir D +3 more
europepmc +4 more sources
Immunohistochemical localization of nerve injury-induced protein-1 in mouse tissues [PDF]
Anatomy & Cell Biology, 2019 Nerve injury-induced protein (Ninjurin)-1 is a cell adhesion molecule that is upregulated in neurons and Schwann cells after transection injury in rats. In this study, we investigated the localization of Ninjurin-1 in various tissues, including the cerebrum, sciatic nerve, spleen, lung, stomach, ileum, colon, liver, pancreas, kidney, testis, and skin ...
Poornima Ekanayake +4 more
openaire +4 more sources
Development of a Novel Tissue Targeted Nerve Growth Factor: Fibronectin Chimeric Protein as a Potential Therapeutic for Peripheral Nerve Regeneration [PDF]
, 2018 This project sought to develop a novel regenerative fusion protein that directly targets nerve-tissue through the addition of a specific nerve-tissue binding domain. Combining select domains of the extracellular matrix protein fibronectin with nerve growth factor, a singular potent regenerative stimulant was developed.
Mangapathiraju Tippana
openaire +3 more sources
Matrilin-2, an extracellular adaptor protein, is needed for the regeneration of muscle, nerve and other tissues [PDF]
Neural Regeneration Research, 2015 The extracellular matrix (ECM) performs essential functions in the differentiation, maintenance and remodeling of tissues during development and regeneration, and it undergoes dynamic changes during remodeling concomitant to alterations in the cell-ECM interactions.
Eva Korpos, Ferenc Deák, Ibolya Kiss
openaire +5 more sources
Alternative exon 3 splicing of the human major protein zero gene in white blood cells and peripheral nerve tissue [PDF]
FEBS Letters, 1999 The major protein zero (MPZ) is involved in peripheral myelin folding. Using nested reverse transcription‐PCR, we amplified several fragments of MPZ mRNAs in white blood cells and in peripheral nerve tissue. Cloning of PCR products revealed the existence of three alternative splicing patterns: one resulted in the complete loss of exon 3 and two others ...
Philippe Latour +9 more
openaire +5 more sources
Nerve tissue protein S‐100 and neurone‐specific enolase concentrations in cerebrospinal fluid and blood during carotid endarterectomy [PDF]
Anaesthesia, 2000 Nerve tissue protein S‐100 and neurone‐specific enolase levels in serum were studied in 10 patients before, during and for 2 days after elective carotid endarterectomy performed under general anaesthesia and using a Javid Shunt. In six patients, simultaneous cerebrospinal fluid samples were also obtained.
N. J. Standfield +3 more
openaire +4 more sources
Exosome Therapy in Stress Urinary Incontinence: A Comprehensive Literature Review. [PDF]
BiomedicinesNasrollahzadeh Saravi M +5 more
europepmc +1 more source