Results 141 to 150 of about 12,851,211 (266)

Non‐canonical amino acid bioincorporation into antimicrobial peptides and its challenges

Journal of Peptide Science
The rise of antimicrobial resistance and multi‐drug resistant pathogens has necessitated explorations for novel antibiotic agents as the discovery of conventional antibiotics is becoming economically less viable and technically more challenging for biopharma.
George Nkrumah Enninful, Rajesh Kuppusamy, Elvis K. Tiburu, Naresh Kumar, Mark D. P. Willcox   +4 more
openaire   +2 more sources

Genetic Code Expansion for Controlled Surfactin Production in a High Cell-Density Bacillus subtilis Strain

Microorganisms
Background: In biotechnology, B. subtilis is established for heterologous protein production. In addition, the species provides a variety of bioactive metabolites, including the non-ribosomally produced surfactin lipopeptide.
Alexander Hermann, Eric Hiller, Philipp Hubel, Lennart Biermann, Elvio Henrique Benatto Perino, Oscar Paul Kuipers, Rudolf Hausmann, Lars Lilge   +7 more
doaj   +1 more source

Engineering Pluripotent Stem Cells‐Derived Inner Ear Organoids With Enhanced Maturation and Reproducibility by Micro‐Topographical Cues

Advanced Materials, EarlyView.
Micro‐topographical cues applied through temporally controlled microscale confinement improve the reproducibility, spatial organization, and neurosensory‐associated features of pluripotent stem cell‐derived inner ear organoids. Integration with a vascularized organoid platform further enables controlled investigation of vascular‐epithelial interactions
Harshita Sharma, Jungeun Lim, Woochan Kim, Yeon Ju Kim, Dream Kim, Shinyull Lee, Chaeyeon Park, Stephen Rhee, Shruthy Kuttapan, Jungho Ha, Sang Wook Park, Kyunghoon Kim, Noo Li Jeon, Sunho Park, Yun‐Hoon Choung, Jangho Kim   +15 more
wiley   +1 more source

All‐Amyloid Functional Coacervates

Advanced Materials, EarlyView.
This study introduces an all‐amyloid interfacial complex coacervate technique that employs charge‐mediated or functionalization‐assisted complexation of oppositely charged food protein‐derived amyloid nanofibrils to engineer functional soft constructs across multiple length scales.
Seyyed Alireza Hashemi, Mohammad Peydayesh, Laura Baraldi, Mohammad Arjmand, Raffaele Mezzenga   +4 more
wiley   +1 more source

Ribosome selectivity and nascent chain context in modulating the incorporation of fluorescent non-canonical amino acid into proteins. [PDF]

Sci Rep, 2022
Thommen M, Draycheva A, Rodnina MV.
europepmc   +1 more source

Ionic Gating of Liquid‐Crystal‐Like Pteridine Assemblies Enables Tunable Light Scattering

Advanced Materials, EarlyView.
Tunable optical properties emerge through developmentally regulated ionic gating, which transforms disordered, ultraviolet (UV)‐absorbing organelles into concentrically ordered, liquid‐crystal‐like light scatterers. By integrating analyses of pteridine composition, potassium‐dependent assembly, hierarchical ultrastructure, and optical behavior, the ...
Sourabh Bera, Zohar Eyal, Yael Noy, Yuval Barzilay, Siddharth Sahoo, Srikant Moharana, Avi Baram, Tali Lerer‐Goldshtein, Neta Varsano, Bar Bader, Lothar Houben, Uwe Heinig, Iddo Pinkas, Moshe Goldsmith, Tamar Unger, Shira Albeck, Andrea Sorrentino, Maxim Itkin, Sergey Malitsky, Ziv Porat, Ido Hadar, Venkata Jayasurya Yallapragada, Dvir Gur   +22 more
wiley   +1 more source

Rab1A Promotes Hepatic Steatosis by Suppressing Mitophagy via the Raf‐1/ERK1/2/PINK1 Signaling Axis

Advanced Science, EarlyView.
Why does fat accumulate in the liver? Our study reveals Rab1A as the molecular switch that silences the cell's mitochondrial cleanup crew. Turning Rab1A off reactivates mitophagy, clears excess fat, and reverses fatty liver disease in mice—offering a promising new therapeutic target for MASLD, a growing global health challenge.
Li Zhang, Jianhua Li, Huilu Zhang, Qi Qin, Yichen Huang, Xiaofan Tian, Chao Sun, Binbin Li, Zhengxin Wang, Xin Zhang   +9 more
wiley   +1 more source

Beyond the Canonical 20: Peptide Discovery with Non-Canonical Amino Acids

CHIMIA
Amino acids are central to biology as signaling molecules and as the building blocks of peptides and proteins, which represent an expanding class of drugs with vast therapeutic potential. The precise modulation of individual residues in therapeutic peptides and proteins is crucial to enhance their pharmacological properties. Expanding beyond the twenty
openaire   +2 more sources

Sparse pseudocontact shift NMR data obtained from a non-canonical amino acid-linked lanthanide tag improves integral membrane protein structure prediction. [PDF]

J Biomol NMR, 2023
Ledwitch KV, Künze G, McKinney JR, Okwei E, Larochelle K, Pankewitz L, Ganguly S, Darling HL, Coin I, Meiler J.   +9 more
europepmc   +1 more source

Innate Immunocompetent hiPSC‐Derived Neurospheroids Capture Early CNS Responses to rAAV

Advanced Science, EarlyView.
Knowledge of human CNS immune responses to AAV‐based gene therapies remains limited due to the lack of immune‐competent human models. Here, a hiPSC‐derived 3D neuroimmune platform integrating neurospheroids and microglia is established using stirred‐tank bioreactors.
Catarina M. Gomes, Gabriela Silva, Mafalda Aleixo, Marta Gomes, Daniel Simão, Stephan J. Holtkamp, Diana D. Lobo, Pradeep Harish, Rosalind Jenkins, Lekh N. Dahal, Rui J. Nobre, Luís de Pereira de Almeida, Mark Trautwein, Paula M. Alves, Catarina Brito   +14 more
wiley   +1 more source