Results 251 to 260 of about 54,464 (302)
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2003
Northern blotting is the technique whereby RNA molecules are denatured, fractionated through agarose on the basis of size, and then transferred to a solid matrix for subsequent hybridization to a specific labeled probe. The technique can be used to analyze the pattern of transgene expression in an organism, and changes in that pattern following ...
J W, Pollard, C R, Perry, C F, Thurston
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Northern blotting is the technique whereby RNA molecules are denatured, fractionated through agarose on the basis of size, and then transferred to a solid matrix for subsequent hybridization to a specific labeled probe. The technique can be used to analyze the pattern of transgene expression in an organism, and changes in that pattern following ...
J W, Pollard, C R, Perry, C F, Thurston
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2010
Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound.
Josefsen, Knud, Nielsen, Henrik
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Northern blotting analysis is a classical method for analysis of the size and steady-state level of a specific RNA in a complex sample. In short, the RNA is size-fractionated by gel electrophoresis and transferred by blotting onto a membrane to which the RNA is covalently bound.
Josefsen, Knud, Nielsen, Henrik
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Analysis of RNA by Northern Blotting
Cold Spring Harbor Protocols, 2022Northern hybridization is used to measure the amount and size of RNAs transcribed from eukaryotic genes and to estimate their abundance. No other method is capable of obtaining these pieces of information simultaneously from a large number of RNA preparations; northern analysis is therefore fundamental to studies of gene expression in eukaryotic cells.
Michael R, Green, Joseph, Sambrook
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BlotBase: A northern blot database
Gene, 2008With the availability of high-throughput gene expression analysis, multiple public expression databases emerged, mostly based on microarray expression data. Although these databases are of significant biomedical value, they do hold significant drawbacks, especially concerning the reliability of single gene expression profiles obtained by microarray ...
K, Schlamp +5 more
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Current Protocols in Toxicology, 2001
AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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Detection of MicroRNAs by Northern Blot
2023Small RNAs (sRNAs) are key regulators of transcriptomes and proteomes of organisms through their sequence-specific interaction with complementary RNA targets. sRNAs can be classified according to their origin and mode of action into different classes such as: microRNAs (miRNAs), small interfering RNAs (siRNAs) and PIWI-interacting RNAs (piRNAs).
Martinho, Claudia, Lopez-Gomollon, Sara
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Nonradioactive Northern Blot of circRNAs
2018Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient ...
Xiaolin, Wang, Ge, Shan
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Analysis of Gene Expression: Northern Blotting
2003Messenger (m)RNAs represent the information-carrying intermediates for protein synthesis. Northern blotting gives a snapshot of the relative abundance of an RNA transcript at a set time point by measuring the steady-state levels of an RNA transcript.
J, Rousell, el-B, Haddad
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Detecting sRNAs by Northern Blotting
2011Small RNAs (sRNAs) play fundamental roles in modifying the transcriptomes and proteomes of organisms. sRNAs can be classified according to their origin and way of action into classes such as microRNAs, trans-acting siRNAs, heterochromatin siRNAs, Piwi-interacting RNAs, and natural antisense siRNAs, although microRNAs are the most conserved and studied ...
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