Results 51 to 60 of about 12,851,211 (266)

In Vivo Incorporation of Multiple Unnatural Amino Acids [PDF]

, 2009
Unnatural amino acid (UAA) incorporation is an invaluable technique that is seeing increased use. THG73 is an amber suppressor tRNA used to incorporate > 100 residues at the UAG, amber stop codon, in Xenopus oocytes.
Rodriguez, Erik Ali
core   +1 more source

Extensive breaking of genetic code degeneracy with non-canonical amino acids

Nature Communications, 2023
AbstractGenetic code expansion (GCE) offers many exciting opportunities for the creation of synthetic organisms and for drug discovery methods that utilize in vitro translation. One type of GCE, sense codon reassignment (SCR), focuses on breaking the degeneracy of the 61 sense codons which encode for only 20 amino acids.
Clinton A. L. McFeely, Bipasana Shakya, Chelsea A. Makovsky, Aidan K. Haney, T. Ashton Cropp, Matthew C. T. Hartman   +5 more
openaire   +3 more sources

Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

Molecules, 2014
In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code.
Ying Ma, Hernán Biava, Roberto Contestabile, Nediljko Budisa, Martino Luigi di Salvo   +4 more
doaj   +1 more source

Leucyl-tRNA Synthetase Activates Vps34 in Amino Acid-Sensing mTORC1 Signaling

Cell Reports, 2016
Amino acid availability activates signaling by the mammalian target of rapamycin (mTOR) complex 1, mTORC1, a master regulator of cell growth. The class III PI-3-kinase Vps34 mediates amino acid signaling to mTORC1 by regulating lysosomal translocation ...
Mee-Sup Yoon, Kook Son, Edwin Arauz, Jung Min Han, Sunghoon Kim, Jie Chen   +5 more
doaj   +1 more source

The AAP gene family for amino acid permeases contributes to development of the cyst nematode Heterodera schachtii in roots of Arabidopsis [PDF]

, 2013
The beet cyst nematode Heterodera schachtii is able to infect Arabidopsis plants and induce feeding sites in the root. These syncytia are the only source of nutrients for the nematodes throughout their life and are a nutrient sink for the host plant.
Elashry, Abdelnaser, Siddique, Shahid, Koch, Wolfgang, Kreil, David, Okumoto, Sakiko, Bohlmann, Holger, Kreil, David P.   +6 more
core   +1 more source

Teaching old NCATs new tricks: using non-canonical amino acid tagging to study neuronal plasticity [PDF]

, 2013
The non-canonical amino acid labeling techniques BONCAT (bioorthogonal non-canonical amino acid tagging) and FUNCAT (fluorescent non-canonical amino acid tagging) enable the specific identification and visualization of newly synthesized proteins ...
Dieterich, D., Schuman, E., Hinz, F.
core   +2 more sources

Amino acid transporter genes are essential for FLO11-dependent and FLO11-independent biofilm formation and invasive growth in saccharomyces cerevisiae [PDF]

, 2012
Amino acids can induce yeast cell adhesion but how amino acids are sensed and signal the modulation of the FLO adhesion genes is not clear. We discovered that the budding yeast Saccharomyces cerevisiae CEN.PK evolved invasive growth ability under ...
Eckhard Boles, Sefa Alizadeh, Eckhard Boles (148580), Henrik Devitt Møller (148562), Møller, Henrik Devitt, Doreen Ochmann (148576), Møller, Henrik D., Gresham, David, Ochmann, Doreen, Doreen Ochmann, Torbensen, Rasmus, Birgitte Regenberg, Rasmus Torbensen, David Gresham (148568), Boles, Eckhard, Regenberg, Birgitte, Henrik Devitt Møller, Birgitte Regenberg (24433), Rasmus Torbensen (148554), Sefa Alizadeh (148573), Alizadeh, Sefa, David Gresham   +21 more
core   +2 more sources

Conformational plasticity of NaK2K and TREK2 potassium channel selectivity filters

Nature Communications, 2023
The potassium channel selectivity filter is responsible for conduction and selectivity of K + over other cations. Here, the authors use a combination of single molecule FRET, non-canonical fluorescent amino acid incorporation, and single channel patch ...
Marcos Matamoros, Xue Wen Ng, Joshua B. Brettmann, David W. Piston, Colin G. Nichols   +4 more
doaj   +1 more source

Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro

Nature Communications, 2020
Backbone extended monomers are poorly compatible with the natural ribosomes, impeding their polymerization into polypeptides. Here the authors design non-canonical amino acid analogs with cyclic structures or extended carbon chains and used an engineered
Joongoo Lee, Kevin J. Schwarz, Do Soon Kim, Jeffrey S. Moore, Michael C. Jewett   +4 more
doaj   +1 more source

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source