Results 21 to 30 of about 978,334 (297)
Transcriptomic studies reveal a key metabolic pathway contributing to a well-maintained photosynthetic system under drought stress in foxtail millet (Setaria italica L.) [PDF]
PeerJ, 2018 Drought stress is one of the most important abiotic factors limiting crop productivity. A better understanding of the effects of drought on millet (Setaria italica L.) production, a model crop for studying drought tolerance, and the underlying molecular ...
Weiping Shi +12 more
doaj +2 more sources
High-throughput Quant-iT PicoGreen assay using an automated liquid handling system
BioTechniques, 2019 Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat +3 more
doaj +1 more source
NGSNGS: next-generation simulator for next-generation sequencing data [PDF]
Bioinformatics, 2023 AbstractSummaryWith the rapid expansion of the capabilities of the DNA sequencers throughout the different sequencing generations, the quantity of generated data has likewise increased. This evolution has also led to new bioinformatical methods, for which in silico data have become crucial when verifying the accuracy of a model or the robustness of a ...
Rasmus Amund Henriksen +2 more
openaire +3 more sources
Next-generation sequencing [PDF]
Breast Cancer Research, 2009 Next-generation sequencing (also known as massively parallel sequencing) technologies are revolutionising our ability to characterise cancers at the genomic, transcriptomic and epigenetic levels. Cataloguing all mutations, copy number aberrations and somatic rearrangements in an entire cancer genome at base pair resolution can now be performed in a ...
openaire +2 more sources
Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity
Cell Reports, 2023 Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi +19 more
doaj +1 more source
A normalization technique for next generation sequencing experiments [PDF]
, 2010 Next generation sequencing (NGS) are these days one of the key technologies in biology. NGS' cost effectiveness and capability of finding the smallest variations in the genome makes them increasingly popular.
Andreas Mayr +4 more
core +3 more sources
Pathogens, 2020 Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi +8 more
doaj +1 more source
Viruses, 2023 The G2P[4] genotype is among the rotavirus strains that circulate commonly in humans. Several countries have reported its immediate upsurge after the introduction of rotavirus vaccination, raising concern about sub-optimal vaccine effectiveness against ...
Peter N. Mwangi +10 more
doaj +1 more source
A genome-wide survey of mutations in the Jurkat cell line
BMC Genomics, 2018 Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia +4 more
doaj +1 more source
Evaluation of experimental design and computational parameter choices affecting analyses of ChIP-seq and RNA-seq data in undomesticated poplar trees. [PDF]
, 2014 BackgroundOne of the great advantages of next generation sequencing is the ability to generate large genomic datasets for virtually all species, including non-model organisms.
Filkov, Vladimir +4 more
core +2 more sources