Methods for the Detection of D-Amino-Acid Oxidase
Biological Procedures Online, 1998 Four methods (an enzyme activity assay, western blotting, RT-PCR, and northern hybridization) to detect the enzyme D-amino-acid oxidase are described.
Konno Ryuichi
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Northern blotting confirmation of sRNA candidates in M. smegmatis. [PDF]
, 2013 Selected images of Northern blotting analysis for validated M. smegmatis sRNAs; the remaining images are included in Figure S1. Lane 1 and 2 indicate total RNA samples extracted from M. smegmatis and M. bovis BCG, respectively.
Lydia M. Contreras (484980) +5 more
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Atorvastatin action involves diminished recovery of hepatic HMG-CoA reductase activity
Journal of Lipid Research, 1998 The effects of atorvastatin on the expression of the hepatic HMG-CoA reductase and LDL receptor genes were investigated in rats. Like the other statins, atorvastatin increased the rate of degradation and presumably cycling of the hepatic LDL receptor. In
Gene C. Ness +2 more
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Evaluation of the Stress Effect on Expression of BEST-5 Gene in Gultured Rat Hepatocytes and Cloning of This Gene [PDF]
Journal of Kerman University of Medical Sciences, 2006 Introduction:Liver has important roles in body metabolic regulation and for this reason hepatocytes are used worldwide.Investigations showed that isolation of hepatocytes causes activation of stress related genes.The aim of this study was to study the ...
GH.H Hasanshahi +7 more
doaj
BMC Medical Genetics, 2006 Background Down syndrome (DS) is caused by trisomy 21 (+21), but the aberrations in gene expression resulting from this chromosomal aneuploidy are not yet completely understood.
Zigman Warren B +9 more
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Optimized RNA Extraction and Northern Hybridization in Streptomycetes
Biological Procedures Online, 2010 Northern blot hybridization is a useful tool for analyzing transcript patterns. To get a picture of what really occurs in vivo, it is necessary to use a protocol allowing full protection of the RNA integrity and recovery and unbiased transfer of the ...
Tagliavia Marcello +4 more
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Identification and characteristics of microRNAs from
BMC Genomics, 2008 Background MicroRNAs (miRNAs) are small RNA molecules that regulate gene expression by targeting messenger RNAs (mRNAs) and causing mRNA cleavage or translation blockage. Of the 355 Arthropod miRNAs that have been identified, only 21 are B.
Gao Xiaolian +11 more
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The gene encoding vitamin K-dependent anticoagulant protein S is expressed in multiple rabbit organs as demonstrated by northern blotting, in situ hybridization, and immunohistochemistry. [PDF]
, 1995 Vitamin K-dependent protein S is an anticoagulant plasma protein that functions as a co-factor to activated protein C in the degradation of coagulation factors Va and VIIIa.
A Bjartell, L Shen, B Dahlbäck, X He
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Bio-Protocol, 2013 Polysome profiling is a method that allows monitoring of translation activity of mRNAs in cells and tissues. Once each polysome fractions are collected, the translation activity of each mRNA is analyzed using various molecular biology techniques such as ...
Masahiro Morita +3 more
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Accumulation and processing patterns of chloroplast and mitochondrial tRNAs in PRORP1 RNAi mutants and wild-type plants as determined by northern blotting. [PDF]
, 2015 Detectable precursor RNA species are marked with asterisks. (A) Northern blot analysis of chloroplast tRNAs. Weaker exposures of the northern blots for tRNA-Glu(UUC), tRNA-Gln(UUG) and tRNA-Asp(GUC) are presented in S4 Fig.
Dirk Walther (32589) +5 more
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