Results 51 to 60 of about 1,099,567 (306)

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

open access: yesFEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee   +3 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

open access: yesFEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa   +5 more
wiley   +1 more source

Determination of Nitrate Uptake and Accumulation Using 15N in Rice Seedlings

open access: yesBio-Protocol, 2013
Nitrogen-15 is a rare stable isotope of nitrogen. This isotope is often used in agricultural research. For example, Nitrogen-15 is used to trace mineral nitrogen compounds and translocate the nitrogen molecule in plants.
Zhong Tang, Guohua Xu
doaj   +1 more source

The Nitrogen Budget of Coastal Eastern Guangdong in the Last 15 Years

open access: yesHydrology, 2021
Nitrogen pollution has caused severe ecological and environmental crisis, especially in densely populated coastal regions. Using a mathematical model based on statistical data series from industry, agriculture, environmental protection, and population in
Yongsong Su   +3 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

open access: yesFEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel   +13 more
wiley   +1 more source

Assessing the Systematic Effects of the Concentration of Nitrogen Supplied to Dual-Root Systems of Soybean Plants on Nodulation and Nitrogen Fixation

open access: yesAgronomy, 2020
The specific mechanisms by which nitrogen affects the nodulation and nitrogen fixation of soybean plants are unclear. Investigating the relationship between nitrogen, nodulation and nitrogen fixation can provide new insights for the rational and proper ...
Xiaochen Lyu   +6 more
doaj   +1 more source

Chronologically sampled flight feathers permits recognition of individual molt-migrants due to varying protein sources [PDF]

open access: yesPeerJ, 2015
This is a proof of concept paper based on chronological samples of growing feathers from geese thought to be molt-migrants. When molt-migrant birds initiate molt shortly after migrating to a new isoscape, isotope values measured along the length of their
Sievert Rohwer   +3 more
doaj   +2 more sources

Layered-Strip Fertilization Improves Nitrogen Use Efficiency by Enhancing Absorption and Suppressing Loss of Urea Nitrogen

open access: yesAgronomy, 2023
Appropriate deep application of fertilizer is the key basis for improving nitrogen use efficiency (NUE). However, the effects of different deep application methods and fertilizer types on nutrient migration, NUE and biomass in wheat season are unclear ...
Hongliang Wu   +5 more
doaj   +1 more source

Nitrogen isotope analysis of ammonium and glycine [PDF]

open access: yes, 2005
Stable isotope techniques can be used as a tool in nitrogen cycling studies of different ecosystems. The studies are based on measurement of the heavy (15N)- to- light (14N) isotopic ratios of nitrogen in different biospheric pools.
Norlin, Elin
core  

pH‐mediated activation of the lysosomal arginine sensor SLC38A9

open access: yesFEBS Letters, EarlyView.
Cells monitor nutrient levels via the lysosomal transporter SLC38A9 to activate the mechanistic target of rapamycin complex 1 (mTORC1). This study reveals that SLC38A9 function is regulated by pH. We identified histidine 544 as a critical pH sensor that undergoes conformational changes to control amino acid efflux from lysosomes; therefore, it ...
Xuelang Mu, Ampon Sae Her, Tamir Gonen
wiley   +1 more source

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