Results 21 to 30 of about 57,892 (279)

Primers used for gene cloning and northern blotting. [PDF]

open access: yes, 2016
Primers used for gene cloning and northern blotting.
Toshihiro Suzuki (278774)   +4 more
core   +1 more source

Blotting techniques [PDF]

open access: yes, 2005
The capacity to separate nucleic acids and proteins by their molecular weight and identify fractions of interest by the hybridization of specific labeled probes is known as blotting.
Hibbitts, Samantha Jayne, White, P. L.
core   +1 more source

Figure 1D - tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by Northern blotting analysis [PDF]

open access: yes, 2018
tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by northern blotting analysis. Total tRNA was extracted from cells growing in MOPS minimal medium with 0.2% glucose as the carbon source before and after isoleucine ...
Gerdes, K
core   +3 more sources

Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo

open access: yesVirology Journal, 2023
Background In addition to the well-known coronavirus genomes and subgenomic mRNAs, the existence of other coronavirus RNA species, which are collectively referred to as noncanonical transcripts, has been suggested; however, their biological ...
Ching-Hung Lin   +10 more
doaj   +1 more source

Highly Sensitive Northern Hybridization Using a Rapid Protocol for Downward Alkaline Blotting of RNA

open access: yesBioTechniques, 1998
A simple and fast RNA gel blot procedureis described that uses 50 mM NaOH tosimultaneously transfer and fix RNA to apositively charged nylon membrane. TheRNA is transferred in a downward direction,and transfer is routinely completedwithin 2.5 h.
Ivan L. Ingelbrecht   +2 more
doaj   +1 more source

Immuno-northern blotting using antibodies against modified nucleosides. [PDF]

open access: yes, 2015
(A) Structural formulas of 1-methyladenosine (m1A), N6-methyladenosine (m6A), pseudouridine (Ψ), and 5-methylcytidine (m5C). (B) Total RNA (0.6 μg) isolated from the indicated samples was separated in the 12% polyacrylamide-8 M urea denaturing gel and ...
Kuniyasu Niizuma (830646)   +13 more
core   +1 more source

RNA Isolation and Northern Blot Analysis

open access: yesBio-Protocol, 2014
The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA in a sample. With northern blotting it is possible to observe particular gene expression levels during differentiation, morphogenesis, as ...
Ying Liao   +5 more
doaj   +1 more source

melRNA-seq for Expression Analysis of SINE RNAs and Other Medium-Length Non-Coding RNAs

open access: yesMobile DNA, 2021
Background Small interspersed elements (SINEs) are transcribed by RNA polymerase III (Pol III) to produce RNAs typically 100–500 nucleotides in length.
Yoshinobu Mori, Kenji Ichiyanagi
doaj   +1 more source

A Genome-Wide Prediction and Identification of Intergenic Small RNAs by Comparative Analysis in Mesorhizobium huakuii 7653R

open access: yesFrontiers in Microbiology, 2017
In bacteria, small non-coding RNAs (sRNAs) are critical regulators of cellular adaptation to changes in metabolism, physiology, or the external environment.
Xie Fuli   +7 more
doaj   +1 more source

Northern blotting confirmation of sRNA candidates in M. bovis BCG. [PDF]

open access: yes, 2013
Selected images of Northern blotting analysis for validated M. bovis BCG sRNAs; the remaining images are included in Figure S2. Lane 1 and 2 indicate total RNA samples extracted from M. bovis BCG and M. smegmatis, respectively.
Lydia M. Contreras (484980)   +5 more
core   +1 more source

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