Results 151 to 160 of about 42,828,722 (241)

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

An Improved Dengue Virus Serotype-Specific Non-Structural Protein 1 Capture Immunochromatography Method with Reduced Sample Volume. [PDF]

Biosensors (Basel)
Sretapunya W, Buranachat T, Prasomthong M, Tantikorn R, Sa-Ngarsang A, Naemkhunthot S, Meephaendee L, Wongjaroen P, Tanaka C, Shimadzu Y, Ogata K, Kaihatsu K, Morita R, Shirano M, Phadungsombat J, Sasaki T, Kubota-Koketsu R, Samune Y, Nakayama EE, Shioda T.   +19 more
europepmc   +1 more source

Identification of potential drugs against SARS-CoV-2 non-structural protein 1 (nsp1). [PDF]

J Biomol Struct Dyn, 2021
de Lima Menezes G, da Silva RA.
europepmc   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Glycans on non-structural protein 1 prevent premature T-cell mediated dengue virus clearance. [PDF]

EMBO Mol Med
Idris F, Ooi JSG, Ting DHR, Tan ETX, Wan C, Benke PI, Marzinek JK, Copping JM, Li QH, Ng LY, Lim SY, Walsh I, Allison JR, Bond PJ, Torta F, Nguyen-Khuong T, Chan KR, Alonso S.   +17 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

An evolutionarily conserved strategy for ribosome binding and inhibition by β-coronavirus non-structural protein 1

, 2023
Maurina SF, O’Sullivan JP, Sharma G, Pineda Rodriguez DC, MacFadden A, Cendali F, Henen MA, Kieft JS, Glasgow A, Steckelberg A.   +9 more
europepmc   +1 more source

Cross-reactive antibodies targeting surface-exposed non-structural protein 1 (NS1) of dengue virus-infected cells recognize epitopes on the spaghetti loop of the β-ladder domain. [PDF]

PLoS One, 2022
Kraivong R, Traewachiwiphak S, Nilchan N, Tangthawornchaikul N, Pornmun N, Poraha R, Sriruksa K, Limpitikul W, Avirutnan P, Malasit P, Puttikhunt C.   +10 more
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Viral-Host Interactome Analysis Reveals Chicken STAU2 Interacts With Non-structural Protein 1 and Promotes the Replication of H5N1 Avian Influenza Virus. [PDF]

Front Immunol, 2021
Wang Q, Zhang Q, Zheng M, Wen J, Li Q, Zhao G.   +5 more
europepmc   +1 more source