Harnessing Transaminases to Construct Azacyclic Non-Canonical Amino Acids [PDF]
Nature Synthesis, 2023 Non-canonical amino acids (ncAAs) are prized building blocks in the synthesis of natural products, designer peptides and drug molecules. Despite their general utility, the complex structure of these molecules still presents an enormous challenge for ...
Hans, Renata +4 more
core +3 more sources
Metabolic Implications of Using BioOrthogonal Non-Canonical Amino Acid Tagging (BONCAT) for Tracking Protein Synthesis [PDF]
Frontiers in Microbiology, 2020 BioOrthogonal Non-Canonical Amino acid Tagging (BONCAT) is a powerful tool for tracking protein synthesis on the level of single cells within communities and whole organisms.
Katherine F. Steward +14 more
doaj +2 more sources
Bioorthogonal non-canonical amino acid tagging reveals translationally active subpopulations of the cystic fibrosis lung microbiota [PDF]
Nature Communications, 2020 Bioorthogonal non-canonical amino acid tagging (BONCAT) coupled with flow cytometry (FACS) has been utilized to profile active microbiome of environmental samples.
Talia D. Valentini +6 more
doaj +2 more sources
Cell-specific non-canonical amino acid labelling identifies changes in the de novo proteome during memory formation [PDF]
eLife, 2020 The formation of spatial long-term memory (LTM) requires the de novo synthesis of distinct sets of proteins; however, a non-biased examination of the de novo proteome in this process is lacking. Here, we generated a novel mouse strain, which enables cell-
Harrison Tudor Evans +2 more
doaj +2 more sources
Overview on Multienzymatic Cascades for the Production of Non-canonical α-Amino Acids [PDF]
Frontiers in Bioengineering and Biotechnology, 2020 The 22 genetically encoded amino acids (AAs) present in proteins (the 20 standard AAs together with selenocysteine and pyrrolysine), are commonly referred as proteinogenic AAs in the literature due to their appearance in ribosome-synthetized polypeptides.
Sergio Martínez-Rodríguez +3 more
doaj +4 more sources
Non-canonical amino acids in protein engineering
Current Opinion in Biotechnology, 2003 Methods for engineering proteins that contain non-canonical amino acids have advanced rapidly in the past few years. Novel amino acids can be introduced into recombinant proteins in either a residue-specific or site-specific fashion.
Mock, Marissa L. +2 more
core +4 more sources
A computational approach for designing D-proteins with non-canonical amino acid optimised binding affinity. [PDF]
PLoS ONE, 2017 Redesigning protein surface topology to improve target binding holds great promise in the search for highly selective therapeutics. While significant binding improvements can be achieved using natural amino acids, the introduction of non-canonical ...
Michael Garton +2 more
doaj +2 more sources
Non-canonical Amino Acid Substrates of E. coli Aminoacyl-tRNA Synthetases. [PDF]
Chembiochem, 2022 Hartman MCT.
europepmc +2 more sources
Mechanistic studies of non-canonical amino acid mutagenesis. [PDF]
Methods Enzymol, 2021 Abstract Over the past decade, harnessing the cellular protein synthesis machinery to incorporate non-canonical amino acids (ncAAs) into tailor-made peptides has significantly advanced many aspects of molecular science. More recently, groundbreaking progress in our ability to engineer this machinery for improved ncAA incorporation has ...
Fleisher RC, Michael N, Gonzalez RL.
europepmc +4 more sources
Cell ReportsSummary: Neuronal response to changes in nutrient availability is critical for maintaining metabolic homeostasis and organismal survival. Nevertheless, we know little about the molecular players that regulate and maintain neurotransmission under ...
Megumi Mori +5 more
doaj +2 more sources