Results 11 to 20 of about 57,891 (288)
Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting [PDF]
STAR ProtocolsSummary: tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N2, N2-dimethylguanosine (m22G) modifications on tRNAs using an antibody-based detection approach.
Sseu-Pei Hwang +2 more
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Northern Blotting of RNA Denatured in Glyoxal Without Buffer Recirculation [PDF]
BioTechniques, 1997 A rapid and easy procedure for preparing Northern blots is described. RNA is denatured with glyoxal in the presence of ethidium bromide and glycerol, then electrophoresed through agarose in a buffer that does not require recirculation.
William V. Burnett
doaj +2 more sources
Total Bacterial RNA Isolation and Northern Blotting Analysis [PDF]
, 2023 The study of bacterial gene expression during infection provides vital information for researchers to understand bacterial pathogenesis and infection. The ability to obtain clean and undegraded RNA could be challenging and daunting and remains the most ...
Loh, E,, Karlsson, J,, Eichner, H,
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HDAC11-S4 RNA 4 was validated by Northern blotting and in situ hybridization. [PDF]
, 2023 A, Validation of HDAC11-S4 RNA 4 by Northern blotting. The total RNA extracted from the BmCPV-infected midgut was separated on 1% agarose-formaldehyde gels (left) and transferred to Hybond-N+ nylon membranes.
Chengliang Gong (299403) +7 more
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Rapid amplification of cDNA ends (RACE) and northern blotting of the 5′ UTR of the CO1 mRNA. [PDF]
, 2021 (A) RACE of the 5′ UTR of CO1 mRNA in WT (control) and FASTKDs-KO HAP1 cells. (B) Sanger sequencing of the 5′ UTR of CO1 mRNA from WT HAP1 cells. (C) RACE of the 5′ end of CO2 mRNA in WT (control) and FASTKDs-KO HAP1 cells.
Akira Ohkubo (2955393) +8 more
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Oligos for northern blotting (dsDNA probes). [PDF]
, 2013 Oligos for northern blotting (dsDNA probes).
Sara C. Cloutier (487136) +4 more
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Northen blotting with DIG Northern Starter Kit (Roche®) v1 [PDF]
, 2016 This script is used for northern blotting with the Roche DIG Northern Starter Kit.
Lutz Berwanger
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Primers used for gene cloning and northern blotting. [PDF]
, 2016 Primers used for gene cloning and northern blotting.
Toshihiro Suzuki (278774) +4 more
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Detection and Verification of Mammalian Mirtrons by Northern Blotting [PDF]
, 2018 microRNAs (miRNAs) have vital roles in regulating gene expression—contributing to major diseases like cancer and heart disease. Over the last decade, thousands of miRNAs have been discovered through high throughput sequencing-based annotation.
Alex S. Flynt +3 more
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