Results 71 to 80 of about 11,612,374 (304)

Versatile vector tools for efficient protein screening across multiple expression systems

FEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu, Yaqing Liu, Lei Qin, Feng Yu, Qisheng Wang, Yahui Liu   +5 more
wiley   +1 more source

Loss of AMBRA1 activates MAPK and angiogenesis signaling pathways in melanoma cells

FEBS Open Bio, EarlyView.
Loss of AMBRA1 in melanoma cells activates multiple oncogenic pathways associated with tumor progression. Transcriptomic and protein network analyses revealed that AMBRA1 depletion enhances MAPK/ERK signaling, angiogenesis, TGF‐β/EMT signaling, and Wnt/axon guidance pathways.
Milad Ibrahim, Marco Corazzari, Iman Osman, Jane Armstrong, Noel Carter   +4 more
wiley   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

Current status of recombinant duck enteritis virus vector vaccine research

Frontiers in Veterinary Science
Duck enteritis virus (DEV), the pathogen of duck viral enteritis, belongs to the α-herpesvirus subfamily. Like other herpesviruses, it has a large genome with multiple non-coding and non-essential regions for viral replication.
Wen-Feng Jia, An-Ping Wang, Zhi Wu, Xin-Nuo Lei, Yu-Ting Cheng, Shan-Yuan Zhu   +5 more
doaj   +1 more source

Antiviral RNA Interference Activity in Cells of the Predatory Mosquito, Toxorhynchites amboinensis

Viruses, 2018
Arthropod vectors control the replication of arboviruses through their innate antiviral immune responses. In particular, the RNA interference (RNAi) pathways are of notable significance for the control of viral infections.
Claire L. Donald, Margus Varjak, Eric Roberto Guimarães Rocha Aguiar, João T. Marques, Vattipally B. Sreenu, Esther Schnettler, Alain Kohl   +6 more
doaj   +1 more source

Focus on Cell Biology of Virus-Plant and Virus-Vector Interactions

Molecular Plant-Microbe Interactions, 2020
A successful viral infection requires complex, compatible molecular interactions between the invading virus and the host. A better understanding of such interactions may assist in the development of novel approaches to control viral diseases for ...
Aiming Wang, Tessa M Burch-Smith, Yi Li
doaj   +1 more source

Development and application of non-integrating lentiviral vectors for gene therapy [PDF]

, 2009
Lentiviruses stably integrate their genome into the host genome. Although this feature can be advantageous for long term transgene expression, it also has the potential to cause mutagenesis and cell transformation.
Apolonia, L.F.S.
core  

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

pEPito: a significantly improved non-viral episomal expression vector for mammalian cells

BMC Biotechnology, 2010
Background The episomal replication of the prototype vector pEPI-1 depends on a transcription unit starting from the constitutively expressed Cytomegalovirus immediate early promoter (CMV-IEP) and directed into a 2000 bp long matrix attachment region ...
Ogris Manfred, Lipps Hans J, Harbottle Richard P, Wong Suet-Ping, Argyros Orestis, Haase Rudolf, Magnusson Terese, Pinto Maria, Haas Jürgen, Baiker Armin   +9 more
doaj   +1 more source

Amino Acid-Linked Low Molecular Weight Polyethylenimine for Improved Gene Delivery and Biocompatibility

Molecules, 2020
The construction of efficient and low toxic non-viral gene delivery vectors is of great significance for gene therapy. Herein, two novel polycations were constructed via Michael addition from low molecular weight polyethylenimine (PEI) 600 Da and amino ...
Xiao-Ru Wu, Ji Zhang, Ju-Hui Zhang, Ya-Ping Xiao, Xi He, Yan-Hong Liu, Xiao-Qi Yu   +6 more
doaj   +1 more source