Results 31 to 40 of about 12,851,211 (266)

A New L-Proline Amide Hydrolase with Potential Application within the Amidase Process

Crystals, 2021
L-proline amide hydrolase (PAH, EC 3.5.1.101) is a barely described enzyme belonging to the peptidase S33 family, and is highly similar to prolyl aminopeptidases (PAP, EC. 3.4.11.5).
Sergio Martinez-Rodríguez, Rafael Contreras-Montoya, Jesús M. Torres, Luis Álvarez de Cienfuegos, Jose Antonio Gavira   +4 more
doaj   +1 more source

A non-canonical sensing pathway mediates Plasmodium adaptation to amino acid deficiency

Communications Biology, 2023
The nek4, eIK1, and eIK2 sensor kinases play a key role in Plasmodium amino acid sensing, enabling these parasites to fine-tune replication and development in response to amino acid availability.
Inês M. Marreiros, Sofia Marques, Ana Parreira, Vincent Mastrodomenico, Bryan C. Mounce, Chantal T. Harris, Björn F. Kafsack, Oliver Billker, Vanessa Zuzarte-Luís, Maria M. Mota   +9 more
doaj   +1 more source

Differences in amino acid composition of carrot α-tubulin potentially confer the resistance to dinitroaniline herbicides

Фактори експериментальної еволюції організмів, 2023
Aim. To reveal the features of amino acid composition of carrot α-tubulin isotypes that potentially determine natural tolerance to dinitroaniline herbicides. Methods. Literature and database search.
O. G. Melnyk, R. Y. Blume, P. A. Karpov
doaj   +1 more source

Initiation of Protein Synthesis with Non‐Canonical Amino Acids In Vivo

Angewandte Chemie International Edition, 2020
AbstractBy transplanting identity elements into E. coli tRNAfMet, we have engineered an orthogonal initiator tRNA (itRNATy2) that is a substrate for Methanocaldococcus jannaschii TyrRS. We demonstrate that itRNATy2 can initiate translation in vivo with aromatic non‐canonical amino acids (ncAAs) bearing diverse sidechains.
Jeffery M. Tharp, Omer Ad, Kazuaki Amikura, Fred R. Ward, Emma M. Garcia, Jamie H. D. Cate, Alanna Schepartz, Dieter Söll   +7 more
openaire   +6 more sources

Recombinant Macrocyclic Lanthipeptides Incorporating Non-Canonical Amino Acids [PDF]

Journal of the American Chemical Society, 2017
Nisin is a complex lanthipeptide that has broad spectrum antibacterial activity. In efforts to broaden the structural diversity of this ribosomally synthesized lantibiotic, we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids (ncAAs) at discrete positions.
Claudio Zambaldo, Xiaozhou Luo, Angad P. Mehta, Peter G. Schultz   +3 more
openaire   +2 more sources

Non-canonical amino acids as a tool for the thermal stabilization of enzymes

Protein Engineering, Design and Selection, 2023
AbstractBiocatalysis has become a powerful alternative for green chemistry. Expanding the range of amino acids used in protein biosynthesis can improve industrially appealing properties such as enantioselectivity, activity and stability. This review will specifically delve into the thermal stability improvements that non-canonical amino acids (ncAAs ...
Lugtenburg, Tim, Gran-Scheuch, Alejandro, Drienovská, Ivana   +2 more
openaire   +3 more sources

Click Chemistry (CuAAC) and Detection of Tagged de novo Synthesized Proteins in Drosophila

Bio-Protocol, 2019
Copper-catalyzed azide-alkyne-cycloaddition (CuAAC), also known as ‘click chemistry’ serves as a technique for bio-orthogonal, that is, bio-compatible labeling of macromolecules including proteins or lipids.
Kathrin Marter, Oliver Kobler, Ines Erdmann, Elaheh Soleimanpour, Peter Landgraf, Anke Müller, Julia Abele, Ulrich Thomas, Daniela Dieterich   +8 more
doaj   +1 more source

Facilitating the structural characterisation of non-canonical amino acids in biomolecular NMR [PDF]

Magnetic Resonance, 2022
Abstract. Peptides and proteins containing non-canonical amino acids (ncAAs) are a large and important class of biopolymers. They include non-ribosomally synthesised peptides, post-translationally modified proteins, expressed or synthesised proteins containing unnatural amino acids, and peptides and proteins that are chemically modified.
S. Kuschert, M. Stroet, Y. K.-Y. Chin, A. C. Conibear, X. Jia, T. Lee, C. R. O. Bartling, K. Strømgaard, P. Güntert, P. Güntert, P. Güntert, K. J. Rosengren, A. E. Mark, M. Mobli   +13 more
openaire   +8 more sources

β11-12 linker isomerization governs acid-sensing ion channel desensitization and recovery

eLife, 2020
Acid-sensing ion channels (ASICs) are neuronal sodium-selective channels activated by reductions in extracellular pH. Structures of the three presumptive functional states, high-pH resting, low-pH desensitized, and toxin-stabilized open, have all been ...
Matthew L Rook, Abby Williamson, John D Lueck, Maria Musgaard, David M Maclean   +4 more
doaj   +1 more source

ProRepeat: an integrated repository for studying amino acid tandem repeats in proteins [PDF]

, 2011
ProRepeat (http://prorepeat.bioinformatics.nl/) is an integrated curated repository and analysis platform for in-depth research on the biological characteristics of amino acid tandem repeats.
Harm Nijveen, Lin, K., Jack A. M. Leunissen, K. Lin, Audrey David, Luo, H., Ke Lin, A. David, David, A., H. Nijveen, H. Luo, Leunissen, J.A.M., Nijveen, H., Hong Luo, J. A. M. Leunissen   +14 more
core   +1 more source