Results 131 to 140 of about 262,938 (280)
Frontiers in Cell and Developmental BiologyDelivery to the correct membrane domain in polarized epithelial cells is a critical regulatory mechanism for transmembrane proteins. The trafficking of these proteins is directed by short amino acid sequences known as sorting motifs. In six basolaterally-
Sarah J. Harmych +6 more
doaj +1 more source
Considerations for the genotoxicity assessment of middle size peptide drugs containing non-canonical amino acid residues. [PDF]
Genes Environ, 2023 Mishima M, Sugiyama KI.
europepmc +1 more source
Isolation Defines Identity: Functional Consequences of Extracellular Vesicle Purification Strategies
Advanced Healthcare Materials, EarlyView.Four extracellular vesicle purification strategies are compared using ovarian‐cancer ascites and ES‐2 cell supernatants. A novel workflow links purification to function by combining particle‐normalized proteomics with matched cell‐free and cell‐based assays.
Christian Preußer +10 more
wiley +1 more source
Protein engineering and design with non canonical amino acids [PDF]
, 2004 The translation machinery of cells can be exploited for the incorporation of various non canonical amino acids into proteins. In this work, different fluoro-, amino-, and methyl- containing Tryptophan and Leucine analogues were incorporated into model proteins by the use of selective pressure incorporation (SPI) method.
openaire +1 more source
Advanced Healthcare Materials, EarlyView.Osteogenic‐angiogenic cross‐talk is a vital prerequisite for vascularized bone regeneration. In this study, we investigated the effects of siRNA‐mediated silencing of two inhibitory proteins, Chordin and WWP‐1, via CaP‐NP‐loaded gelatin microparticles in osteogenically differentiated microtissues.
Franziska Mitrach +7 more
wiley +1 more source
Design and Synthesis of Peptide‐Polyester Conjugates for Cell‐Mediated Scaffold Degradation
Advanced Healthcare Materials, EarlyView.This work describes polycaprolactone (PCL)‐based biomaterials engineered to degrade in response to cell‐secreted proteases. A fast‐degrading peptide (Fast) sequence is integrated into a PCL conjugate backbone to produce a biomaterial that is selectively degraded by multiple cell types compared to its scrambled control (ScrFast).
Korina Vida G. Sinad +7 more
wiley +1 more source
Advanced Healthcare Materials, EarlyView.The biomimetic nanoplatform IMNP (ETS1 pDNA/PBAE@ITP‐MM) undergoes targeted disassembly at inflammatory vascular sites to release the ETS1 plasmid (pETS1). This release initiates a cascade of effects that inhibit pathogenic pathways and support immune homeostasis. (Abbreviations: EndMT, endothelial‐to‐mesenchymal transition; EC, endothelial cell; TC, T
Feng Zhang +13 more
wiley +1 more source
Multiplexed Assessment of Promiscuous Non-Canonical Amino Acid Synthase Activity in a Pyridoxal Phosphate-Dependent Protein Family. [PDF]
ACS Catal, 2023 Zmich A +4 more
europepmc +1 more source
Advanced Healthcare Materials, EarlyView.This study presents the first entirely isogenic heart‐on‐chip, unifying cardiomyocytes, fibroblasts, and endothelial cells from a single iPSC source. The platform reveals a critical biological insight: the endothelium actively shields cardiac tissue from drug‐induced toxicity, challenging the predictive accuracy of conventional, avascular models for ...
Karine Tadevosyan +12 more
wiley +1 more source
p-Chloropropynyl Phenylalanine, a Versatile Non-Canonical Amino Acid for Co-Translational Peptide Macrocyclization and Side Chain Diversification. [PDF]
Chembiochem, 2023 Osorio Franco HE +3 more
europepmc +1 more source