Results 81 to 90 of about 24,889 (252)

Real-time PCR Analysis of PAMP-induced Marker Gene Expression in Nicotiana benthamiana

Bio-Protocol, 2018
Perception of pathogen-associated molecular patterns (PAMPs) often triggers various innate immune responses in plants. The transcriptional changes of defense-related genes are often used as a marker to assay PAMP-triggered plant immune response.
Fan Liu, Yuanpeng Xu, Yuanchao Wang, Yuanchao Wang   +3 more
doaj   +1 more source

SIB1‐SEC23A undergo ER to chloroplast relocalization to mediate immunity in Arabidopsis thaliana

Journal of Integrative Plant Biology, EarlyView.
SEC23A mediates membrane trafficking between the endoplasmic reticulum (ER) and Golgi apparatus and interacts with the immunity protein SIGMA FACTOR‐BINDING PROTEIN1 in Arabidopsis. Under stress, both proteins relocate from the ER to chloroplasts, suppressing photosynthesis genes and enhancing defense genes.
Jialin Peng, Huan Zhong, Jianan Zhang, Hailei Zhang, Wuzhen Liu, Yonglun Zeng, Liwen Jiang, Yiji Xia   +7 more
wiley   +1 more source

Functional evaluation of pathogenesis-related protein-1 (PR1) genes in mung bean (Vigna radiata) response to Pythium myriotylum

BMC Genomics
Background Pythium myriotylum Drechsler is considered to be one of the pathogens of mung bean (Vigna radiata (L.) R. Wilczek) root rot, which threatens the yield of V. radiata.
Yanyan Zhou, Yuting Chen, Xinyue Liu, Dong Xue, Yu Zheng, Xingxing Yuan, Qiang Yan, Xin Chen   +7 more
doaj   +1 more source

Chloroplastic metabolic engineering coupled with isoprenoid pool enhancement for committed taxanes biosynthesis in Nicotiana benthamiana

Nature Communications, 2019
Engineering Taxol pathway in microbes needs to overcome the difficulty of expressing plant P450 enzymes. Here, the authors use a compartmentalized metabolic engineering strategy to construct the taxanes production pathway in chloroplasts of Nicotiana ...
Jianhua Li, Ishmael Mutanda, Kaibo Wang, Lei Yang, Jiawei Wang, Yong Wang   +5 more
doaj   +1 more source

The IKU2/RLK7–MAPK–MINI3 signaling module controls endosperm cellularization timing to determine seed size in Arabidopsis

Journal of Integrative Plant Biology, EarlyView.
The phosphorylation status of the WRKY transcription factor WRKY10/MINI3, controls the timing of endosperm cellularization in Arabiopsis, thus regulating final seed size, offering a molecular understanding that could inform future agricultural breeding strategies. ABSTRACT Seed size is a critical factor in determining crop yield.
Min Ni, Yuchen Qian, Tinggang Nong, Wangshu Mou, Niu Liu, La Yang, Dongdong Li, Huixia Shou, Juan Xu   +8 more
wiley   +1 more source

SlMED25‐SlPHR3‐SlSPX2 module fine‐tunes SlPHR3‐mediated transcriptional activation of phosphate starvation response in tomato

Journal of Integrative Plant Biology, EarlyView.
The Mediator subunit SlMED25 and the co‐repressor SlSPX2 competitively bind the N‐terminal domain of the phosphate starvation response transcription factor SlPHR3 in tomato to form a sensitive molecular switch, which dynamically modulates phosphate starvation responses and maintains phosphate homeostasis, offering valuable targets for breeding low ...
Mingtong Zhai, Hongyu Han, Yu Zhang, Yunfeng Zhao, Hongying Guo, Hui Wang, Chuanlong Sun, Xianwen Meng, Lei Deng, Qian Chen, Chuanyou Li   +10 more
wiley   +1 more source

The ZmRACK1–ZmCDPK7–ZmAPX1 module regulates plant antiviral immunity

Journal of Integrative Plant Biology, EarlyView.
In maize, ZmRACK1 acts as a scaffold protein that bridges the cyclin‐dependent kinase ZmCDPK7 and the ascorbate peroxidase ZmAPX1, promoting ZmAPX1 activity to mitigate MCMV infection. Upon viral infection, P31 obstructs formation of the ZmRACK1‐ZmCDPK7‐ZmAPX1 complex, inhibiting ZmCDPK7‐mediated ZmAPX1 activity and promoting reactive oxygen species ...
Yuyang Zhang, Niannian Li, Qiongqiong Wang, Yingchao Sun, Yajuan Shi, Lianwei Yu, Xiaoyu Han, Xiaoxi Feng, Zaifeng Fan, Linlin Chen, Honglian Li, Xue Yang, Hongxia Yuan, Yan Shi   +13 more
wiley   +1 more source

Retardation of the Calvin Cycle Contributes to the Reduced CO2 Assimilation Ability of Rice Stripe Virus-Infected N. benthamiana and Suppresses Viral Infection

Frontiers in Microbiology, 2019
Rice stripe virus (RSV) is naturally transmitted by the small brown planthopper and infects plants of the family Poaceae. Under laboratory conditions, RSV can infect Nicotiana benthamiana by mechanical inoculation, providing a useful system to study RSV ...
Ji’an Bi, Ji’an Bi, Ji’an Bi, Yong Yang, Binghua Chen, Jinping Zhao, Zhuo Chen, Baoan Song, Jianping Chen, Jianping Chen, Fei Yan, Fei Yan   +11 more
doaj   +1 more source

Uncovering the role of the PPR protein PHOTOSYSTEM ONE BIOGENESIS FACTOR6 in splicing chloroplast group II introns

Journal of Integrative Plant Biology, EarlyView.
The P‐class pentatricopeptide repeat (PPR) protein PHOTOSYSTEM ONE BIOGENESIS FACTOR (PBF6) forms splicing complexes with other known splicing factors to facilitate chloroplast intron splicing. PBF6 cooperates with other PPR splicing factors to promote the splicing of the same intron through forming respective splicing complexes.
Mengyu Li, Mengwei Sun, Jinlian Lv, Mingming Song, Siyu Lu, Aihong Zhang, Congming Lu   +6 more
wiley   +1 more source

Detection of Poly (A) RNA in Mesophyll Cells of Nicotiana benthamiana Using in situ Hybridization

Bio-Protocol, 2015
Export of transcribed mRNAs from nucleoplasm to cytosol is an essential process for the translation of genes into proteins. This process is tightly regulated by nuclear pores, composed of about 30 nucleoporin proteins (Nups). Whether or not the mRNAs are
Yuri Mizuno, Daigo Takemoto
doaj   +1 more source