Results 271 to 280 of about 97,206 (312)

Nested polymerase chain reaction for detection of Legionella pneumophila in water

Research in Microbiology, 1994
A highly sensitive nested polymerase chain reaction (PCR) method was evaluated for detection of Legionella pneumophila in water. Two sets of primers homologous to the coding region of the L. pneumophila macrophage infectivity potentiator (mip) gene were used. Even when starting from minute amounts of L.
V, Catalan, C, Moreno, M A, Dasi, C, Muñoz, D, Apraiz   +4 more
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Nested polymerase chain reaction in the diagnosis of congenital cytomegalovirus infection

The Indian Journal of Pediatrics, 2001
Human cytomegalovirus infection is highly prevalent in Indian population. It is the commonest congenitally acquired infection causing various anomalies. The diagnosis of infection in neonates is difficult as IgM may not be detected in all cases. The polymerase chain reaction is reported as alternative and better option in these patients. However, there
R, Sivakumar, N, Singh, S, Singh
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Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction

Veterinary Parasitology, 2001
We describe a nested polymerase chain reaction (PCR) for the detection of Babesia equi in equine infected erythrocytes using oligonucleotides designed on the published sequence of a B. equi merozoite antigen gene (ema-1). A 102bp DNA fragment is specifically amplified from B. equi but not from Babesia caballi, Babesia bovis or Babesia bigemina DNA.
T B, Nicolaiewsky, M F, Richter, V R, Lunge, C W, Cunha, O, Delagostin, N, Ikuta, A S, Fonseca, S S, da Silva, L S, Ozaki   +8 more
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Detection of feline immunodeficiency virus by a nested polymerase chain reaction

Journal of Virological Methods, 1992
A specific and sensitive polymerase chain reaction (PCR) procedure for the detection of feline immunodeficiency virus (FIV) in peripheral blood mononuclear cells (PBMC) was developed. PBMC from both blood samples and cultures were digested by proteinase K in a lysis buffer, and after heat inactivation of the proteinase, the resultant material was used ...
E, Rimstad, K, Ueland
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Quantitation of polyomavirus DNA by a competitive nested polymerase chain reaction

Journal of Virological Methods, 2000
A new method to quantitate small amounts of DNA in clinical specimens is described. The method, a nested competitive polymerase chain reaction (ncPCR), is able to quantitate between 10 and 10(6) copies per tube of polyomavirus DNA and shows good reproducibility when clinical samples are analysed. Throughout the whole procedure, an internal standard (IS)
C. G. Fedele, A. Avellon, CIARDI, Maria Rosa, DELIA, Salvatore, A. Tenorio   +4 more
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Competitive nested polymerase chain reaction for quantification of humanMDR1 gene expression

Journal of Cancer Research and Clinical Oncology, 1994
Tumor cell resistance to cytotoxic drugs is considered one of the major obstacles to successful chemotherapy. Multidrug resistance (MDR) describes the simultaneous expression of cellular resistance to a wide range of structurally and functionally unrelated drugs.
F, Grünebach, E U, Griese, K, Schumacher   +2 more
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[Nested coamplification polymerase chain reaction].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics, 2002
To develop a simple and reliable method for intensifying the hybridization signals of gene chips.The authors added EDTA and another FAM-labeled probe to the normal PCR products, denatured the mixture by heat, and then let the mixture hybridize with the fastened probes on the chip.With the use of EDTA and another FAM-labeled probe, the hybridization ...
Qingjie, Xia, Sizhong, Zhang, Yuan, Yang, Jun, Wang, Xiujie, Wang, Yongxin, Ma, Yan, Sun   +6 more
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Reliability of nested polymerase chain reaction in the diagnosis of bacterial endophthalmitis

American Journal of Ophthalmology, 2002
To test the utility of DNA Taq polymerase (Taq) treated with 8-methoxypsoralen with ultraviolet irradiation and Taq treated with restriction endonuclease in a nested polymerase chain reaction test for the diagnosis of bacterial endophthalmitis.Prospective, comparative study.
Savitri, Sharma, Debashish, Das, Raj, Anand, Taraprasad, Das, Chitra, Kannabiran   +4 more
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Detection of feline herpesvirus 1 DNA by the nested polymerase chain reaction

Veterinary Microbiology, 1996
The thymidine kinase region of feline herpesvirus 1 (FHV 1) genome in ocular/nasal swabs from cats with clinical manifestations of upper respiratory disease was amplified by nested polymerase chain reaction (nested PCR). Two primer pairs were prepared for nested PCR. FHV 1 DNA in ocular/nasal swabs was extracted using instaGene-DNA purification matrix.
M, Hara, M, Fukuyama, Y, Suzuki, S, Kisikawa, T, Ikeda, A, Kiuchi, K, Tabuchi   +6 more
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Nested polymerase chain reaction for detection of Mycobacterium tuberculosis in clinical samples

Journal of Clinical Microbiology, 1993
The nested polymerase chain reaction technique was compared with the conventional smear and culture methods for detection of Mycobacterium tuberculosis. The nested polymerase chain reaction used in this study showed excellent specificity, sensitivity, and agreement with the conventional methods for 417 clinical samples, indicating a contribution to the
Y, Miyazaki, H, Koga, S, Kohno, M, Kaku
openaire   +2 more sources