Results 121 to 130 of about 10,612,087 (294)

Genome-scale metabolic models reveal determinants of phenotypic differences in non-Saccharomyces yeasts. [PDF]

BMC Bioinformatics, 2023
Pettersen JP, Castillo S, Jouhten P, Almaas E.   +3 more
europepmc   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Alternative sources of polysaccharides in oenology: the role of non-Saccharomyces yeasts in wine quality

openLa presente tesi approfondisce il ruolo dei lieviti non appartenenti al genere Saccharomyces nella biosintesi di polisaccaridi, sostanze che influenzano attivamente la qualità organolettica e strutturale del vino. Questo ambito di studio rappresenta
ZECCHIN, SIMONE
core  

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

Cytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson, Meghan E. O'Connell, Kaitlin E. Homa, Cristian Suarez, Michael L. James, Kashmeera D. Baboolall, Vilmos Zsolnay, Vladimir Sirotkin, David R. Kovar   +8 more
wiley   +1 more source

Application of Non-Saccharomyces Yeasts in Co-Inoculation: Impact of Metschnikowia pulcherrima and Lachancea thermotolerans on Syrah's Acidity and Aromatic Profile

reservedI lieviti non-Saccharomyces sono stati a lungo considerati microrganismi dannosi o poco significativi dal punto di vista fermentativo, in quanto spesso associati a un’elevata produzione di acidità volatile e sentori negativi, associati poi ad ...
FERRARO, LUCA
core  

Microtubule Destabilizing Kinesins: A Historical Perspective and Thoughts for the Future

Cytoskeleton, EarlyView.
ABSTRACT The microtubule cytoskeleton plays critical roles in multiple cellular processes such as mitotic spindle assembly and chromosome segregation. Cells contain a number of microtubule‐associated proteins that regulate microtubule dynamics both temporally and spatially in cells. The microtubule depolymerizing kinesins include members of the Kinesin‐
Claire E. Walczak
wiley   +1 more source

Les levures non-Saccharomyces (dynamique, caractérisation et interaction avec Saccharomyces durant les étapes pré-fermentaires et la fermentation alcoolique)

, 2008
Cette thèse se rapporte à l'étude des levures présentes dans la niche écologique raisin-vin . La dynamique et l'identité des levures non-Saccharomyces pendant la macération pré-fermentaire et pendant la fermentation alcoolique dans les moûts du Bordelais
MASNEUF, Isabelle, ZOTT, Katharina
core  

Quantifying Actin Persistence Length in S. pombe and S. cerevisiae: The Impact of Data Size, Filament Length and Fitting Strategy

Cytoskeleton, EarlyView.
ABSTRACT Actin filaments exhibit isoform and species‐specific mechanical properties that are commonly quantified via their persistence length, yet the reliability of such measurements depends not only on the analysis method but also on dataset characteristics.
Peter Nietmann, Andrejus Suchenko, Andreas Janshoff, Mohan K. Balasubramanian   +3 more
wiley   +1 more source

Benchmarking of Fluorescent Proteins in Trichoderma reesei Using Flow Cytometry

Cytometry Part A, EarlyView.
ABSTRACT Fungi are the subject of extensive research due to their significant impact on human life. However, the development of tools based on fluorescent proteins has been slow in these species. Trichoderma reesei, a filamentous fungus known for its high secretion capability, shows great potential for producing heterologous proteins.
Métivier Killian, Bidard Frédérique, Jourdier Etienne, Mathis Hugues   +3 more
wiley   +1 more source