Results 1 to 10 of about 1,606,720 (296)

Optimized RNA Extraction and Northern Hybridization in Streptomycetes

Biological Procedures Online, 2010
Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity and recovery and unbiased transfer of the ...
Tagliavia Marcello, Taravella Anna, Marineo Sandra, Puglia Anna, La Farina Mario   +4 more
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Identification and characteristics of microRNAs from Bombyx mori

BMC Genomics, 2008
Background MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression by targeting messenger RNAs (mRNAs) and causing mRNA cleavage or translation blockage. Of the 355 Arthropod miRNAs that have been identified, only 21 are B.
Gao Xiaolian, Zhou Songping, Sheng Qing, Lv Zhengbing, Chen Jian, Chen Jianqing, Nie Zuoming, He Ping-an, Kong Lingyin, Wu Xiangfu, Jin Yongfeng, Zhang Yaozhou   +11 more
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Polysome Profiling Analysis

Bio-Protocol, 2013
Polysome profiling is a method that allows monitoring of translation activity of mRNAs in cells and tissues. Once each polysome fractions are collected, the translation activity of each mRNA is analyzed using various molecular biology techniques such as ...
Masahiro Morita, Tommy Alain, Ivan Topisirovic, Nahum Sonenberg   +3 more
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Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting

STAR Protocols
Summary: tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N2, N2-dimethylguanosine (m22G) modifications on tRNAs using an antibody-based detection approach.
Sseu-Pei Hwang, Katherine Barondeau, Catherine Denicourt   +2 more
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Identification of a cartilage specific novel miRNA which directly targets PRMT3 in rats

Osteoarthritis and Cartilage Open, 2021
Objective: Through experiments to testify a candidate novel miRNA previously discovered by us is a real miRNA and involved in cartilage development. Design: The miR-novel and the newly hairpin miRNA transcribed sequence (pre-miR-novel) was verified as a ...
Jian Sun, Ph. D., Quancheng Wang, Ying Yuan, Safdar Hussain, Ph. D., Yitong Zhao, Yuanxu Guo, Ph. D., Mengyao Sun, Ph. D., Huang Huang, Xinyu Huo, Fujun Zhang, Qilan Ning, Yan Han, Peng Xu, M.D., Ph. D., Shemin Lu, M.D., Ph. D.   +13 more
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Differential expression of metallothionein type-2 homologues in leaves and roots of Black pepper (Piper nigrum L)

Genetics and Molecular Biology, 2008
Black pepper (Piper nigrum L.), member of the family Piperaceae is indigenous to India and is one of the most widely used spices in the world. In this paper we report the results of our attempts to identify a set of genes differentially expressed in the ...
Susan M. Alex, Jose Dicto, M.G. Purushothama, S. Manjula   +3 more
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MOLECULAR PATHOBIOLOGICAL AND SCANNING ELECTRON MICROSCOPIC CHANGES IN HORSE TENDON CELLS TREA TED WITH ENROFLOXACIN

Pakistan Veterinary Journal, 2003
Fluoroquinolone (FQNL) antibiotics have been used widely in horses because of their broad-spectrum bactericidal activity and relative safety, however, their use is not without risk.
A. Khan1 and J. Halper
doaj  

Identification and characterization of a novel group of natural anti-sense transcripts from RNA1.2 gene locus of human cytomegalovirus

Chinese Medical Journal, 2019
. Background:. Natural anti-sense transcripts (NATs), which are transcribed from the complementary DNA strand of annotated genes, exert regulatory function of gene expression.
Bing-Yang Liu, Yan-Ping Ma, Ying Qi, Yu-Jing Huang, Zhong-Yang Liu, Meng-Qing Lu, Qiang Ruan, Xin Chen   +7 more
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Stimulation of ZPC Production by Follicle-Stimulating Hormone in the Granulosa Cells of Japanese Quail (Coturnix Japonica)

The Journal of Poultry Science, 2003
Avian perivitelline membrane, an investment homologous to the zona pellucida of mammalian oocytes, is composed of two glycoproteins. Previous studies have indicated that one of the components, a glycoprotein homologous to mammalian ZPC, is produced and ...
Jianzhi Pan, Tomohiro Sasanami, Makoto Mori   +2 more
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Rapid quantitation of mRNA species in ethidium bromide-stained gels of competitive RT-PCR products.

Journal of Lipid Research, 1994
A rapid method for quantifying the low-abundant mRNAs of the low density lipoprotein receptor and the 3-hydroxy-3-methylglutaryl coenzyme A reductase by competitive polymerase chain reaction is presented.
A Gebhardt, A Peters, D Gerding, A Niendorf   +3 more
doaj   +1 more source