Results 81 to 90 of about 11,612,374 (304)

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

A viral movement protein mediates host volatile biosynthesis to co-attract vectors and non-vectors and enhances viral infection

Frontiers in Plant Science
The literature shows that vector-transmitted plant viruses mediate host volatile biosynthesis to attract vectors and repel non-vectors, benefiting plant-infecting viruses and enhancing their spread.
Shijun Li, Xiangwen Luo, Xiangwen Luo, Xiangwen Luo, Yu Zhang, Yu Zhang, Zhanhong Zhang, Zhanhong Zhang, Zhanhong Zhang, Xiao Yang, Xiao Yang, Xuguo Zhou, Xin Wang, Xian OuYang, Xian OuYang, Xiaoxin Qu, Deyong Zhang, Deyong Zhang, Deyong Zhang, Deyong Zhang, Songbai Zhang, Songbai Zhang, Songbai Zhang, Songbai Zhang, Yong Liu, Yong Liu, Yong Liu, Yong Liu   +27 more
doaj   +1 more source

Factors influencing in vivo transduction by recombinant adeno-associated viral vectors expressing the human factor IX cDNA. [PDF]

, 2001
Long-term expression of coagulation factor IX (FIX) has been observed in murine and canine models following administration of recombinant adeno-associated viral (rAAV) vectors into either the portal vein or muscle. These studies were designed to evaluate
Hanawa, H, Vanin, EF, Nathwani, AC, Zhou, JF, Davidoff, A, Nienhuis, AW   +5 more
core  

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source

Non-viral nucleic acid delivery methods

, 2017
Introduction: Delivery of nucleic acid-based molecules in human cells is a highly studied approach for the treatment of several disorders including monogenic diseases and cancers.
David Guay (3384599), Mathias Mangion (3384593), Bruno Gaillet (3384605), Igor Slivac (3384602), Juliette Champeil (3384596)   +4 more
core   +1 more source

TRPML1 agonist ML‐SA5 attenuates pulmonary fibroblast activation by suppressing mTOR and restoring autophagic flux

FEBS Open Bio, EarlyView.
TGF‐β1 stimulation downregulates lysosomal channel TRPML1 in pulmonary fibroblasts. The TRPML1 agonist ML‐SA5 suppressed fibroblast‐to‐myofibroblast activation and collagen production. Mechanistically, ML‐SA5 inhibited mTOR phosphorylation, restored autophagic flux, and its effects were enhanced by rapamycin (mTOR inhibitor) and reversed by MHY1485 ...
Jiatong Yao, Xiaoting Kang, Lulu Meng, Shaoxuan Zhang, Qiujie Li, Jinyu Zhou, Mengyue Xue, Chu Li, Hua Zhu, Pengzhou Hang, Jing Zhao   +10 more
wiley   +1 more source

Parameters Affecting Image-guided, Hydrodynamic Gene Delivery to Swine Liver

Molecular Therapy: Nucleic Acids, 2013
Development of a safe and effective method for gene delivery to hepatocytes is a critical step toward gene therapy for liver diseases. Here, we assessed the parameters for gene delivery to the livers of large animals (pigs, 40–65 kg) using an image ...
Kenya Kamimura, Takeshi Suda, Guisheng Zhang, Yutaka Aoyagi, Dexi Liu   +4 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Early Clinical and Cerebrospinal Fluid Predictors of 1‐Year Recurrence in Autoimmune GFAP Astrocytopathy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Autoimmune glial fibrillary acidic protein astrocytopathy (GFAP‐A) is an inflammatory central nervous system disorder with variable outcomes. Relapse occurs in a subset of patients, but early predictors remain unclear. We aimed to identify admission‐available features associated with 1‐year recurrence and develop an interpretable ...
Qingting Hong, Yuping Xiao, Yuhan Wu, Ziyuan Huang, Weishan Liang, Li Xiao, Yaqing Shu, Tingting Lu, Yanyu Chang, Wei Qiu, Yuge Wang   +10 more
wiley   +1 more source

Limitations of Clinical Application of siRNA Delivery Based on Non-Viral Vectors

مجله دانشکده پزشکی اصفهان, 2014
In recent years, much more attentions have been focused on siRNA-based gene therapy. This approach is based on PTGS (Post-transcriptional gene silencing).
Reza Ghavimi, Meraj Pourhossein
doaj