THE EFFECT OF MONOCOT INTRONS ON TRANSGENE EXPRESSION IN Nicotiana GENUS PLANTS [PDF]
The aim of the work was to study the effect of introns of the rice OsAct1 and the maize hsp70 genes on the transgene expression in Nicotiana plants in order to find out of their use in the testing of vectors containing these monocot introns. Next methods
I. O. Nitovska +2 more
doaj +1 more source
Evaluation of Crossability between Nicotiana benthamiana and Nicotiana excelsior
Wild tobacco species in the Nicotiana section Suaveolentes are promising genetic resources to introduce their disease resistance to cultivated tobacco, Nicotiana tabacum. However, hybrid lethality is observed in hybrid seedlings from crosses between most
Takahiro Tezuka +3 more
doaj +1 more source
3D‐printed autoclavable plant holders to facilitate large‐scale protein production in plants
The Australian tobacco plant Nicotiana benthamiana is becoming increasingly popular as a platform for protein production and metabolic engineering. In this system, gene expression is achieved transiently by infiltrating N.
Ling Chuang +4 more
doaj +1 more source
Viroids are small, non-coding, parasitic RNAs that promote developmental distortions in sensitive plants. We analyzed pollen of Nicotiana benthamiana after infection and/or ectopic transformation with cDNAs of citrus bark cracking viroid (CBCVd), apple ...
Lenka Steinbachová +5 more
doaj +1 more source
The Receptor-Like Kinase SERK3/BAK1 Is Required for Basal Resistance against the Late Blight Pathogen Phytophthora infestans in Nicotiana benthamiana [PDF]
BACKGROUND The filamentous oomycete plant pathogen Phytophthora infestans causes late blight, an economically important disease, on members of the nightshade family (Solanaceae), such as the crop plants potato and tomato.
Chaparro-Garcia, Angela +8 more
core +1 more source
Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene [PDF]
3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends ...
Zhao Meiwei +4 more
doaj +1 more source
Virus-induced gene silencing database for phenomics and functional genomics in Nicotiana benthamiana [PDF]
Virus-induced gene silencing (VIGS) is an important forward and reverse genetics method for the study of gene function in many plant species, especially Nicotiana benthamiana.
Chakravarthy, Suma +15 more
core +2 more sources
Development of a minigenome cassette for Lettuce necrotic yellows virus: A first step in rescuing a plant cytorhabdovirus [PDF]
Rhabdoviruses are enveloped negative-sense RNA viruses that have numerous biotechnological applications. However, recovering plant rhabdoviruses from cDNA remains difficult due to technical difficulties such as the need for concurrent in planta ...
Drake, P +3 more
core +1 more source
Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]
Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
core +3 more sources
SGR-based Reporter to Assay Plant Transcription Factor-promoter Interactions
We developed an in vivo method to assay plant transcription factor (TF)–promoter interactions using the transient expression system in Nicotiana benthamiana (N. benthamiana) plants.
Shisong Ma, Savithramma Dinesh-Kumar
doaj +1 more source

