Climate Change Impacts the Structure and Nitrogen-Fixing Activities of Subarctic Feather Moss Microbiomes Across a Precipitation Gradient. [PDF]
Associations between mosses and cyanobacteria are crucial sources of new nitrogen in arctic and subarctic ecosystems. The physiology of both mosses and cyanobacteria is strongly influenced by environmental factors such as temperature and moisture, which directly affect N2 fixation rates.
Alvarenga DO +4 more
europepmc +3 more sources
Constraining uncertainties of diazotroph biogeography from nifH gene abundance [PDF]
AbstractMarine diazotrophs fix dinitrogen gas into bioavailable nitrogen that drives the ocean nitrogen cycle; yet, efforts to infer global diazotroph distributions have been limited by a sparsity of observations. In situ measurements of nifH gene abundance (essential for nitrogen fixation) are increasingly being used to inform the biogeography of ...
Simona Meiler +6 more
openaire +2 more sources
nifH genes in the obligate methane oxidizing bacteria [PDF]
The hifH gene from Klebsiella pneumoniae, which codes for the Fe protein component of nitrogenase, was used as a probe to detect nifH homologues in total cellular DNA from 13 obligate methane oxidizing bacteria. All but one of those strains that had previously been shown capable of fixing dinitrogen contained sequences homologous to nifH.
Christopher J. Oakley, J. Colin Murrell
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Characterization and analysis of nifH genes from Paenibacillus sabinae T27 [PDF]
Paenibacillus sabinae T27 (CCBAU 10202=DSM 17841) is a gram-positive, spore-forming diazotroph with high nitrogenase activities. Three nifH clusters were cloned from P. sabinae T27. Phylogenetic analysis revealed that NifH1, NifH2 and NifH3 cluster with Cyanobacterium. Each of the coding regions of nifH1, nifH2 and nifH3 from P.
Hong, Yuanyuan +5 more
+8 more sources
Rhizobium japonicum nitrogenase Fe protein gene (nifH) [PDF]
A 12.1-kilobase PstI fragment from Rhizobium japonicum, which contains homology to both the Klebsiella pneumoniae and the Rhizobium meliloti nifH genes, was cloned into vector pHE3 . The nifH -homologous region was localized on the restriction enzyme cleavage map by Southern blot hybridization experiments. DNA fragments overlapping the R.
M, Fuhrmann, H, Hennecke
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Detection and characterization of cyanobacterial nifH genes [PDF]
The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594.
J, Ben-Porath, J P, Zehr
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<i>NifH</i> gene amplicon sequencing and metagenomic approaches are complementary in assessing diazotroph diversity. [PDF]
Abstract Exploring the diversity of diazotrophs is key to understanding their role in supplying fixed nitrogen that supports marine productivity. A nested PCR assay using the universal primer set nifH1-nifH4, which targets the nitrogenase (nifH) gene, is a widely used approach for studying marine diazotrophs by amplicon sequencing ...
Cheung S +5 more
europepmc +4 more sources
Exploration of nifH gene through soil metagenomes of the western Indian Himalayas. [PDF]
This group has previously highlighted the prevalence of Csp genes from cold Himalayan environments. However, this study has explored the uncultured diazotrophs from metagenomes of western Indian Himalayas. The metagenomic nifH gene clone library was constructed from the Temperate, Subtropical and Tarai soils of Western Himalaya, India followed by ...
Soni R, Suyal DC, Sai S, Goel R.
europepmc +4 more sources
Comparative phylogeny of rrs and nifH genes in the Bacillaceae [PDF]
The rrs (16S rDNA) gene sequences of nitrogen-fixing endospore-forming bacilli isolated from the rhizosphere of wheat and maize were determined in order to infer their phylogenetic position in the Bacillaceae. These rhizosphere strains form a monophyletic cluster with Paenibacillus azotofixans, Paenibacillus polymyxa and Paenibacillus macerans.
Achouak, Wafa +2 more
openaire +3 more sources
Enhancement of Nitrogenase Activity in Recombinant Escherichia coli by Replacing Upstream Region of nif Operon from Paenibacillus Species. [PDF]
Biological N2 fixation by nitrogenase offers a sustainable alternative to the Haber–Bosch process. Heterologous nitrogenase production in Escherichia coli is a promising strategy. We engineered E. coli with nitrogen fixation (nif) operons from diazotrophic Paenibacillus species.
Honda Y +4 more
europepmc +2 more sources

