Solid-state NMR spectroscopy on complex biomolecules [PDF]
, 2010 Biomolecular applications of NMR spectroscopy are often merely associated with soluble molecules or magnetic resonance imaging. However, since the late 1970s, solid-state NMR (ssNMR) spectroscopy has demonstrated its ability to provide atomic-level ...
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Binding hotspots of BAZ2B bromodomain:histone interaction revealed by solution NMR driven docking [PDF]
, 2014 Bromodomains are epigenetic reader domains, which have come under increasing scrutiny both from academic and pharmaceutical research groups. Effective targeting of the BAZ2B bromodomain by small molecule inhibitors has been recently reported, but no ...
Ferguson, Fleur M. +26 more
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CASD-NMR: critical assessment of automated structure determination by NMR [PDF]
, 2009 NMR spectroscopy is currently the only technique for determining the solution structure of biological macromolecules. This typically requires both the assignment of resonances and a labor-intensive analysis of multidimensional nuclear Overhauser effect ...
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Nuclear magnetic resonance (NMR) applied to membrane-protein complexes [PDF]
, 2016 Increasing evidence suggests that most proteins occur and function in complexes rather than as isolated entities when embedded in cellular membranes.
Baldus, Marc +5 more
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Flow injection analysis NMR (FIA–NMR): a novel flow NMR technique that complements LC–NMR and direct injection NMR (DI–NMR) [PDF]
Magnetic Resonance in Chemistry, 2003 AbstractDetails of a new flow NMR technique, flow injection analysis NMR (FIA–NMR), are presented for the first time. This method blends some aspects of both liquid chromatography–NMR and direct injection NMR, and complements both. FIA‐NMR is shown to be useful as an analytical technique, especially for repetitive analyses, and may also prove useful in
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Formation of the β-barrel assembly machinery complex in lipid bilayers as seen by solid-state NMR
Nature Communications, 2018 The β-barrel assembly machinery (BAM) catalyzes β-barrel protein insertion into the outer membrane of E.coli. Here authors employ high-sensitivity solid-state NMR to reveal how the lipid environment and formation of the BamA-BamCDE complex affect BamA ...
Cecilia Pinto +5 more
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Development of Genistein Synthesis for Use as a Certified Reference Material
Разработка и регистрация лекарственных средств, 2021 Introduction. The use of certified reference materials (CRMs) ensures metrological traceability and comparability of analysis results performed in different laboratories, by different analysts, at different times.
A. A. Zhigalina +3 more
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A NMR guided approach for CsrA–RNA crystallization [PDF]
, 2013 Structure determination of protein–nucleic acid complexes remains a challenging task. Here we present a simple method for generating crystals of a CsrA–nucleic acid complex, guided entirely by results from nuclear magnetic resonances spectroscopy (NMR ...
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Chemical shift extremum of 129Xe(aq) reveals details of hydrophobic solvation
Scientific Reports, 2018 The 129Xe chemical shift in an aqueous solution exhibits a non-monotonic temperature dependence, featuring a maximum at 311 K. This is in contrast to most liquids, where the monotonic decrease of the shift follows that of liquid density.
Petri Peuravaara +5 more
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Information-driven modeling of large macromolecular assemblies using NMR data [PDF]
, 2014 Availability of high-resolution atomic structures is one of the prerequisites for a mechanistic understanding of biomolecular function. This atomic information can, however, be difficult to acquire for interesting systems such as high molecular weight ...
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