Numerical simulation of ammonia refueling process for ammonia-powered vehicles
You-qi chuyunObjectiveAmmonia energy, a zero-carbon carrier, is emerging as a key solution for the green transformation of transportation, including vehicles and ships.
Lin TENG +7 more
doaj +1 more source
Long Spin Coherence Times of Nitrogen Vacancy Centres in Milled Nanodiamonds
, 2021 B. D. Wood +10 more
openalex +1 more source
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Frontiers in Bioscience-EliteBackground: Rhizobial inoculation in combination with fungicidal seed treatment is an effective solution for improving soybean resistance to modern climate changes due to the maximum implementation of the plant’s stress-protective antioxidant properties ...
Tetiana Nyzhnyk +2 more
doaj +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Erratum: Nitrogen-ion energy loss in Havar, nickel, Kapton, and Mylar foils [PDF]
, 1988 Anonymous
openalex +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
The Abundance of Low Energy Cosmic Ray Boron and Nitrogen Nuclei Measured at Voyager 1 Beyond the Heliopause, Where Do They All Come From? An Interpretation Using a Leaky Box Galactic Propagation Model [PDF]
, 2015 W. R. Webber
openalex +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
FEBS Open Bio, EarlyView.We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source