Results 11 to 20 of about 57,892 (279)
Total Bacterial RNA Isolation and Northern Blotting Analysis [PDF]
, 2023 The study of bacterial gene expression during infection provides vital information for researchers to understand bacterial pathogenesis and infection. The ability to obtain clean and undegraded RNA could be challenging and daunting and remains the most ...
Loh, E,, Karlsson, J,, Eichner, H,
core +1 more source
HDAC11-S4 RNA 4 was validated by Northern blotting and in situ hybridization. [PDF]
, 2023 A, Validation of HDAC11-S4 RNA 4 by Northern blotting. The total RNA extracted from the BmCPV-infected midgut was separated on 1% agarose-formaldehyde gels (left) and transferred to Hybond-N+ nylon membranes.
Chengliang Gong (299403) +7 more
core +1 more source
Rapid amplification of cDNA ends (RACE) and northern blotting of the 5′ UTR of the CO1 mRNA. [PDF]
, 2021 (A) RACE of the 5′ UTR of CO1 mRNA in WT (control) and FASTKDs-KO HAP1 cells. (B) Sanger sequencing of the 5′ UTR of CO1 mRNA from WT HAP1 cells. (C) RACE of the 5′ end of CO2 mRNA in WT (control) and FASTKDs-KO HAP1 cells.
Akira Ohkubo (2955393) +8 more
core +1 more source
Isolation and detection of small RNA molecules
Plant, Soil and Environment, 2007 The article describes in detail methodical procedures for isolation of small RNA molecules, their electrophoresis, northern blotting and finally detection by hybridization with radioactively labeled riboprobes.
J. Fulneček
doaj +1 more source
Primary culture and mRNA analysis of human ovarian cells
Biological Procedures Online, 2002 Established cell lines are invaluable for studying cell and molecular biological questions. A variety of human ovarian cancer (OC) cell lines exist, however, most have acquired significant genetic alterations from their cells of origin, including ...
Dunfield Lesley D. +2 more
doaj +1 more source
Polyclonal antibodies against potato spindle tuber viroid RNA
Frontiers in Bioscience-Elite, 2022 Ribonucleic acid (RNA) can act as a hapten in the direct immunization of animals. For antigen synthesis, 65 mg of viroid RNA were obtained by in vitro transcription of the recombinant DNA. We received a reasonable immune response in mice and rabbits with
Yuri Drygin +2 more
doaj +1 more source
Proceedings of the Nutrition Society, 1996 Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by Northern blotting. A cDNA radioactively labelled with 32P is the most-commonly-used hybridization probe.
openaire +2 more sources
Oligos for northern blotting (dsDNA probes). [PDF]
, 2013 Oligos for northern blotting (dsDNA probes).
Sara C. Cloutier (487136) +4 more
core +1 more source
Northen blotting with DIG Northern Starter Kit (Roche®) v1 [PDF]
, 2016 This script is used for northern blotting with the Roche DIG Northern Starter Kit.
Lutz Berwanger
core +1 more source