Results 11 to 20 of about 178,608 (339)

Near-infrared fluorescent northern blot. [PDF]

open access: yesRNA, 2018
Northern blot analysis detects RNA molecules immobilized on nylon membranes through hybridization with radioactive 32P-labeled DNA or RNA oligonucleotide probes. Alternatively, nonradioactive northern blot relies on chemiluminescent reactions triggered by horseradish peroxidase (HRP) conjugated probes.
Miller BR   +4 more
europepmc   +4 more sources

Northern blotting [PDF]

open access: bronzeProceedings of the Nutrition Society, 1996
Northern blotting is the main method for examining the expression of genes through measurement of an mRNA. Tissue specificity and the factors which regulate expression can both be determined by Northern blotting. A cDNA radioactively labelled with 32P is the most-commonly-used hybridization probe.
Paul Trayhurn
openaire   +3 more sources

Northern Blot Protocol v1 [PDF]

open access: gold, 2020
Northern blot is a hybridization technique to visualize and quantify RNAs from cells or tissues using a radio-labeled probe. mRNAs only constitute a small fraction of total RNA in cells. Through enrichment of poly(A)-tailed mRNAs, this protocol provides increased sensitivity for the detection of lowly expressed mRNAs.
Sibylle Mitschka, Christine Mayr
openaire   +2 more sources

A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants [PDF]

open access: yes, 2013
Translation is an important step in gene expression. The initiation of translation is phylogenetically diverse, since currently five different initiation mechanisms are known.
Gäbel, Katrin   +4 more
core   +9 more sources

A New Biotin Labeling and High-Molecular-Weight RNA Northern Method and Its Application in Viral RNA Detection

open access: yesViruses, 2022
Viruses cause severe crop losses. Studying the interaction between viruses and plants is very important for development of control measures. Northern blot is a well-accepted but very challenging technique to monitor the infection of viruses.
Xi Zhang   +8 more
doaj   +1 more source

Overexpression of a stilbene synthase gene from Fallopia multiflora causes trans-piceid accumulation in transgenic Arabidopsis

open access: yesBiologia Plantarum, 2020
Fallopia multiflora, also known as Polygonum multiflorum, is a traditional Chinese medicine with stilbenes as the main characteristic components. We previously isolated the presumed stilbene synthase gene FmSTS1 from F. multiflora and inserted the FmSTS1
S. SHENG   +7 more
doaj   +1 more source

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