Results 31 to 40 of about 11,182 (261)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

open access: yesFEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho   +3 more
wiley   +1 more source

Initial evidence on the effect of copper on global cropland nitrogen cycling: A meta-analysis

open access: yesEnvironment International
Copper (Cu) is a key cofactor in ammonia monooxygenase functioning responsible for the first step of nitrification, but its excess availability impairs soil microbial functions and plant growth.
Ahmed S. Elrys   +12 more
doaj   +1 more source

Ammonia Removal by Simultaneous Nitrification and Denitrification in a Single Dual-Chamber Microbial Electrolysis Cell

open access: yesEnergies, 2022
Ammonia removal from wastewater was successfully achieved by simultaneous nitrification and denitrification (SND) in a double-chamber microbial electrolysis cell (MEC).
Sanath Kondaveeti   +3 more
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

open access: yesFEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy   +4 more
wiley   +1 more source

Relative Abundance of Ammonia Oxidizing Archaea and Bacteria Influences Soil Nitrification Responses to Temperature

open access: yesMicroorganisms, 2019
Ammonia oxidizing archaea (AOA) and bacteria (AOB) are thought to contribute differently to soil nitrification, yet the extent to which their relative abundances influence the temperature response of nitrification is poorly understood.
Hussnain Mukhtar   +3 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Salmonella lipopolysaccharide‐containing supported lipid bilayers as platforms to study bacteriophage interactions

open access: yesFEBS Letters, EarlyView.
We present robust protocols for the preparation of supported lipid bilayers (SLBs) incorporating either Salmonella smooth LPS or outer membrane vesicles (OMVs). We use a combination of quartz crystal microbalance with dissipation (QCM‐D) and fluorescence microscopy to both characterize the SLBs of various compositions and to probe their interactions ...
Hudson P. Pace   +6 more
wiley   +1 more source

Determination of rate parameter for kinetics of nitrification

open access: yesJurnal Teknik Kimia Indonesia, 2018
Determination of rate parameter for kinetics of nitrification The nitrification process is the bottleneck step in the total nitrogen removal. The formation of nitrate is considered as the rate limiting step in the whole process and its kinetics determine the design of the nitrification reactor.
S R Juliastuti   +3 more
openaire   +2 more sources

Three phosphatase families form a community: The phosphohydrolases that act upon inositol pyrophosphates

open access: yesFEBS Letters, EarlyView.
Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley   +1 more source

Development of N2O emission factors derived from nitrification of treated sewage water in rivers

open access: yesWater Science and Technology, 2023
Because treated sewage water contains nitrogen compounds such as NH4-N and NO2+3-N, nitrous oxide (N2O) which is one of the greenhouse gases is generated by nitrification and denitrification reactions in rivers.
Hiroshi Yamazaki   +4 more
doaj   +1 more source

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