Zooming in: PAGE-Northern Blot Helps to Analyze Anti-Sense Transcripts Originating from Human rIGS under Transcriptional Stress [PDF]
Non-Coding RNA, 2021 Ribosomal intergenic spacer (rIGS), located between the 45S rRNA coding arrays in humans, is a deep, unexplored source of small and long non-coding RNA molecules transcribed in certain conditions to help a cell generate a stress response, pass through a ...
Anastasia A. Sadova +2 more
doaj +4 more sources
Ribosome Purification from an α-proteobacterium and rRNA Analysis by Northern Blot [PDF]
Bio-Protocol, 2020 Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades.
Liang Yin, Caroline Harwood
doaj +4 more sources
Preparation of monoclonal antibody against human KIAA0100 protein and Northern blot analysis of human KIAA0100 gene [PDF]
Journal of Pharmaceutical Analysis, 2017 Monoclonal antibodies (MAbs) are important tools for the study of proteins′ function and structure. But there has been no report on the preparation of MAbs against human KIAA0100 protein up to date.
He Cui +4 more
doaj +3 more sources
High-Resolution Northern Blot for a Reliable Analysis of MicroRNAs and Their Precursors [PDF]
The Scientific World Journal, 2011 This protocol describes how to perform northern blot analyses to detect microRNAs and their precursors with single-nucleotide resolution, which is crucial for analyzing individual length variants and for evaluating relative quantities of unique microRNAs
Edyta Koscianska +3 more
doaj +4 more sources
Methods Enzymol, 2013 To measure the size and amount of RNA transcribed from a specific gene of interest.
He SL, Green R.
europepmc +5 more sources
Modified Northern blot protocol for easy detection of mRNAs in total RNA using radiolabeled probes [PDF]
BMC Genomics, 2022 Background Northern blotting is still used as a gold standard for validation of the data obtained from high-throughput whole transcriptome-based methods.
Tao Yang, Mingdi Zhang, Nianhui Zhang
doaj +2 more sources
Northern Blot of tRNA in Yeast [PDF]
Bio-Protocol, 2013 tRNAs are small RNAs around 70-90 nt. tRNAs are different from many other small RNAs in that they are very abundant, which makes it difficult to study their transcriptional regulation by traditional northern blot.
Yuehua Wei
doaj +2 more sources
Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation [PDF]
Bio-Protocol, 2019 Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern).
Christopher Fields +4 more
doaj +2 more sources
Quantification of Queuosine Modification Levels in tRNA from Human Cells Using APB Gel and Northern Blot [PDF]
Bio-Protocol, 2019 Queuosine (Q) is a hypermodified base in the wobble anticodon position of tRNAs coding for the amino acids Tyr, His, Asn, and Asp. tRNA Q-modification is introduced by a queuine tRNA-ribosyltransferase (TGT) that replaces the guanine base at G34 at these
Zaneta Matuszek, Tao Pan
doaj +2 more sources
Quantitative Expression Analysis in Brassica napus by Northern Blot Analysis and Reverse Transcription-Quantitative PCR in a Complex Experimental Setting. [PDF]
PLoS ONE, 2016 Analysis of gene expression is one of the major ways to better understand plant reactions to changes in environmental conditions. The comparison of many different factors influencing plant growth challenges the gene expression analysis for specific gene ...
Annekathrin Rumlow +6 more
doaj +2 more sources