Results 51 to 60 of about 922,338 (264)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Study on the influence of working condition variation on lubrication characteristics of EHA internal pump

Scientific Reports
The operational characteristics of Electro-Hydraulic Actuator(EHA) result in the EHA internal pump facing complex conditions of variable speed, pressure and even direction, resulting in poor lubrication state of friction pairs.
Yantao Zhang, Zongbin Chen, Jun Che, Jian Liao   +3 more
doaj   +1 more source

Intelligent Alignment Control for Floating Raft Air Spring Mounting System Under Coupled Hull–Raft Deformation

Journal of Marine Science and Engineering
Shaft alignment is adversely affected by the increasingly severe coupled hull–raft deformation in deep-diving, highly integrated submersibles, thereby compromising operational safety and potentially amplifying vibration noise.
Jian-Wei Cheng, Wen-Jun Bu, Ze-Chao Hu, Jun-Qiang Fu, Hong-Rui Zhang, Liang Shi   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source

ESL-YOLO: Edge-Aware Side-Scan Sonar Object Detection with Adaptive Quality Assessment

Journal of Marine Science and Engineering
Focusing on the problem of insufficient detection accuracy caused by blurred target boundaries, variable scales, and severe noise interference in side-scan sonar images, this paper proposes a high-precision detection network named ESL-YOLO, which ...
Zhanshuo Zhang, Changgeng Shuai, Chengren Yuan, Buyun Li, Jianguo Ma, Xiaodong Shang   +5 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Gas–liquid coupling fluid pulsation attenuator: Theoretical and experimental studies

Applied Ocean Research
In response to low-frequency fluid pulsation suppression demand in ship hydraulic pipeline systems, a gas–liquid coupling type fluid pulsation attenuator (GLC-FPA) is proposed.
Jian Liao, Fuming Zhou, Lin He, Zongbin Chen, Renyuan Wang, Xiaopeng Tan, Yundong Liang, Yuanzhi Xu   +7 more
doaj   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Stiffness analysis and structural optimization design of an air spring for ships

Scientific Reports
An air spring (AS) for ships must have the structural strength of its bellows enhanced considerably to ensure its reliability under high internal pressure and strong impact.
Yuqiang Cheng, Hua Gao, Jianguo Ma, Changgeng Shuai   +3 more
doaj   +1 more source