Results 21 to 30 of about 57,891 (288)
Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA
BioTechniques, 1998 A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht +2 more
doaj +1 more source
, 2005 The capacity to separate nucleic acids and proteins by their molecular weight and identify fractions of interest by the hybridization of specific labeled probes is known as blotting.
Hibbitts, Samantha Jayne, White, P. L.
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Figure 1D - tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by Northern blotting analysis [PDF]
, 2018 tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by northern blotting analysis. Total tRNA was extracted from cells growing in MOPS minimal medium with 0.2% glucose as the carbon source before and after isoleucine ...
Gerdes, K
core +3 more sources
Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo
Virology Journal, 2023 Background In addition to the well-known coronavirus genomes and subgenomic mRNAs, the existence of other coronavirus RNA species, which are collectively referred to as noncanonical transcripts, has been suggested; however, their biological ...
Ching-Hung Lin +10 more
doaj +1 more source
RNA Isolation and Northern Blot Analysis
Bio-Protocol, 2014 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao +5 more
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Northern blotting analysis of microRNAs, their precursors and RNA interference triggers [PDF]
, 2011 Background Numerous microRNAs (miRNAs) have heterogeneous ends resulting from imprecise cleavages by processing nucleases and from various non-templated nucleotide additions.
Paulina Galka-Marciniak +5 more
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Immuno-northern blotting using antibodies against modified nucleosides. [PDF]
, 2015 (A) Structural formulas of 1-methyladenosine (m1A), N6-methyladenosine (m6A), pseudouridine (Ψ), and 5-methylcytidine (m5C). (B) Total RNA (0.6 μg) isolated from the indicated samples was separated in the 12% polyacrylamide-8 M urea denaturing gel and ...
Kuniyasu Niizuma (830646) +13 more
core +1 more source
Frontiers in Microbiology, 2017 In bacteria, small non-coding RNAs (sRNAs) are critical regulators of cellular adaptation to changes in metabolism, physiology, or the external environment.
Xie Fuli +7 more
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Methods for the Detection of D-Amino-Acid Oxidase
Biological Procedures Online, 1998 Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
Konno Ryuichi
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melRNA-seq for Expression Analysis of SINE RNAs and Other Medium-Length Non-Coding RNAs
Mobile DNA, 2021 Background Small interspersed elements (SINEs) are transcribed by RNA polymerase III (Pol III) to produce RNAs typically 100–500 nucleotides in length.
Yoshinobu Mori, Kenji Ichiyanagi
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