Results 11 to 20 of about 523,633 (260)

High-throughput Quant-iT PicoGreen assay using an automated liquid handling system

BioTechniques, 2019
Workflows in NGS facilities require high-standard practices and high-throughput pipelines to process the large number of samples received in a timely manner. Downstream protocols such as NGS library preparation require accurate estimation of nucleic acid
Kay Anantanawat, Nicola Pitsch, Caroline Fromont, Caroline Janitz   +3 more
doaj   +1 more source

NGSNGS: next-generation simulator for next-generation sequencing data [PDF]

Bioinformatics, 2023
AbstractSummaryWith the rapid expansion of the capabilities of the DNA sequencers throughout the different sequencing generations, the quantity of generated data has likewise increased. This evolution has also led to new bioinformatical methods, for which in silico data have become crucial when verifying the accuracy of a model or the robustness of a ...
Rasmus Amund Henriksen, Lei Zhao, Thorfinn Sand Korneliussen   +2 more
openaire   +3 more sources

Next-generation sequencing [PDF]

Breast Cancer Research, 2009
Next-generation sequencing (also known as massively parallel sequencing) technologies are revolutionising our ability to characterise cancers at the genomic, transcriptomic and epigenetic levels. Cataloguing all mutations, copy number aberrations and somatic rearrangements in an entire cancer genome at base pair resolution can now be performed in a ...
openaire   +2 more sources

Argonaute3-SF3B3 complex controls pre-mRNA splicing to restrain type 2 immunity

Cell Reports, 2023
Summary: Argonaute (AGO) proteins execute microRNA (miRNA)-mediated gene silencing. However, it is unclear whether all 4 mammalian AGO proteins (AGO1, AGO2, AGO3, and AGO4) are required for miRNA activity. We generate Ago1, Ago3, and Ago4-deficient mice (
Riccardo Guidi, Christopher Wedeles, Daqi Xu, Krzysztof Kolmus, Sarah E. Headland, Grace Teng, Joseph Guillory, Yi Jimmy Zeng, Tommy K. Cheung, Subhra Chaudhuri, Zora Modrusan, Yuxin Liang, Stuart Horswell, Benjamin Haley, Sascha Rutz, Christopher Rose, Yvonne Franke, Donald S. Kirkpatrick, Jason A. Hackney, Mark S. Wilson   +19 more
doaj   +1 more source

Uncovering the First Atypical DS-1-like G1P[8] Rotavirus Strains That Circulated during Pre-Rotavirus Vaccine Introduction Era in South Africa

Pathogens, 2020
Emergence of DS-1-like G1P[8] group A rotavirus (RVA) strains during post-rotavirus vaccination period has recently been reported in several countries.
Peter N. Mwangi, Milton T. Mogotsi, Sebotsana P. Rasebotsa, Mapaseka L. Seheri, M. Jeffrey Mphahlele, Valantine N. Ndze, Francis E. Dennis, Khuzwayo C. Jere, Martin M. Nyaga   +8 more
doaj   +1 more source

Genomic Analysis of G2P[4] Group A Rotaviruses in Zambia Reveals Positive Selection in Amino Acid Site 7 of Viral Protein 3

Viruses, 2023
The G2P[4] genotype is among the rotavirus strains that circulate commonly in humans. Several countries have reported its immediate upsurge after the introduction of rotavirus vaccination, raising concern about sub-optimal vaccine effectiveness against ...
Peter N. Mwangi, Robyn-Lee Potgieter, Julia Simwaka, Evans M. Mpabalwani, Jason M. Mwenda, Milton T. Mogotsi, Nonkululeko Magagula, Mathew D. Esona, A. Duncan Steele, Mapaseka L. Seheri, Martin M. Nyaga   +10 more
doaj   +1 more source

A genome-wide survey of mutations in the Jurkat cell line

BMC Genomics, 2018
Background The Jurkat cell line has an extensive history as a model of T cell signaling. But at the turn of the 21st century, some expression irregularities were observed, raising doubts about how closely the cell line paralleled normal human T cells ...
Louis Gioia, Azeem Siddique, Steven R. Head, Daniel R. Salomon, Andrew I. Su   +4 more
doaj   +1 more source

Metagenomic Analysis of the Enteric RNA Virome of Infants from the Oukasie Clinic, North West Province, South Africa, Reveals Diverse Eukaryotic Viruses

Viruses, 2020
Establishing a diverse gut microbiota after birth is essential for preventing illnesses later in life. However, little knowledge exists about the total viral population (virome) present in the gut of infants during the early developmental stage, with RNA
Milton T. Mogotsi, Peter N. Mwangi, Phillip A. Bester, M. Jeffrey Mphahlele, Mapaseka L. Seheri, Hester G. O’Neill, Martin M. Nyaga   +6 more
doaj   +1 more source

Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells

Frontiers in Immunology, 2022
The transcriptomic profile of a cell population can now be studied at the cellular level using single-cell mRNA sequencing (scRNA-seq). This novel technique provides the unprecedented opportunity to explore the cellular composition of the bronchoalveolar
Sophie E. Sage, Pamela Nicholson, Laureen M. Peters, Tosso Leeb, Tosso Leeb, Vidhya Jagannathan, Vinzenz Gerber   +6 more
doaj   +1 more source

Bioinformatics and Next- generation Sequencing [PDF]

Klinicka onkologie, 2015
Next-generation sequencing technologies are currently well-established in the research field and progressively find their way towards clinical applications. Sequencers produce vast amounts of data and therefore bioinformatics methods are needed for processing.
A, Krejčí, P, Müller, B, Vojtěšek
openaire   +2 more sources