Results 41 to 50 of about 942,883 (343)

Suppressing gain-of-function proteins via CRISPR/Cas9 system in SCA1 cells

Scientific Reports, 2022
SCAs are autosomal dominant neurodegenerative disorders caused by a gain-of-function protein with toxic activities, containing an expanded polyQ tract in the coding region.
Mariangela Pappadà, Ottavia Bonuccelli, Mattia Buratto, Riccardo Fontana, Mariaconcetta Sicurella, Anna Caproni, Silvia Fuselli, Andrea Benazzo, Roberto Bertorelli, Veronica De Sanctis, Paolo Cavallerio, Valentina Simioni, Valeria Tugnoli, Francesca Salvatori, Peggy Marconi   +14 more
doaj   +1 more source

Decreased exosome-delivered miR-486-5p is responsible for the peritoneal metastasis of gastric cancer cells by promoting EMT progress

World Journal of Surgical Oncology, 2021
Background The present study aims to investigate the preliminary mechanism underlying the peritoneal metastasis of gastric cancer cells. Methods Exosomes from GC9811 cells (Con-Exo) and from GC9811-P cells (PM-Exo) were extracted by ultracentrifugation ...
Xian-Ming Lin, Zhi-Jiang Wang, Yu-Xiao Lin, Hao Chen   +3 more
doaj   +1 more source

Age-related changes in polycomb gene regulation disrupt lineage fidelity in intestinal stem cells

eLife, 2021
Tissue homeostasis requires long-term lineage fidelity of somatic stem cells. Whether and how age-related changes in somatic stem cells impact the faithful execution of lineage decisions remains largely unknown.
Helen M Tauc, Imilce A Rodriguez-Fernandez, Jason A Hackney, Michal Pawlak, Tal Ronnen Oron, Jerome Korzelius, Hagar F Moussa, Subhra Chaudhuri, Zora Modrusan, Bruce A Edgar, Heinrich Jasper   +10 more
doaj   +1 more source

Base calling for high-throughput short-read sequencing: dynamic programming solutions [PDF]

, 2013
Shreepriya Das and Haris Vikalo are with the Electrical and Computer Engineering Department, The University of Texas at Austin, Austin, Texas 78712, USABackground: Next-generation DNA sequencing platforms are capable of generating millions of reads in ...
Das, Shreepriya, Vikalo, Haris
core   +3 more sources

Bioinformatics and Next- generation Sequencing [PDF]

Klinicka onkologie, 2015
Next-generation sequencing technologies are currently well-established in the research field and progressively find their way towards clinical applications. Sequencers produce vast amounts of data and therefore bioinformatics methods are needed for processing.
Bořivoj Vojtěšek, Adam Krejci, Petr Müller   +2 more
openaire   +2 more sources

Next generation sequencing [PDF]

, 2013
Informacije pohranjene u DNA sekvenci sadrži vrijedne podatke o tom organizmu, i znajući sekvencu cijelog genoma, u mogućnosti smo razumjeti procese koji se odvijaju u tom biću.
Vučenović, Dunja
core   +2 more sources

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

occumb: An R package for site occupancy modeling of eDNA metabarcoding data

Population Ecology, EarlyView.
This study introduces a new R package, occumb, for the convenient application of site occupancy modeling using environmental DNA (eDNA) metabarcoding data. We outline a data analysis workflow, including data setup, model fitting, model assessment, and comparison of potential study settings based on model predictions, all of which can be performed using
Keiichi Fukaya, Yuta Hasebe
wiley   +1 more source

High Detection Frequency of Vaccine-Associated Polioviruses and Non-Polio Enteroviruses in the Stools of Asymptomatic Infants from the Free State Province, South Africa

Microorganisms
Enterovirus (EV) infections are widespread and associated with a range of clinical conditions, from encephalitis to meningitis, gastroenteritis, and acute flaccid paralysis.
Milton T. Mogotsi, Ayodeji E. Ogunbayo, Hester G. O’Neill, Martin M. Nyaga   +3 more
doaj   +1 more source

Molecular Characterisation of a Rare Reassortant Porcine-Like G5P[6] Rotavirus Strain Detected in an Unvaccinated Child in Kasama, Zambia

Pathogens, 2020
A human-porcine reassortant strain, RVA/Human-wt/ZMB/UFS-NGS-MRC-DPRU4723/2014/G5P[6], was identified in a sample collected in 2014 from an unvaccinated 12 month old male hospitalised for gastroenteritis in Zambia.
Wairimu M. Maringa, Peter N. Mwangi, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Ina Peenze, Mathew D. Esona, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga   +9 more
doaj   +1 more source