Results 131 to 140 of about 10,228,992 (308)

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Application of Non-Saccharomyces Yeasts in Co-Inoculation: Impact of Metschnikowia pulcherrima and Lachancea thermotolerans on Syrah's Acidity and Aromatic Profile

reservedI lieviti non-Saccharomyces sono stati a lungo considerati microrganismi dannosi o poco significativi dal punto di vista fermentativo, in quanto spesso associati a un’elevata produzione di acidità volatile e sentori negativi, associati poi ad ...
FERRARO, LUCA
core  

Három Saccharomyces élesztő törzs keresztezéséből származó mesterséges kimérák molekuláris genetikai vizsgálata

, 2018
A laboratóriumokban mesterségesen létrehozott kimérák vizsgálata során, újabb ismereteket kaphatunk az interspecifikus hibridekről. Három Saccharomyces faj keresztezéséből származó kimérák kromoszóma készleteinek a vizsgálatáról szól a szakdolgozat ...
Hostyisóczki, Ákos
core  

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

Cytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson, Meghan E. O'Connell, Kaitlin E. Homa, Cristian Suarez, Michael L. James, Kashmeera D. Baboolall, Vilmos Zsolnay, Vladimir Sirotkin, David R. Kovar   +8 more
wiley   +1 more source

Use of non-Saccharomyces yeasts and oenological tannin in red winemaking: Influence on colour, aroma and sensorial properties of young wines

, 2018
Today, many non-Saccharomyces strains have been verified can be positive for the development of wine anthocyanin and aroma in different fermentation scenarios.
Calderon, Fernando, Suárez-Lepe, Jose Antonio, Han, Shunyu, Juan Manuel del Fresno, Morata, Antonio, Santiago Benito, Escott, Carlos, Loira, Iris, Fernando Calderon, Shunyu Han, Wendu Tesfaye, Benito, Santiago, Carlos Escott, Antonio Morata, Iris Loira, Chen, Kai, del Fresno, Juan Manuel, Jose Antonio Suárez-Lepe, Kai Chen   +18 more
core   +1 more source

Microtubule Destabilizing Kinesins: A Historical Perspective and Thoughts for the Future

Cytoskeleton, EarlyView.
ABSTRACT The microtubule cytoskeleton plays critical roles in multiple cellular processes such as mitotic spindle assembly and chromosome segregation. Cells contain a number of microtubule‐associated proteins that regulate microtubule dynamics both temporally and spatially in cells. The microtubule depolymerizing kinesins include members of the Kinesin‐
Claire E. Walczak
wiley   +1 more source

Secretopathies emerge as a new class of neurocristopathies

Developmental Dynamics, EarlyView.
Abstract Neural crest cells are a transient embryonic population of cells that give rise to a wide range of structures, including craniofacial cartilage and bone, peripheral neurons and glia, as well as components of the cardiac outflow tract, among others.
Amanda Teixeira, Cassandra Millet‐Boureima, Joshua A. Moore, Loydie A. Jerome‐Majewska   +3 more
wiley   +1 more source

Saccharomyces izolátumok patogenitásának és humán makrofágokkal történő interakcióinak vizsgálata

, 2016
A diplomamunka magában foglalja különböző Saccharomyces izolátumok genotipizálását, patogenitásának vizsgálatát, illetve immunstimuláló hatását, melyet humán makrofágokkal történő interakció során vizsgáltunk.gjBiotechnológiaMSc ...
Pintér, Lilla
core  

Exploiting Biocatalysis for the Scalable Synthesis of Enantiopure γ‐Hydroxynitrile Building Blocks

European Journal of Organic Chemistry, EarlyView.
Screening a panel of (S)‐and (R)‐selective alcohol dehydrogenases identified several biocatalysts for stereoselectively reducing γ‐ketonitriles into γ‐hydroxynitriles. These processes were optimized, delivering the targets in yields up to 88% with e.e.’s up to 99%. Scalability of the methodology was achieved to produce gram quantities of product, which
Benjamin Baumert, Kathryn Yeow, Roi Boris Tchuemmogne Tchuente, Adam Cruise, Christopher Thomas, Thomas Bayer, Uwe T. Bornscheuer, Marcus Baumann   +7 more
wiley   +1 more source