Results 211 to 220 of about 6,837 (257)

The Prokaryotic Nitrate Reductases

2007
This chapter reviews the structural organization and bioenergetics of the four prokaryotic NO3 reductases and the eukaryotic enzyme and explores the possible mechanisms of NO3 transport. The membrane-bound NO3- reductase with the active site facing the cytoplasm is usually a three-subunit enzyme composed of NarGHI.
Richardson, David J., Van Spanning, Rob J.M., Ferguson, Stuart J.   +2 more
openaire   +3 more sources

Nitrate reductase regulation: Effects of nitrate and light on nitrate reductase mRNA accumulation

Molecular and General Genetics MGG, 1989
Regulation of nitrate reductase mRNA by light and nitrate was studied in barley seedlings using a partial cDNA clone as a probe. Nitrate reductase mRNA was detected in roots and leaves within 40 min after supplying nitrate to the roots and reached peak accumulation at 2 h in the roots and at 12 h in the leaves.
James M. Melzer, Andris Kleinhofs, Robert L. Warner   +2 more
openaire   +1 more source

The nitrate reductase of Chlorella pyrenoidosa

Biochimica et Biophysica Acta (BBA) - Enzymology, 1971
Abstract 1. 1. A nitrate reductase (NADH:nitrate oxidoreductase, EC 1.6.6.1), which catalyzes the reduction of nitrate to nitrite by NADH, has been found to be present in fresh extracts of nitrate-grown Chlorella pyrenoidosa in the form of a precursor of low activity.
B, Vennesland, C, Jetschmann
openaire   +2 more sources

Properties of a nitrate reductase of Chlorella

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
Abstract 1. An NADH-nitrate oxidoreductase (EC 1.6.6.1) of Chlorella has the unusual property of existing in cell-free extracts mainly in the form of an inactive precursor which can be activated by a variety of procedures. This enzyme is associated with a cytochrome of the b type. 2. The inhibitors, azide, cyanate, thiocyanate and nitrite, react
L P, Solomonson, B, Vennesland
openaire   +2 more sources

Nitrate reductases inEscherichia coli

Antonie van Leeuwenhoek, 1994
Escherichia coli expresses two different membrane-bound respiratory nitrate reductases, nitrate reductase A (NRA) and nitrate reductase Z (NRZ). In this review, we compare the genetic control, biochemical properties and regulation of these two closely related enzyme systems.
V, Bonnefoy, J A, Demoss
openaire   +2 more sources

Histochemical localization of nitrate reductase

Histochemistry, 1981
NADH-dependent nitrate reductase (E.C. 1.6.6.1) was ultrastructurally localized in norflurazon-treated and control soybean cotyledons [Glycine max (L.) Merr.] by a method based upon the increase in osmiophilia due to the formation of an azo dye. The reaction product was observed in small vesicles throughout the cytoplasm.
K C, Vaughn, S O, Duke
openaire   +2 more sources

A method for the determination of nitrate reductase

Journal of Biochemical and Biophysical Methods, 1981
A procedure for the assay of nitrate reductase based on Szekely's diaminodiphenylsulphone method of nitrate determination (Szekely, E. (1967) Talanta 14, 941-950) is described. The method is simple and sensitive, allowing determination of 1 microgram of nitrate in a volume of 1 ml or less.
R M, Daniel, M P, Curran
openaire   +2 more sources

Reductive activation of nitrate reductases

Dalton Transactions, 2005
Protein film voltammetry of Paracoccus pantotrophus respiratory nitrate reductase (NarGH) and Synechococcus elongatus assimilatory nitrate reductase (NarB) shows that reductive activation of these enzymes may be required before steady state catalysis is observed.
Field, Sarah J., Thornton, Nicholas P., Anderson, Lee J., Gates, Andrew J., Reilly, Ann, Jepson, Brian J. N., Richardson, David J., George, Simon J., Cheesman, Myles R., Butt, Julea N.   +9 more
openaire   +3 more sources

[Induced activity of nitrate reductase by nitrate and cloning of nitrate reductase gene].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2010
Excessive nitrate accumulated in plants affects vegetable quality severely and excessive nitrate ingestion would do harm to human health. Assimilatory NADH: nitrate reductase (NR, EC 1.6.6.1), a complex Mo-pterin-, cytochrome b(557)- and FAD-containing protein, catalyzes the regulated and rate-limiting step in the utilization of inorganic nitrogen by ...
Li-Qun, Wang, Yong, Wang, Ying, Dong, Wen-Bing, Wang   +3 more
openaire   +1 more source

Activation of nitrate reductase by oxidation

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1972
Abstract The activation of the inactive form of the nitrate reductase (NADH: nitrate oxidoreductase, EC 1.6.6.1) present in cell-free extracts of Chlorella vulgaris Beijerinck requires an oxidizing agent. Ferricyanide causes conversion of the proenzyme to active enzyme within a few minutes, even at 0°C. Molecular oxygen causes a slow activation which
K, Jetschmann, L P, Solomonson, B, Vennesland   +2 more
openaire   +2 more sources