Results 81 to 90 of about 715,891 (290)

In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols

FEBS Open Bio, EarlyView.
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton, Fang Zhao, Reeta Davis, Meg Walsh, Kevin E O'Connor, David O'Connell, Tanja Narancic   +6 more
wiley   +1 more source

Nitrification a Boon or Curse to the Ecosystem: Nitrification Inhibitors and their Potential for Greener Agriculture

Indian Journal of Microbiology
Nitrification oxidizes ammonia in soil into nitrate, leading to significant nitrogen losses, and reducing nitrogen use efficiency (NUE) in agricultural systems. Majority of applied nitrogen fertilizers contribute to environmental issues resulting from nitrification, denitrification, volatilization, leaching, etc., while only 30-50% are absorbed by ...
Sriram Lakshmanan, Sivasankari Devi Thambusamy, Maheshwari Muthunalliyappan, Rajkishore Subramani Krishnaraj, Shobana Narayanasamy, Vetrimurugan Elumalai, Sivakumar Uthandi   +6 more
openaire   +2 more sources

Effect of the application of cattle urine with or without the nitrification inhibitor DCD, and dung on greenhouse gas emissions from a UK grassland soil [PDF]

, 2016
Emissions of nitrous oxide (N2O) from soils from grazed grasslands have large uncertainty due to the great spatial variability of excreta deposition, resulting in heterogeneous distribution of nutrients.
Donovan, N., Cardenas, L. M., Misselbrook, T. H., Chadwick, D. R., Hodgson, C. J., Smith, K. A., Dhanoa, M. S., Gilhespy, S. L., Gilhespy, Sarah, Hodgson, Chris, Misselbrook, Tom, Chadwick, Dave, Cardenas, Laura, Donovan, Neil   +13 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

FEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima, Kotone Watanabe, Grigorii Sultanakhmetov, Aoi Fukuchi, Keiya Ito, Sawako Shimizu, Taro Saito, Akiko Asada, Kanae Ando   +8 more
wiley   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora

FEBS Open Bio, EarlyView.
This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi, Alain Dolla, Frauke Baymann, Celine Rommevaux, Latifa Elantak, Helene Gaussier, Matthieu Nouailler   +6 more
wiley   +1 more source

Some plant extracts retarde nitrification in soil

Acta Agriculturae Slovenica, 2014
An incubation experiment was conducted to evaluate the effect of aqueous extracts of 17 plant materials on nitrification inhibition of urea- N in soil as compared with chemical inhibitor Dicyandiamide (DCD).
Abdul–Mehdi S. AL-ANSARI, Mohammed A. ABDULKAREEM   +1 more
doaj   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Susceptibility of Ammonia-Oxidizing Bacteria to Nitrification Inhibitors

Zeitschrift für Naturforschung C, 2003
Activity of nitrification inhibitors to several typical ammonia-oxidizing bacteria isolated recently, i. e. Nitrosococcus, Nitrosolobus, Nitrosomonas, Nitrosospira and Nitrosovibrio species was assayed using 2-amino-4-methyl-6-trichloromethyl-1,3,5-triazine (MAST), 2-amino- 4-tribromomethyl-6-trichloromethyl-1,3,5-triazine (Br-MAST), 2-chloro-6 ...
Douchi, Matsuba, Hirotoshi, Takazaki, Yukiharu, Sato, Reiji, Takahashi, Tatsuaki, Tokuyama, Ko, Wakabayashi   +5 more
openaire   +2 more sources