Results 41 to 50 of about 978,185 (157)

Single cell transcriptome analysis using next generation sequencing. [PDF]

, 2010
The heterogeneity of tissues, especially in cancer research, is a central issue in transcriptome analysis. In recent years, research has primarily focused on the development of methods for single cell analysis. Single cell analysis aims at gaining (novel)
Blattner, M.
core   +1 more source

Molecular Characterisation of a Rare Reassortant Porcine-Like G5P[6] Rotavirus Strain Detected in an Unvaccinated Child in Kasama, Zambia

Pathogens, 2020
A human-porcine reassortant strain, RVA/Human-wt/ZMB/UFS-NGS-MRC-DPRU4723/2014/G5P[6], was identified in a sample collected in 2014 from an unvaccinated 12 month old male hospitalised for gastroenteritis in Zambia.
Wairimu M. Maringa, Peter N. Mwangi, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Ina Peenze, Mathew D. Esona, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga   +9 more
doaj   +1 more source

A Pilot Study Comparing HPV-Positive and HPV-Negative Head and Neck Squamous Cell Carcinomas by Whole Exome Sequencing. [PDF]

, 2012
Background. Next-generation sequencing of cancers has identified important therapeutic targets and biomarkers. The goal of this pilot study was to compare the genetic changes in a human papillomavirus- (HPV-)positive and an HPV-negative head and neck ...
Barrett, John W, Basmaji, John, Boutros, Paul, Chan-Seng-Yue, Michelle, Dhaliwal, Sandeep, Dowthwaite, Samuel, Franklin, Jason H, Fung, Kevin, Koropatnick, James, Kwan, Keith, Lambin, Philippe, Mymryk, Joe S, Nichols, Anthony C, Palma, David A, Starmans, Maud HW, Szeto, Christopher CT, Todorovic, Biljana, Wehrli, Bret, Xu, Wei, Yoo, John   +19 more
core   +3 more sources

Unveiling a Shift in the Rotavirus Strains in Benin: Emergence of Reassortment Intergenogroup and Equine-like G3P[8] Strains in the Post-Vaccination Era

Viruses
While a global downward trend in rotavirus diarrhea cases has been observed following vaccine introduction, reassortment, genetic drift, and vaccine-escaping strains remain a concern, particularly in Sub-Saharan Africa.
Jijoho M. Agbla, Milton T. Mogotsi, Alban G. Zohoun, Nkosazana D. Shange, Annick Capochichi, Ayodeji E. Ogunbayo, Rolande Assogba, Shainey Khakha, Aristide Sossou, Hlengiwe Sondlane, Jason M. Mwenda, Mathew D. Esona, Martin M. Nyaga   +12 more
doaj   +1 more source

SARS-CoV-2 variants from COVID-19 positive cases in the Free State province, South Africa from July 2020 to December 2021

Frontiers in Virology, 2022
Since the COVID-19 outbreak emerged, SARS-CoV-2 has continuously evolved into variants with underlying mutations associated with increased transmissibility, potential escape from neutralizing antibodies, and disease severity.
Peter Mwangi, Javan Okendo, Javan Okendo, Milton Mogotsi, Ayodeji Ogunbayo, Olusesan Adelabu, Hlengiwe Sondlane, Makgotso Maotoana, Lutfiyya Mahomed, Molefi Daniel Morobadi, Molefi Daniel Morobadi, Sabeehah Vawda, Sabeehah Vawda, Anne von Gottberg, Anne von Gottberg, Jinal Bhiman, Jinal Bhiman, Houriiyah Tegally, Eduan Wilkinson, Jennifer Giandhari, Sureshnee Pillay, Yeshnee Naidoo, Upasana Ramphal, Tulio de Oliveira, Tulio de Oliveira, Tulio de Oliveira, Armand Bester, Armand Bester, Dominique Goedhals, Dominique Goedhals, Dominique Goedhals, Martin Nyaga   +31 more
doaj   +1 more source

Molecular insights into Solanum sisymbriifolium’s resistance against Globodera pallida via RNA-seq

BMC Plant Biology
Background The presence of potato cyst nematodes (PCN) causes a significant risk to potato crops globally, leading to reduced yields and economic losses. While the plant Solanum sisymbriifolium is known for its resistance to PCN and can be used as a trap
Raquel Varandas, Cristina Barroso, Isabel Luci Conceição, Conceição Egas   +3 more
doaj   +1 more source

Longitudinal analysis of the enteric virome in paediatric subjects from the Free State Province, South Africa, reveals early gut colonisation and temporal dynamics

Virus Research
The gut of healthy neonates is devoid of viruses at birth, but rapidly becomes colonised by normal viral commensals that aid in important physiological functions like metabolism but can, in some instances, result in gastrointestinal illnesses.
Milton Tshidiso Mogotsi, Ayodeji Emmanuel Ogunbayo, Phillip Armand Bester, Hester Gertruida O'Neill, Martin Munene Nyaga   +4 more
doaj   +1 more source

BATCH-GE : batch analysis of next-generation sequencing data for genome editing assessment [PDF]

, 2016
Targeted mutagenesis by the CRISPR/Cas9 system is currently revolutionizing genetics. The ease of this technique has enabled genome engineering in-vitro and in a range of model organisms and has pushed experimental dimensions to unprecedented proportions.
Boel, Annekatrien, Callewaert, Bert, Coucke, Paul, De Paepe, Anne, De Rocker, Nina, Menten, Björn, Steyaert, Wouter, Willaert, Andy   +7 more
core   +2 more sources

A Cloud Infrastructure for Optimization of a Massive Parallel Sequencing Workflow [PDF]

, 2012
Massive Parallel Sequencing is a term used to describe several revolutionary approaches to DNA sequencing, the so-called Next Generation Sequencing technologies.
Abate, Francesco, Acquaviva, Andrea, Mossucca, L., Provenzano, R., Terzo, Olivier   +4 more
core   +1 more source

Systemic cytokines drive conserved severity-associated myeloid responses across bacterial and viral infections

Communications Biology
Both bacterial and viral infections can trigger an overwhelming host response, leading to immunopathology and organ dysfunction. Multiple studies have reported dysregulated myeloid cell states in patients with bacterial sepsis or severe SARS-CoV-2 ...
Kimberly Kajihara, Donghong Yan, Gretchen L. Seim, Hannah Little-Hooy, Jing Kang, Cynthia Chen, Marco De Simone, Tim Delemarre, Spyros Darmanis, Haridha Shivram, Rebecca N. Bauer, Carrie M. Rosenberger, Sharookh B. Kapadia, Min Xu, Miguel Reyes   +14 more
doaj   +1 more source