Results 81 to 90 of about 57,892 (279)

Isolation of Polysome-bound mRNA from Rice Solid Tissues Amenable for RT-PCR and Profiling Experiments

Bio-Protocol, 2015
Polysome profile analysis is a frequently performed task in translational control research that not only enables direct monitoring of the efficiency of translation but can easily be extended with a wide range of downstream applications such as Northern ...
Mehdi Jabnoune, David Secco, Cécile Lecampion, Christophe Robaglia, Qingyao Shu, Yves Poirier   +5 more
doaj   +1 more source

Northern blotting. [PDF]

, 2012
(A) The blot was hybridized with Dig-labeled BjATl RNA probe. The arrow indicates the position of molecular size equivalent to 2000 bp. (B) A total of 5 µg RNA was analyzed in 1.2% agarose formaldehyde-denaturing gel.
Yeqing Chao (177970), Yujun Liang (177976), Bei Gao (59773), Chunxin Fan (177973), Shicui Zhang (38479)   +4 more
core   +1 more source

A Pancreatitis‐Inspired Trypsinogen Nanoplatform Reprograms Tumor‐Associated Macrophages via NF‐κB for Pancreatic Cancer Immunotherapy

Advanced Science, EarlyView.
Trypsinogen‐loaded nanoparticles reprogram TAMs via NF‐κB/NLRP3, driving M2→M1 conversion and potent phagocytosis to unleash antitumor immunity. ABSTRACT Although reprogramming tumor‐associated macrophages (TAMs) represents a promising therapeutic strategy, approaches that are both precise and safe remain scarce.
Lei Cao, Yu He, Wenhao Li, Bo Gong, Han Lin, Jianlin Shi, Yuan Yong, Wencheng Wu   +7 more
wiley   +1 more source

Rapid and efficient isolation of high-quality small RNAs from recalcitrant plant species rich in polyphenols and polysaccharides.

PLoS ONE, 2014
Small RNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), are important regulators of plant development and gene expression. The acquisition of high-quality small RNAs is the first step in the study of its expression and function ...
Jun Peng, Zihao Xia, Ling Chen, Minjing Shi, Jinji Pu, Jianrong Guo, Zaifeng Fan   +6 more
doaj   +1 more source

Porcine Deltacoronavirus M Protein Binds NLRP3 to Promote Inflammasome Assembly via Competition with TRIM31

Advanced Science, EarlyView.
Porcine deltacoronavirus (PDCoV) infection induces severe intestinal inflammation and acute diarrhea in piglets, yet the molecular mechanism remains incompletely understood. The M protein activates NLRP3 inflammasome through dual mechanisms: direct binding to the NLRP3 LRR domain and disruption of TRIM31‐mediated K48‐linked ubiquitination.
Jinhui Hou, Fangfang Han, Anqi Liu, Yang Yu, Rui Zhao, Zhengting Shi, Yanrong Lv, Jinli Liu, Shaopo Zu, Zhanyong Wei, Jin Yuan, Hui Hu   +11 more
wiley   +1 more source

Northern blotting of the (Ago)shRNA processing products. [PDF]

, 2015
Processing of the 5’ strand (upper panels) and 3’ strand (lower panels) of AgoshGag5 and AgoshPol1 variants was analyzed by Northern blot analysis. The asterisks indicate the regular Dicer processed small RNAs of 21 nt and the Ago2 products of ~33 nt ...
Elena Herrera-Carrillo (757203), Ben Berkhout (7015), Ying Poi Liu (757201), Margarete Karg (757202)   +3 more
core   +1 more source

Psychological Stress Associated Bile Acid Reprogramming Promotes Hepatocellular Carcinoma Progression

Advanced Science, EarlyView.
Depression is increasingly recognized as a risk factor for chronic diseases, yet its biological impact on cancer remains unclear. Using data from more than 490 000 participants across three international cohorts, we show that depression significantly increases the risk of liver cancer.
Ruijiang Zeng, Mengmeng Wang, Kang Wang, Zhuo Xing, Yulong Hong, Tongtong Li, Geng Zong, Chong Yang, Dan Zhang, Zhangling Chen, Xin Jin   +10 more
wiley   +1 more source

Efeito do estresse térmico agudo sobre os níveis da proteína e RNA mensageiro da Hsp70, em fígado e cérebro de pintos de corte de diferentes linhagens

Revista Brasileira de Zootecnia, 2001
Oitenta pintos de corte de duas linhagens, pescoço pelado (Na/na) e Hubbard-Pettersen, entre 2 e 5 dias de idade, foram expostos a estresse térmico agudo (36-37°C), durante cinco horas.
Nelson José Laurino Dionello, Jesus Aparecido Ferro, Marcos Macari, Fernando Rutz, Luiz Roberto Furlan, Maria Inês Tiraboschi Ferro   +5 more
doaj  

Detection of novel and differentially expressed miRNAs by northern blotting. [PDF]

, 2014
A) Detection of novel miRNAs RNA; B) Detection of differentially expressed miRNAs. Northern blots of total RNA isolated from leaves of mock (M) and infected (I) leaves were probed with labeled oligonucleotides. The U6 RNA was used as internal control.
Fangli Wu (516340), Weibo Jin (14025), Jianhong Shu (516341)   +2 more
core   +1 more source

Kaempferol Attenuates Spaceflight‐Associated Knee Cartilage Degradation by Targeting NOX4‐Mediated Mitochondrial Dysfunction

Advanced Science, EarlyView.
We have shown that both simulated and actual spaceflight contribute to cartilage degradation in the knees of mice. Using RNA sequencing and bioenergetic profiling, we identified NADPH oxidase 4 (NOX4) as a key factor driving these changes. Additionally, we found that kaempferol, a naturally occurring flavonoid that binds directly to NOX4, reduces ...
Yuesong Yin, Ruiling Xu, Meagan J. Makarczyk, Jia‐Jun Liu, Silvia Liu, Matthew Z. Shi, Roberta Di Carlo, Michael F. Almeida, Alex Garris, Sarah Day, Rebekah Sanchez‐Hodge, Jonathan C. Schisler, Aleeza H. Zilberman, Noah G. Allen, Angela J. Kubik, Elizabeth A. Blaber, Arnold Z. Olali, Wanqing Xie, Douglas C. Wallace, Christopher E. Mason, Peter G. Alexander, Giuseppe Intini, Afshin Beheshti, Hang Lin   +23 more
wiley   +1 more source