Results 1 to 10 of about 57,892 (279)
Methods Enzymol, 2013 To measure the size and amount of RNA transcribed from a specific gene of interest.
He SL, Green R.
exaly +10 more sources
Protocol to detect m22G modification on single tRNA iso-decoders by immuno-northern blotting [PDF]
STAR ProtocolsSummary: tRNA modifications are highly dynamic and play crucial roles in regulating gene expression. Here, we present an assay for analyzing N2, N2-dimethylguanosine (m22G) modifications on tRNAs using an antibody-based detection approach.
Sseu-Pei Hwang +2 more
doaj +4 more sources
Immuno-Northern Blotting: Detection of RNA Modifications by Using Antibodies against Modified Nucleosides. [PDF]
PLoS ONE, 2015 The biological roles of RNA modifications are still largely not understood. Thus, developing a method for detecting RNA modifications is important for further clarification.
Eikan Mishima +13 more
doaj +2 more sources
Northern Blotting of RNA Denatured in Glyoxal Without Buffer Recirculation [PDF]
BioTechniques, 1997 A rapid and easy procedure for preparing Northern blots is described. RNA is denatured with glyoxal in the presence of ethidium bromide and glycerol, then electrophoresed through agarose in a buffer that does not require recirculation.
William V. Burnett
doaj +2 more sources
Protocol for rapid tRNA enrichment and chemiluminescent northern blot detection of tRNA and tRNA-derived fragments [PDF]
STAR ProtocolsSummary: Transfer RNA (tRNA), its post-transcriptional modifications, and tRNA-derived fragments (tRFs) play essential roles in cellular processes and gene regulation.
Pavlina Gregorova +3 more
doaj +2 more sources
Detection and Verification of Mammalian Mirtrons by Northern Blotting. [PDF]
Methods Mol Biol, 2018 microRNAs (miRNAs) have vital roles in regulating gene expression—contributing to major diseases like cancer and heart disease. Over the last decade, thousands of miRNAs have been discovered through high throughput sequencing-based annotation.
Zia MF, Flynt AS.
europepmc +3 more sources
Northern blotting analysis of microRNAs, their precursors and RNA interference triggers. [PDF]
BMC Mol Biol, 2011 Background Numerous microRNAs (miRNAs) have heterogeneous ends resulting from imprecise cleavages by processing nucleases and from various non-templated nucleotide additions.
Koscianska E +5 more
europepmc +3 more sources
Validation and characterization of murine gammaherpesvirus 68 antisense transcripts by northern blot analysis and quantitative reverse transcription-PCR [PDF]
Archives of Biological Sciences, 2023 The transcription of mammalian genomes exhibits an intriguing complexity and numerous novel RNA molecules have been identified. Viruses with large DNA genomes, especially herpesviruses, generate many different RNA species, including long non-coding RNAs (
Kara Mehmet
doaj +1 more source
Current Issues in Molecular Biology, 2021 Northern blotting (NB), a gold standard for RNA detection, has lost its charm due to its hands-on nature, need for good quality RNA, and radioactivity.
Waqar Ahmad +3 more
doaj +1 more source
Agronomy, 2021 RNA Interference (RNAi), which works against invading nucleic acids or modulates the expression of endogenous genes, is a natural eukaryotic regulating system, and it works by noncoding smaller RNA molecules.
Mohammad Faisal +2 more
doaj +1 more source