Results 41 to 50 of about 12,851,211 (266)

The genetic incorporation of thirteen novel non-canonical amino acids [PDF]

Chem. Commun., 2014
Thirteen novel non-canonical amino acids were synthesized and tested for suppression of an amber codon using a mutant pyrrolysyl-tRNA synthetase–tRNAPylCUA pair.
Alfred, Tuley, Yane-Shih, Wang, Xinqiang, Fang, Yadagiri, Kurra, Yohannes H, Rezenom, Wenshe R, Liu   +5 more
openaire   +2 more sources

Expanding the genetic code of Salmonella with non-canonical amino acids [PDF]

Scientific Reports, 2016
AbstractThe diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnological applications. The genetic code expansion strategy, which co-translationally incorporates ncAAs into specific sites of target proteins, has been applied in many organisms. However, there have been only few
Qinglei Gan, Brent P. Lehman, Thomas A. Bobik, Chenguang Fan   +3 more
openaire   +2 more sources

Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool

Scientific Reports, 2023
Modification of proteins with a broad range of chemical functionalities enables the investigation of protein structure and activity by manipulating polypeptides at single amino acid resolution.
Jeffrey L. Schloßhauer, Anne Zemella, Srujan K. Dondapati, Lena Thoring, Manpreet Meyer, Stefan Kubick   +5 more
doaj   +1 more source

Diel rhythmicity in amino acid uptake by Prochlorococcus [PDF]

, 2008
The marine cyanobacterium Prochlorococcus, the most abundant phototrophic organism on Earth, numerically dominates the phytoplankton in nitrogen (N)-depleted oceanic gyres.
Terry, Matthew J, Garczarek, Laurence, Tarran, Glen A, Mary, Isabelle, Burkill, Peter H, Scanlan, David J, Kolowrat, Christian, Zubkov, MV, Zubkov, Mikhail V, Terry, MJ, Tarran, Glen A., Scanlan, David J., Scanlan, DJ, Burkill, Peter H., Tarran, GA, Terry, Matthew J., Burkill, PH, Zubkov, Mikhail V., Kolowrat, CK, Mary, I, Garczarek, LG   +20 more
core   +1 more source

Incorporation of non-canonical amino acids into the developing murine proteome [PDF]

Scientific Reports, 2016
AbstractAnalysis of the developing proteome has been complicated by a lack of tools that can be easily employed to label and identify newly synthesized proteins within complex biological mixtures. Here, we demonstrate that the methionine analogs azidohomoalanine and homopropargylglycine can be globally incorporated into the proteome of mice through ...
Sarah Calve, Andrew J. Witten, Alexander R. Ocken, Tamara L. Kinzer-Ursem   +3 more
openaire   +2 more sources

Amino acid uptake in Arabidopsis [PDF]

, 2008
Nitrogen (N) is essential for all living organisms and is considered to be the limiting factor for plant growth in many ecosystems. Although generally believed to rely on mineral N to fulfill their N needs, plants have also been found to access organic N
Svennerstam, Henrik
core   +1 more source

Root phloem-specific expression of the plasma membrane amino acid proton co-transporter AAP3 [PDF]

, 2004
Amino acids are regarded as the nitrogen ‘currency’ of plants. Amino acids can be taken up from the soil directly or synthesized from inorganic nitrogen, and then circulated in the plant via phloem and xylem.
Sakiko Okumoto, Leister, D., Frommer, Wolf B., Wolf B. Frommer, Okumoto, Sakiko, FISCHER, Wolf N, Stierhof, York Dieter, Frommer, W. B., Stierhof, Y., Stierhof, Y.-D., Fischer, W., Wolfgang Koch, Koch, W., FROMMER, Wolf B, Mechthild Tegeder, Fischer, Wolf N., Leister, Dario, Wolf N. Fischer, Koch, Wolfgang, Okumoto, S., Tegeder, Mechthild, Dario Leister, Tegeder, M., Biehl, A., York Dieter Stierhof, Alexander Biehl, Biehl, Alexander, Fischer, W. N., Frommer, W.   +28 more
core   +2 more sources

Mechanistic Investigations of Receptor Signaling via Canonical and Non-Canonical Amino Acid Mutagenesis [PDF]

, 2019
This dissertation primarily describes investigations of the mechanisms by which pentameric ligand-gated ion channels (pLGICs) activate ("gating") using canonical and non-canonical amino acid mutagenesis.
Mosesso, Richard Albert
core   +1 more source

AMINO ACID PROFIL OF EARTHWORM AND EARTHWORM M AL (Lumbricus rubellus )FOR ANIMAL F DSTUFF [PDF]

, 2009
Earthworm meal (Lumbricus rubellus has become one of natural material that could be used as feed additive.Powdering method of earthworm was done by using formic acid addition.The study was carried out (1)to evaluate the essential amino acid profile of ...
Sofyan, A, Damayanti, E, Julendra, H, Istiqomah, L   +3 more
core   +1 more source

Cell-Free Approach for Non-canonical Amino Acids Incorporation Into Polypeptides [PDF]

Frontiers in Bioengineering and Biotechnology, 2020
Synthetic biology holds promise to revolutionize the life sciences and biomedicine via expansion of macromolecular diversity outside the natural chemical space. Use of non-canonical amino acids (ncAAs) via codon reassignment has found diverse applications in protein structure and interaction analysis, introduction of post-translational modifications ...
Cui, Zhenling, Johnston, Wayne A., Alexandrov, Kirill   +2 more
openaire   +4 more sources