Results 31 to 40 of about 978,334 (297)

Metagenomic Analysis of the Enteric RNA Virome of Infants from the Oukasie Clinic, North West Province, South Africa, Reveals Diverse Eukaryotic Viruses

Viruses, 2020
Establishing a diverse gut microbiota after birth is essential for preventing illnesses later in life. However, little knowledge exists about the total viral population (virome) present in the gut of infants during the early developmental stage, with RNA
Milton T. Mogotsi, Peter N. Mwangi, Phillip A. Bester, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Hester G. O’Neill, Martin M. Nyaga   +6 more
doaj   +1 more source

Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells

Frontiers in Immunology, 2022
The transcriptomic profile of a cell population can now be studied at the cellular level using single-cell mRNA sequencing (scRNA-seq). This novel technique provides the unprecedented opportunity to explore the cellular composition of the bronchoalveolar
Sophie E. Sage, Pamela Nicholson, Laureen M. Peters, Tosso Leeb, Tosso Leeb, Vidhya Jagannathan, Vinzenz Gerber   +6 more
doaj   +1 more source

BamView: visualizing and interpretation of next-generation sequencing read alignments. [PDF]

, 2012
So-called next-generation sequencing (NGS) has provided the ability to sequence on a massive scale at low cost, enabling biologists to perform powerful experiments and gain insight into biological processes.
Berriman, Matthew, Carver, Tim, Harris, Simon R., McQuillan, Jacqueline A., Otto, Thomas D., Parkhill, Julian   +5 more
core   +1 more source

Bioinformatics and Next- generation Sequencing [PDF]

Klinicka onkologie, 2015
Next-generation sequencing technologies are currently well-established in the research field and progressively find their way towards clinical applications. Sequencers produce vast amounts of data and therefore bioinformatics methods are needed for processing.
A, Krejčí, P, Müller, B, Vojtěšek
openaire   +2 more sources

Whole Genome Analysis of Human Rotaviruses Reveals Single Gene Reassortant Rotavirus Strains in Zambia

Viruses, 2021
Rotarix® vaccine was implemented nationwide in Zambia in 2013. In this study, four unusual strains collected in the post-vaccine period were subjected to whole genome sequencing and analysis.
Wairimu M. Maringa, Julia Simwaka, Peter N. Mwangi, Evans M. Mpabalwani, Jason M. Mwenda, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Martin M. Nyaga   +7 more
doaj   +1 more source

Next-generation sequencing in dermatology

Frontiers in Medicine, 2023
Over the past decade, Next-Generation Sequencing (NGS) has advanced our understanding, diagnosis, and management of several areas within dermatology. NGS has emerged as a powerful tool for diagnosing genetic diseases of the skin, improving upon traditional PCR-based techniques limited by significant genetic heterogeneity associated with these disorders.
Andrew D. King, Hany Deirawan, Paytra A. Klein, Bahar Dasgeb, Catherine I. Dumur, Darius R. Mehregan   +5 more
openaire   +3 more sources

Whole-Genome Analyses Identifies Multiple Reassortant Rotavirus Strains in Rwanda Post-Vaccine Introduction

Viruses, 2021
Children in low-and middle-income countries, including Rwanda, experience a greater burden of rotavirus disease relative to developed countries. Evolutionary mechanisms leading to multiple reassortant rotavirus strains have been documented over time ...
Sebotsana Rasebotsa, Jeannine Uwimana, Milton T. Mogotsi, Kebareng Rakau, Nonkululeko B. Magagula, Mapaseka L. Seheri, Jason M. Mwenda, M. Jeffrey Mphahlele, Saheed Sabiu, Richard Mihigo, Leon Mutesa, Martin M. Nyaga   +11 more
doaj   +1 more source

Diagnostic applications of next generation sequencing: working towards quality standards [PDF]

, 2012
Over the past 6 years, next generation sequencing (NGS) has been established as a valuable high-throughput method for research in molecular genetics and has successfully been employed in the identification of rare and common genetic variations. All major
Adey, Andrea Gehring, Anna Benet-Pagès, Bainbridge, Bentley, Carsten Bergmann, Clark, Clement, Ding, Dohm, Gerlinger, Gregory, Greif, Greif, Hanno Jörn Bolz, Hanns-Georg Klein, Harismendy, Ina Vogl, Jiang, Johansson, Kaimo Hirv, Kalari, Klaus H. Metzeler, Koboldt, Li, Lister, Loman, Mamanova, Manfred Stuhrmann, Mardis, Marius Kuhn, Mertes, Meyerson, Minoche, Nakamura, Philipp A. Greif, Robinson, Rothberg, Saskia Biskup, Sebastian H. Eck, Shendure, Stefan Kotschote, Stratton   +42 more
core   +1 more source

Seasonal dynamics of DNA and RNA viral bioaerosol communities in a daycare center

Microbiome, 2019
Background Viruses play an important role in ecosystems, including the built environment (BE). While numerous studies have characterized bacterial and fungal microbiomes in the BE, few have focused on the viral microbiome (virome).
Aaron J. Prussin, Pedro J. Torres, John Shimashita, Steven R. Head, Kyle J. Bibby, Scott T. Kelley, Linsey C. Marr   +6 more
doaj   +1 more source

Pharmacogenomics in children: advantages and challenges of next generation sequencing applications [PDF]

, 2013
Pharmacogenetics is considered as a prime example of how personalized medicine nowadays can be put into practice. However, genotyping to guide pharmacological treatment is relatively uncommon in the routine clinical practice. Several reasons can be found
De Paepe, Anne, Vanakker, Olivier
core   +4 more sources