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Northern Blot Analysis of RNA

Current Protocols in Toxicology, 2001
AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
openaire   +2 more sources

Detection of MicroRNAs by Northern Blot

2023
Small RNAs (sRNAs) are key regulators of transcriptomes and proteomes of organisms through their sequence-specific interaction with complementary RNA targets. sRNAs can be classified according to their origin and mode of action into different classes such as: microRNAs (miRNAs), small interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs).
Martinho, Claudia, Lopez-Gomollon, Sara
openaire   +3 more sources

Nonradioactive Northern Blot of circRNAs

2018
Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient ...
Xiaolin, Wang, Ge, Shan
openaire   +2 more sources

Analysis of Gene Expression: Northern Blotting

2003
Messenger (m)RNAs represent the information-carrying intermediates for protein synthesis. Northern blotting gives a snapshot of the relative abundance of an RNA transcript at a set time point by measuring the steady-state levels of an RNA transcript.
J, Rousell, el-B, Haddad
openaire   +2 more sources

Detecting sRNAs by Northern Blotting

2011
Small RNAs (sRNAs) play fundamental roles in modifying the transcriptomes and proteomes of organisms. sRNAs can be classified according to their origin and way of action into classes such as microRNAs, trans-acting siRNAs, heterochromatin siRNAs, Piwi-interacting RNAs, and natural antisense siRNAs, although microRNAs are the most conserved and studied ...
openaire   +2 more sources

Northern blotting

1998
Jeanne Dijkstra, Cees P. de Jager
  +4 more sources

Northern Blotting Techniques for Small RNAs

2010
In eukaryotes, RNA silencing encompasses a range of biochemical processes mediated by approximately 20-25 nt small RNAs (smRNAs). This chapter describes northern blot hybridization techniques optimized for detection of such smRNAs, whether extracted from plant or animal tissues.
openaire   +4 more sources

Quantitative paper-based dot blot assay for spike protein detection using fuchsine dye-loaded polymersomes

Biosensors and Bioelectronics, 2021
Faezeh Ghorbani-zamani   +2 more
exaly  

A systematic approach to quantitative Western blot analysis

Analytical Biochemistry, 2020
Lakshmi Pillai-Kastoori
exaly  

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