Results 291 to 300 of about 178,608 (339)
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Current Protocols in Toxicology, 2001
AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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Detection of MicroRNAs by Northern Blot
2023Small RNAs (sRNAs) are key regulators of transcriptomes and proteomes of organisms through their sequence-specific interaction with complementary RNA targets. sRNAs can be classified according to their origin and mode of action into different classes such as: microRNAs (miRNAs), small interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs).
Martinho, Claudia, Lopez-Gomollon, Sara
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Nonradioactive Northern Blot of circRNAs
2018Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient ...
Xiaolin, Wang, Ge, Shan
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Analysis of Gene Expression: Northern Blotting
2003Messenger (m)RNAs represent the information-carrying intermediates for protein synthesis. Northern blotting gives a snapshot of the relative abundance of an RNA transcript at a set time point by measuring the steady-state levels of an RNA transcript.
J, Rousell, el-B, Haddad
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Detecting sRNAs by Northern Blotting
2011Small RNAs (sRNAs) play fundamental roles in modifying the transcriptomes and proteomes of organisms. sRNAs can be classified according to their origin and way of action into classes such as microRNAs, trans-acting siRNAs, heterochromatin siRNAs, Piwi-interacting RNAs, and natural antisense siRNAs, although microRNAs are the most conserved and studied ...
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Northern Blotting Techniques for Small RNAs
2010In eukaryotes, RNA silencing encompasses a range of biochemical processes mediated by approximately 20-25 nt small RNAs (smRNAs). This chapter describes northern blot hybridization techniques optimized for detection of such smRNAs, whether extracted from plant or animal tissues.
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A systematic approach to quantitative Western blot analysis
Analytical Biochemistry, 2020Lakshmi Pillai-Kastoori
exaly

