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BlotBase: A northern blot database
Gene, 2008With the availability of high-throughput gene expression analysis, multiple public expression databases emerged, mostly based on microarray expression data. Although these databases are of significant biomedical value, they do hold significant drawbacks, especially concerning the reliability of single gene expression profiles obtained by microarray ...
K, Schlamp +5 more
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, 2008 Current Protocols in Toxicology, 2001
AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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AbstractThis unit describes the processes of extracting RNA from tissues and analyzing it by northern blot hybridization to probe the expression of a particular gene at the transcriptional level.
M, Bergeron, J, Honkaniemi, F R, Sharp
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Nonradioactive Northern Blot of circRNAs
2018Circular RNAs (circRNAs) are recognized as a special species of transcripts in metazoans with increasing studies, and northern blotting is a direct way to confirm the existence and to evaluate the size of individual circRNAs. Northern blotting probes can be radioactive isotope (32P) labeled, which is not environment-friendly and sometimes inconvenient ...
Xiaolin, Wang, Ge, Shan
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Analysis of RNA by Northern Blot Hybridization
Current Protocols in Human Genetics, 2001AbstractSpecific sequences in RNA preparations can be detected by blotting and hybridization analysis using techniques very similar to those originally developed for DNA. Fractionated RNA is transferred from an agarose gel to a membrane support (northern blotting); unfractionated RNA is immobilized by slot or dot blotting.
T, Brown, K, Mackey
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Detecting sRNAs by Northern Blotting
2011Small RNAs (sRNAs) play fundamental roles in modifying the transcriptomes and proteomes of organisms. sRNAs can be classified according to their origin and way of action into classes such as microRNAs, trans-acting siRNAs, heterochromatin siRNAs, Piwi-interacting RNAs, and natural antisense siRNAs, although microRNAs are the most conserved and studied ...
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Analysis of RNA by Northern-Blot Hybridization
2003Northern-blot hybridization, also referred to as Northern blotting, is one of several methods developed to detect the presence, to determine the size, and to quantify specific cellular mRNAs. By this method, total RNA or poly(A)(+)mRNA, prepared from the cells or tissue of interest, is fractionated by size on a denaturing agarose gel.
P E, Gallagher, D I, Diz
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