Results 91 to 100 of about 54,464 (302)

Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA, miRNA and piRNA by northern blot [PDF]

, 2007
Gurman S. Pall, Carles Codony, John Byrne, L. Ritchie, Andrew J. Hamilton   +4 more
openalex   +1 more source

Assessing the viability of genebanked seeds from rare, wild plants native to the United States using the D.E.A.D. paradigm

Applications in Plant Sciences, EarlyView.
Abstract Premise Genebanks must maintain viable seeds for decades. Seeds that germinate are clearly alive, but some seeds, often from wild populations, do not germinate because they are dormant, empty, aged, or damaged (D.E.A.D.). This work evaluates the effects of D.E.A.D.
Christina Walters, Lisa M. Hill, Katherine D. Heineman, Hannah M. Tetreault, Shaimaa Ibrahim, Katherine Markstein, Cheryl Birker, Kris Freitag, Dustin Wolkis, Michael Kunz, Sheila Murray, Nathaniel Kingsley, Alexandra Seglias, Nicholas Matsumoto, Thomas N. Kaye, Cheryl Peterson, Matthew A. Albrecht, Noah Dell, Stacy Anderson, Steve Blackwell, David Remucal, Wendy Gibble, Anita Tiller, Emily Coffey, Jason Ligon, Laurie Blackmore, Carrie Radcliffe, Heather E. Schneider, Kristen Nelson, David Sollenberger, Jessamine Finch, Kate Wellspring, Robert Jetton, Joyce Maschinski   +33 more
wiley   +1 more source

Establishment of Interspecies Somatic Cell Nuclear Transfer and Transgene‐Free Inducible Pluripotent Stem Cells for Versatile Conservation of the Germplasm Resource of Wild Boar

Animal Research and One Health, EarlyView.
Conserving genetic material and even increasing genetic diversity is critical. To conduct the conservation of wild boar germplasm resources, we have successfully obtained healthy cloned wild boars for the first time using interspecies somatic cell nuclear transfer and established transgene‐free iPSCs that can be used to conduct iterative rounds of gene
Chen Gao, Xinyi Zhou, Shigang Gu, Jianbo Li, Jing Wang, Yurong Zhang, Wenxuan Zhao, Xinhua Wei, Liting Lu, Quanmin Zhao, Yongye Huang, Dawei Yu   +11 more
wiley   +1 more source

Detection of mRNA by in situ hybridisation and in northern blot analysis using oligodeoxynucleotide probes labelled with alkaline phosphatase. [PDF]

, 1992
M. A. Farquharson, Rozelle Harvie, Ann C. Kennedy, Anne Couvelard   +3 more
openalex   +1 more source

Diagnostic Implications and Correlates of Plasma Adenosine Deaminase 2 Activity and ADA2 Variants

Arthritis &Rheumatology, EarlyView.
Objective Deficiency of adenosine deaminase 2 (DADA2) is a monogenic autoinflammatory disease manifested as polyarteritis nodosa, stroke, and bone marrow failure. Leveraging an international cohort of 200 DADA2 cases, we aimed to characterize the diagnostic utility of a plasma ADA2 enzyme activity assay and understand the implications of residual ADA2 ...
Jian Yue, Courtney E. LeSon, Casey A. Rimland, Kailey Brodeur, Rachel Weng, Evan E. Hsu, Michael T. Lam, Manpreet Meyer, Seigo Okada, Andrew L. D. Hsu, Musaab A. Alhezam, Megan M. Perron, Olha Halyabar, Fatma Dedeoglu, Peter A. Nigrovic, Akiko Shimamura, R. Grant Rowe, Megan A. Cooper, Tiphanie P. Vogel, Miriah Gillispie‐Taylor, Rosemary Peterson, Clara Lin, Katherine D. Nowicki, Kaylee Dollerschell, Taizo A. Nakano, Roxane Labrosse, Alexis‐Virgil Cochino, Andreea Ioan, Leonardo Oliveira Mendonca, Samar Freschi Barros, Jorge Kalil, Javier Perez‐Rojas, Alicia Diaz‐Kuan, Eric S. Sobel, Taha Moussa, Samuel Shang Ming Lee, Li Wearn Koh, Archana Khan, Sodality Sutnga, Raju Khubchandani, Walaa Shoman, Yasmine El Chazli, Benzeeta Pinto, Chengappa Kavadichanda, Roshini S. Abraham, Xiangwei Sun, Qing Zhou, Gsrsnk Naidu, Aman Sharma, Carl Esperanzate, Daniel L. Kastner, Ivona Aksentijevich, Susan J. Kelly, Michael Hershfield, Teresa K. Tarrant, Pui Y. Lee   +55 more
wiley   +1 more source

Immediate visualization of blotted RNA in Northern analysis [PDF]

, 1989
Richard A. Kroczek
openalex   +1 more source

Intracellular self‐assembled nanoneedles for efficient lysosomal escape and CRISPR/Cas9 mediated gene editing

BMEMat, EarlyView.
PlaMnB was formed by cytolysin A‐tumor homing peptides (THP)‐modified bacterial membranes encapsulating the clustered regularly interspaced short palindromic repeat/CRISPR‐associated protein (CRISPR/Cas9) plasmid and manganese dioxide. THP‐containing bacterial membrane enhances PlaMnB vascular permeability and tumor targeting, and after cellular ...
Chen Zhang, Nan Zhang, Ruiqi Yang, Qian Qiu, Jiaying Huang, Jingang Liu, Silu Li, Jonathan F. Lovell, He Ren, Yumiao Zhang   +9 more
wiley   +1 more source

Mapping molluscan endocrinology: a systematic and critical appraisal

Biological Reviews, EarlyView.
ABSTRACT Historically, a vertebrate‐centric paradigm has framed our interpretation of molluscan endocrinology, with considerable research focusing on vertebrate‐type steroid hormones (e.g. oestrogens, testosterone). However, contradictory evidence on the occurrence of vertebrate‐type steroid hormones in molluscan tissues, and a lack of the specific ...
Konstantinos Panagiotidis, Thomas H. Miller, Olwenn V. Martin, Alice Baynes   +3 more
wiley   +1 more source

Infection Risk From Humans and Animals in the Anatomy Laboratory: A Scoping Review

Clinical Anatomy, EarlyView.
ABSTRACT Whole‐body dissection is a cornerstone of anatomy education. During and following the COVID‐19 pandemic, exposure to infectious agents and other risks of dissection were highlighted. To identify potential risks, one must have the data outlining these risks in specific situations.
Margaret A. McNulty, Elizabeth R. Agosto
wiley   +1 more source

Recombinant Monoclonal Antibodies for Detecting the Tubulin Post‐Translational Modifications Glutamylation and Lysine‐40 Acetylation

Cytoskeleton, EarlyView.
ABSTRACT Post‐translational modifications (PTMs) to tubulin subunits in microtubule filaments are thought to comprise a component of the tubulin code that specifies microtubule functions in cell physiology and animal development. Acetylation of Lysine‐40 (K40) on α‐tubulin (αTub‐K40ac) and glutamylation of both α‐ and β‐tubulin are two tubulin PTMs of ...
Lynne Blasius, Ezekiel C. Thomas, Erik H. Collet, Erin Jenson, Chad G. Pearson, Ryoma Ohi, Kristen J. Verhey   +6 more
wiley   +1 more source