Expanding the genetic code of Salmonella with non-canonical amino acids [PDF]
Scientific Reports, 2016 AbstractThe diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnological applications. The genetic code expansion strategy, which co-translationally incorporates ncAAs into specific sites of target proteins, has been applied in many organisms. However, there have been only few
Qinglei Gan +3 more
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Molecules, 2014 In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code.
Ying Ma +4 more
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Non-canonical proteolytic activation of human prothrombin by subtilisin from Bacillus subtilis may shift the procoagulant\ue2\u80\u93anticoagulant equilibrium toward thrombosis [PDF]
, 2017 Blood coagulation is a finely regulated physiological process culminating with the factor Xa (FXa)-mediated conversion of the prothrombin (ProT) zymogen to active -thrombin (T).
Acquasaliente, Laura +5 more
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Leucyl-tRNA Synthetase Activates Vps34 in Amino Acid-Sensing mTORC1 Signaling
Cell Reports, 2016 Amino acid availability activates signaling by the mammalian target of rapamycin (mTOR) complex 1, mTORC1, a master regulator of cell growth. The class III PI-3-kinase Vps34 mediates amino acid signaling to mTORC1 by regulating lysosomal translocation ...
Mee-Sup Yoon +5 more
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Incorporation of non-canonical amino acids into the developing murine proteome [PDF]
Scientific Reports, 2016 AbstractAnalysis of the developing proteome has been complicated by a lack of tools that can be easily employed to label and identify newly synthesized proteins within complex biological mixtures. Here, we demonstrate that the methionine analogs azidohomoalanine and homopropargylglycine can be globally incorporated into the proteome of mice through ...
Sarah Calve +3 more
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Functional analysis and binding affinity of tomato ethylene response factors provide insight on the molecular bases of plant differential responses to ethylene [PDF]
, 2012 Background : The phytohormone ethylene is involved in a wide range of developmental processes and in mediating plant responses to biotic and abiotic stresses.
Bouzayen, Mondher +10 more
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Conformational plasticity of NaK2K and TREK2 potassium channel selectivity filters
Nature Communications, 2023 The potassium channel selectivity filter is responsible for conduction and selectivity of K + over other cations. Here, the authors use a combination of single molecule FRET, non-canonical fluorescent amino acid incorporation, and single channel patch ...
Marcos Matamoros +4 more
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Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?
Biomolecules, 2023 Amino acid antiporters mediate the 1:1 exchange of groups of amino acids. Whether substrate specificity can be different for the inward and outward facing conformation has not been investigated systematically, although examples of asymmetric transport ...
Gregory Gauthier-Coles +3 more
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To Be or Not To Be a Molecular Ion: The Role of the Solvent in Photoionization of Arginine. [PDF]
, 2019 Application of photoionization mass spectroscopy, a technique capable of assessing protonation states in complex molecules in the gas phase, is challenging for arginine due to its fragility. We report photoionization efficiencies in the valence region of
Ahmed, Musahid +7 more
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Cells, 2022 Targeting cancer metabolism has become one of the strategies for a rational anti-tumor therapy. However, cellular plasticity, driven by a major regulator of cellular growth and metabolism, mTORC1, often leads toward treatment resistance.
Biljana Blagojevic +3 more
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