Results 41 to 50 of about 75,373 (266)
Microbiology Spectrum, 2023 Neutralizing antibodies (nAbs) are considered a valuable marker for measuring humoral immunity against SARS-CoV-2. However, live-virus neutralization tests (NTs) require high-biosafety-level laboratories and are time-consuming. Therefore, surrogate virus
Marianne Graninger +14 more
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Using cell culture in evaluation of Clostridium perfringens type A vaccines [PDF]
Veterinary Medical Journal - Giza, 2014 Cell culture assays are possible alternatives to replace in-vivo neutralization tests currently required for potency testing of clostridial vaccines. So, the objective of this work was to titrate C. perfringens type A alpha vaccine in Vero (African Green
Khodeir H, Sayed L, Fathia S
doaj +1 more source
Viruses, 2023 The henipaviruses, Nipah virus (NiV), and Hendra virus (HeV) can cause fatal diseases in humans and animals, whereas Cedar virus is a nonpathogenic henipavirus.
Moushimi Amaya +12 more
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Testing the neutral hypothesis of phenotypic evolution [PDF]
Proceedings of the National Academy of Sciences, 2016 Significance Despite the universal recognition that adaptation by Darwinian selection can shape phenotypic variations within and between species, it remains unknown whether most phenotypic variations observed have adaptive values, in part because addressing this question requires examining a large, random set of traits while past ...
Wei-Chin Ho +2 more
openaire +2 more sources
Pediatric Blood &Cancer, EarlyView.ABSTRACT Immune effector cell‐associated hemophagocytic lymphohistiocytosis‐like syndrome (IEC‐HS) is a life‐threatening hyperinflammatory toxicity distinct from cytokine release syndrome (CRS) and neurotoxicity following chimeric antigen receptor T‐cell (CAR‐T) therapy. In a single‐institution retrospective cohort of pediatric and young adult patients
Thomas J. Galletta +6 more
wiley +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Standardized two-step testing of antibody activity in COVID-19 convalescent plasma
iScience, 2022 Summary: The COVID-19 pandemic revealed an urgent need for rapid profiling of neutralizing antibody responses and development of antibody therapeutics. The current Food and Drug Administration-approved serological tests do not measure antibody-mediated ...
Pavlo Gilchuk +21 more
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Vero microcultures for adenovirus neutralization tests [PDF]
Journal of Clinical Microbiology, 1978 A microculture neutralization test is described for measuring specific antibody levels to the 35 human adenovirus serotypes in Vero cells. The test is read at 5 days by macroscopic observation after staining the uninfected cells with crystal violet.
J C, Hierholzer, P G, Bingham
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Structural insights and therapeutic targets in Acinetobacter baumannii capsule biosynthesis
FEBS Letters, EarlyView.Hypervirulent KL49 A. baumannii's capsular polysaccharide contains the nonulosonic acid 8‐epi‐Leg5,7Ac2, synthesized by epimerization via ElaA, ElaB, and ElaC. Crystal structures of ElaA, ElaB, and ElaC reveal their role in CMP‐Leg5,7Ac2 synthesis and regioselective C8 epimerization.
Woo Cheol Lee +7 more
wiley +1 more source