The impact of PCR duplication on RNAseq data generated using NovaSeq 6000, NovaSeq X, AVITI, and G4 sequencers [PDF]
Genome Biology, 2023 Background Transcriptome sequencing (RNA-seq) is a powerful technology for gene expression profiling. Selection of optimal parameters for cDNA library generation is crucial for acquisition of high-quality data. In this study, we investigate the impact of
Natalia Zajac +2 more
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Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis [PDF]
BMC Genomics, 2021 Background GenoLab M is a recently established next-generation sequencing platform from GeneMind Biosciences. Presently, Illumina sequencers are the globally leading sequencing platform in the next-generation sequencing market. Here, we present the first
Zhiliang Zhou, Liu Yongfeng
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Comparative analysis of NovaSeq 6000 and MGISEQ 2000 single‐cell RNA sequencing data
Quantitative Biology, 2022 Background Single‐cell RNA sequencing (scRNA‐seq) technology is now becoming a widely applied method of transcriptome exploration that helps to reveal cell‐type composition as well as cell‐state heterogeneity for specific biological processes.
Yixue Li, Guangyong Zheng
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Correction to: Comparative performance of the GenoLab M and NovaSeq 6000 sequencing platforms for transcriptome and LncRNA analysis [PDF]
BMC Genomics, 2022 Yongfeng Liu +8 more
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Interdisciplinary MedicineSingle‐cell spatial transcriptomics enables comprehensive gene expression profiling with precise cellular localization within tissue architecture. To systematically evaluate the compatibility and performance of alternative sequencing platforms for this ...
Guangchuang Yu
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A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection [PDF]
Genome BiologyBackground The sequencing-by-synthesis technology by Illumina, Inc. enables efficient and scalable readouts of mutations from genomic data. To enhance sequencing speed and efficiency, Illumina has shifted from the four-color base calling chemistry of the
Beverly J. Fu +4 more
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A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge [PDF]
mSystemsDriven by the increasing scale of microbiome studies and the rise of large, continuously expanding population cohorts, the volume of sequencing data is growing rapidly.
Kinga Zielińska +4 more
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Surface sediments prokaryotic communities: five years of 16S rRNA amplicon sequencing data from the northernmost part of the Mediterranean SeaSRA - NCBI [PDF]
Data in BriefSurface sediments harbour diverse prokaryotic communities that play a key role in biogeochemical cycling and provide valuable insights when compared with water column communities, allowing for a more comprehensive understanding of marine ecosystem ...
Noemi Tomasi +3 more
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Comparative assessment of the Sikun 2000 sequencing platform for whole genome sequencing [PDF]
Scientific ReportsDNA sequencing technology has significantly advanced over the past five decades, particularly with the introduction of next-generation sequencing (NGS) platforms like Illumina’s NovaSeq.
Keya Cai +10 more
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The complete mitochondrial genome of Myotis chinensis (Chiroptera: Vespertilionidae) and its phylogenetic analysis [PDF]
Mitochondrial DNA. Part B. ResourcesThe vesper bat, Myotis chinensis, is a member of the family Vespertilionidae and is found in Southeast Asia. There are, to date, no reports of the complete mitochondrial genome of this species.
Xinyu Miao, Lizhi Zhou
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