Results 1 to 10 of about 57,891 (288)

Northern Blotting [PDF]

, 2013
To measure the size and amount of RNA transcribed from a specific gene of interest.
Shan L, He, Rachel, Green
core   +7 more sources

Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments [PDF]

STAR Protocols
Summary: Transfer RNA (tRNA), its post-transcriptional modifications, and tRNA-derived fragments (tRFs) play essential roles in cellular processes and gene regulation.
Pavlina Gregorova, Minna-Maria K. Heinonen, Milla M. Laarne, L. Peter Sarin   +3 more
doaj   +2 more sources

Validation and characterization of murine gammaherpesvirus 68 antisense transcripts by northern blot analysis and quantitative reverse transcription-PCR [PDF]

Archives of Biological Sciences, 2023
The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different RNA species, including long non-coding RNAs (
Kara Mehmet
doaj   +1 more source

Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. [PDF]

PLoS ONE, 2015
The biological roles of RNA modifications are still largely not understood. Thus, developing a method for detecting RNA modifications is important for further clarification.
Eikan Mishima, Daisuke Jinno, Yasutoshi Akiyama, Kunihiko Itoh, Shinnosuke Nankumo, Hisato Shima, Koichi Kikuchi, Yoichi Takeuchi, Alaa Elkordy, Takehiro Suzuki, Kuniyasu Niizuma, Sadayoshi Ito, Yoshihisa Tomioka, Takaaki Abe   +13 more
doaj   +1 more source

A Comprehensive Analysis of Northern versus Liquid Hybridization Assays for mRNAs, Small RNAs, and miRNAs Using a Non-Radiolabeled Approach

Current Issues in Molecular Biology, 2021
Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad, Bushra Gull, Jasmin Baby, Farah Mustafa   +3 more
doaj   +1 more source

Artificial microRNA-Based RNA Interference and Specific Gene Silencing for Developing Insect Resistance in Solanum lycopersicum

Agronomy, 2021
RNA Interference (RNAi), which works against invading nucleic acids or modulates the expression of endogenous genes, is a natural eukaryotic regulating system, and it works by noncoding smaller RNA molecules.
Mohammad Faisal, Eslam M. Abdel-Salam, Abdulrahman A. Alatar   +2 more
doaj   +1 more source

Isolation and detection of small RNA molecules

Plant, Soil and Environment, 2007
The article describes in detail methodical procedures for isolation of small RNA molecules, their electrophoresis, northern blotting and finally detection by hybridization with radioactively labeled riboprobes.
J. Fulneček
doaj   +1 more source

Primary culture and mRNA analysis of human ovarian cells

Biological Procedures Online, 2002
Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer (OC) cell lines exist, however, most have acquired significant genetic alterations from their cells of origin, including ...
Dunfield Lesley D., Shepherd Trevor G., Nachtigal Mark W.   +2 more
doaj   +1 more source

Polyclonal antibodies against potato spindle tuber viroid RNA

Frontiers in Bioscience-Elite, 2022
Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with
Yuri Drygin, Tatiana Gasanova, Konstantin Butenko   +2 more
doaj   +1 more source

Northern blotting [PDF]

Proceedings of the Nutrition Society, 1996
Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by Northern blotting. A cDNA radioactively labelled with 32P is the most-commonly-used hybridization probe.
openaire   +2 more sources