Structural Basis for Blocked Excited State Proton Transfer in a Fluorescent, Photoacidic Non-Canonical Amino Acid-Containing Antibody Fragment. [PDF]
J Mol Biol, 2022 Henderson JN, Simmons CR, Mills JH.
europepmc +2 more sources
A Dual-Purpose Non-Canonical Amino Acid for the Expanded Genetic Code: Combining Metal-Binding and Click Chemistry. [PDF]
Angew Chem Int Ed EnglDay GJ +4 more
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Amino acid racemization dating: Method development using African ostrich (Struthio camelus) eggshell [PDF]
, 2013 Ostrich eggshell (OES) is ubiquitous in archaeological sites in Africa and is a favoured substrate for amino acid racemisation (AAR) geochronology, yielding consistent results and thought to approximate a closed system with respect to protein diagenesis.
Crisp, Molly Katherine
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An in vivo photo-cross-linking approach reveals a homodimerization domain of Aha1 in S. cerevisiae. [PDF]
PLoS ONE, 2014 Protein-protein interactions play an essential role in almost any biological processes. Therefore, there is a particular need for methods which describe the interactions of a defined target protein in its physiological context. Here we report a method to
Michael Berg +4 more
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The non-canonical CTD of RNAP-II is essential for productive RNA synthesis in Trypanosoma brucei. [PDF]
PLoS ONE, 2009 The carboxy-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II (RNAP-II) is essential for gene expression in metazoa and yeast. The canonical CTD is characterized by heptapeptide repeats.
Anish Das, Vivian Bellofatto
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STAR Protocols, 2023 Summary: Bioorthogonal labeling and click chemistry techniques allow the detailed examination of cellular physiology through tagging and visualizing newly synthesized proteins.
Alison Keolani Carlisle +2 more
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Using Genetic Code Expansion for Protein Biochemical Studies
Frontiers in Bioengineering and Biotechnology, 2020 Protein identification has gone beyond simply using protein/peptide tags and labeling canonical amino acids. Genetic code expansion has allowed residue- or site-specific incorporation of non-canonical amino acids into proteins. By taking advantage of the
Christina Z. Chung +3 more
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A mutation in amino acid permease AAP6 reduces the amino acid content of the Arabidopsis sieve elements but leaves aphid herbivores unaffected [PDF]
, 2010 The aim of this study was to investigate the role of the amino acid permease gene AAP6 in regulating phloem amino acid composition and then to determine the effects of this altered diet on aphid performance.
Newbury, H. J. +6 more
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Nature Communications, 2023 The 20 proteinogenic amino acids cannot always satisfy the requirements of protein engineering. Here, the authors practice semi-rational non-canonical amino acid mutagenesis for a cytochrome P450 enzyme, leading to unnatural activities and significant ...
Yunjun Pan +13 more
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Incorporation of Non-Canonical Amino Acids [PDF]
, 2015 In this chapter we discuss the strengths, caveats and technical considerations of three approaches for reprogramming the chemical composition of selected amino acids within a membrane protein. In vivo nonsense suppression in the Xenopus laevis oocyte, evolved orthogonal tRNA and aminoacyl-tRNA synthetase pairs and protein ligation for biochemical ...
Lilia, Leisle +3 more
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