Results 11 to 20 of about 963,855 (274)
Next-generation sequencing demands next-generation phenotyping [PDF]
Human Mutation, 2012 Next-generation sequencing (NGS) is the most powerful diagnostic tool since the roentgenogram. NGS will facilitate diagnosis on a massive scale, allowing interrogation of all genes in a single assay. It has been suggested that NGS will decrease the need for phenotyping in general and medical geneticists in particular. We argue that NGS will shift focus
Hennekam, Raoul C. M. +1 more
openaire +3 more sources
Next-Generation Sequencing Technologies [PDF]
Cold Spring Harbor Perspectives in Medicine, 2018 Although DNA and RNA sequencing has a history spanning five decades, large-scale massively parallel sequencing, or next-generation sequencing (NGS), has only been commercially available for about 10 years. Nonetheless, the meteoric increase in sequencing throughput with NGS has dramatically changed our understanding of our genome and ourselves ...
McCombie, W Richard +2 more
openaire +4 more sources
Fluorescent amplification for next generation sequencing (FA-NGS) library preparation. [PDF]
, 2020 BACKGROUND:Next generation sequencing (NGS) has become a universal practice in modern molecular biology. As the throughput of sequencing experiments increases, the preparation of conventional multiplexed libraries becomes more labor intensive ...
Chiniquy, Jennifer +3 more
core +1 more source
Next-Generation Sequencing Strategies [PDF]
Cold Spring Harbor Perspectives in Medicine, 2018 More than a decade ago, the term "next-generation" sequencing was coined to describe what was, at the time, revolutionary new methods to sequence RNA and DNA at a faster pace and cheaper cost than could be performed by standard bench-top protocols. Since then, the field of DNA sequencing has evolved at a rapid pace, with new breakthroughs allowing ...
Shawn E, Levy, Braden E, Boone
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Scientific Reports, 2022 Gulosibacter molinativorax ON4T is the only known organism to produce molinate hydrolase (MolA), which catalyses the breakdown of the thiocarbamate herbicide into azepane-1-carboxylic acid (ACA) and ethanethiol.
A. R. Lopes +11 more
doaj +1 more source
Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) [PDF]
PeerJ, 2018 Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular ...
Weiping Shi +12 more
doaj +2 more sources
High-throughput Quant-iT PicoGreen assay using an automated liquid handling system
BioTechniques, 2019 Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat +3 more
doaj +1 more source
Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity
Cell Reports, 2023 Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi +19 more
doaj +1 more source
Pathogens, 2020 Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi +8 more
doaj +1 more source
Viruses, 2023 The G2P[4] genotype is among the rotavirus strains that circulate commonly in humans. Several countries have reported its immediate upsurge after the introduction of rotavirus vaccination, raising concern about sub-optimal vaccine effectiveness against ...
Peter N. Mwangi +10 more
doaj +1 more source