Results 21 to 30 of about 1,606,720 (296)
Primary culture and mRNA analysis of human ovarian cells
Biological Procedures Online, 2002 Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer (OC) cell lines exist, however, most have acquired significant genetic alterations from their cells of origin, including ...
Dunfield Lesley D. +2 more
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Polyclonal antibodies against potato spindle tuber viroid RNA
Frontiers in Bioscience-Elite, 2022 Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with
Yuri Drygin +2 more
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Northern Blotting of RNA Denatured in Glyoxal Without Buffer Recirculation
BioTechniques, 1997 A rapid and easy procedure for preparing Northern blots is described. RNA is denatured with glyoxal in the presence of ethidium bromide and glycerol, then electrophoresed through agarose in a buffer that does not require recirculation.
William V. Burnett
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Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA
BioTechniques, 1998 A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht +2 more
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Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo
Virology Journal, 2023 Background In addition to the well-known coronavirus genomes and subgenomic mRNAs, the existence of other coronavirus RNA species, which are collectively referred to as noncanonical transcripts, has been suggested; however, their biological ...
Ching-Hung Lin +10 more
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RNA Isolation and Northern Blot Analysis
Bio-Protocol, 2014 The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao +5 more
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Frontiers in Microbiology, 2017 In bacteria, small non-coding RNAs (sRNAs) are critical regulators of cellular adaptation to changes in metabolism, physiology, or the external environment.
Xie Fuli +7 more
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Methods for the Detection of D-Amino-Acid Oxidase
Biological Procedures Online, 1998 Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
Konno Ryuichi
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melRNA-seq for Expression Analysis of SINE RNAs and Other Medium-Length Non-Coding RNAs
Mobile DNA, 2021 Background Small interspersed elements (SINEs) are transcribed by RNA polymerase III (Pol III) to produce RNAs typically 100–500 nucleotides in length.
Yoshinobu Mori, Kenji Ichiyanagi
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Atorvastatin action involves diminished recovery of hepatic HMG-CoA reductase activity
Journal of Lipid Research, 1998 The effects of atorvastatin on the expression of the hepatic HMG-CoA reductase and LDL receptor genes were investigated in rats. Like the other statins, atorvastatin increased the rate of degradation and presumably cycling of the hepatic LDL receptor. In
Gene C. Ness +2 more
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