Monoclonal Antibody Purification (Nicotiana benthamiana Plants) [PDF]
Bio-Protocol, 2014 Plant-based expression systems provide an alternative biomanufacturing platform for recombinant proteins (Matoba et al., 2011). In particular, plant virus-based vectors can overexpress proteins within days in the leaf tissue of Nicotiana benthamiana (N ...
Adam Husk +2 more
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An innovative infection method for the accumulation of viral nanoparticles in Nicotiana benthamiana [PDF]
Frontiers in Plant ScienceTomato Bushy Stunt Virus (TBSV) naturally infects tomato plants, although it can also infect other plant species, such as Nicotiana benthamiana, a known model system in plant molecular farming.
Kristina Ljumović +5 more
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Molecular Plant-Microbe Interactions, 2011 Citrus tristeza virus (CTV) naturally infects only some citrus species and relatives and within these it only invades phloem tissues. Failure to agroinfect citrus plants and the lack of an experimental herbaceous host hindered development of a workable ...
Silvia Ambrós +6 more
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Transient expression in Nicotiana benthamiana for rapid functional analysis of genes involved in non-photochemical quenching and carotenoid biosynthesis. [PDF]
, 2016 Plants must switch rapidly between light harvesting and photoprotection in response to environmental fluctuations in light intensity. This switch can lead to losses in absorbed energy usage, as photoprotective energy dissipation mechanisms can take ...
Erickson, Erika +3 more
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Transient recombinant expression of highly immunogenic CagA, VacA and NapA in Nicotiana benthamiana
Biotechnology Reports, 2022 Interest in the plant-based transient production of recombinant immunogenic antigens has tremendously progressed because plants are cost-effective, easily selectable, free of mammalian contamination, and support complex post-translational modifications ...
Rambod Barzigar +4 more
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Confocal Microscopy Analysis of Protein Sorting to Plasmodesmata in Nicotiana benthamiana. [PDF]
J Vis ExpKan Y, Simons E, Citovsky V.
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Communications Biology, 2022 The biosynthesis of strictosidine, a key intermediate of monoterpene indole alkaloids, was successfully reconstructed in Nicotiana benthamiana, demonstrating the potential of Nicotiana benthamiana as a bioproduction chassis for small molecules.
Quentin M. Dudley +9 more
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Differential responses to virus challenge of laboratory and wild accessions of australian species of nicotiana, and comparative analysis of RDR1 gene sequences. [PDF]
PLoS ONE, 2015 Nicotiana benthamiana is a model plant utilised internationally in plant virology because of its apparent hyper-susceptibility to virus infection. Previously, others showed that all laboratory accessions of N. benthamiana have a very narrow genetic basis,
Stephen J Wylie +7 more
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Glycoform Modification of Secreted Recombinant Glycoproteins through Kifunensine Addition during Transient Vacuum Agroinfiltration. [PDF]
, 2018 Kifunensine, a potent and selective inhibitor of class I α-mannosidases, prevents α-mannosidases I from trimming mannose residues on glycoproteins, thus resulting in oligomannose-type glycans.
Kailemia, Muchena J +5 more
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C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana. [PDF]
PLoS ONE, 2014 C3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles in various physiological processes of plant life. In this study, a C3HC4-type zinc finger gene was isolated from Nicotiana benthamiana.
Wenxian Wu +4 more
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