Results 1 to 10 of about 173,111 (241)

The impact of PCR duplication on RNAseq data generated using NovaSeq 6000, NovaSeq X, AVITI, and G4 sequencers

Genome Biology
Background Transcriptome sequencing (RNA-seq) is a powerful technology for gene expression profiling. Selection of optimal parameters for cDNA library generation is crucial for acquisition of high-quality data. In this study, we investigate the impact of
Natalia Zajac
exaly   +3 more sources

Comparison of Illumina NovaSeq 6000, GeneMind SURFSeq 5000, Salus Evo, and MGI DNBSEQ-G400 for Ancient DNA Whole-Genome Sequencing. [PDF]

Genes
Background: Ancient DNA (aDNA) research is one of the biological fields that has been transformed by the development of next-generation sequencing (NGS). To date, Illumina sequencing has dominated aDNA research.
Natalia Svirkina, Tatiana Andreeva, Andrey Manakhov   +2 more
exaly   +3 more sources

Chromosome-level haplotype-resolved genome assembly for Takifugu ocellatus using PacBio and Hi-C technologies

Scientific Data, 2023
Measurement(s) Whole Genome Sequencing • Chromosome assembly by Hi-C data • Whole Transcriptome Sequencing Technology Type(s) PacBio Sequel System • Hi-C • Illumina HiSeq. 2500 • Illumina NovaSeq.
Qingmin Zeng, Zhixiong Zhou, Qian He, Leibin Li, Fei Pu, Mengzhen Yan, Peng Xu   +6 more
doaj   +2 more sources

Chromosomal-level genome assembly of potato tuberworm, Phthorimaea operculella: a pest of solanaceous crops

Scientific Data, 2022
Measurement(s) whole genome sequencing Technology Type(s) Illumina NovaSeq. 6000 • Oxford Nanopore Sequencing Sample Characteristic - Organism Phthorimaea operculella Sample Characteristic - Location Yunnan Province, Southwest ...
Mengdi Zhang, Xinyue Cheng, Runmao Lin, Bingyan Xie, Ralf Nauen, Silvia I. Rondon, Jorge A. Zavala, Subba Reddy Palli, Suhua Li, Xingyao Xiong, Wenwu Zhou, Yulin Gao   +11 more
doaj   +2 more sources

A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection [PDF]

Genome Biology
Background The sequencing-by-synthesis technology by Illumina, Inc. enables efficient and scalable readouts of mutations from genomic data. To enhance sequencing speed and efficiency, Illumina has shifted from the four-color base calling chemistry of the
Beverly J. Fu, Vinayak V. Viswanadham, Dominika Maziec, Hu Jin, Peter J. Park   +4 more
doaj   +2 more sources

Surface sediments prokaryotic communities: five years of 16S rRNA amplicon sequencing data from the northernmost part of the Mediterranean SeaSRA - NCBI [PDF]

Data in Brief
Surface sediments harbour diverse prokaryotic communities that play a key role in biogeochemical cycling and provide valuable insights when compared with water column communities, allowing for a more comprehensive understanding of marine ecosystem ...
Noemi Tomasi, Elisa Banchi, Vincenzo Manna, Mauro Celussi   +3 more
doaj   +2 more sources

A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge [PDF]

mSystems
Driven by the increasing scale of microbiome studies and the rise of large, continuously expanding population cohorts, the volume of sequencing data is growing rapidly.
Kinga Zielińska, Kateryna Pantiukh, Pawel P. Łabaj, Tomasz Kosciolek, Elin Org   +4 more
doaj   +2 more sources

Comparison of Illumina NovaSeq 6000 and GeneMind SURFSeq 5000 platforms for single-cell spatial transcriptomics of mouse brain and lung

Interdisciplinary Medicine
Single‐cell spatial transcriptomics enables comprehensive gene expression profiling with precise cellular localization within tissue architecture. To systematically evaluate the compatibility and performance of alternative sequencing platforms for this ...
Guangchuang Yu, Hongyuan Zhu
exaly   +2 more sources

The complete chloroplast genome of Artocarpus gongshanensis (Moraceae) [PDF]

Mitochondrial DNA. Part B. Resources
The chloroplast genome of Artocarpus gongshanensis S. K. Wu ex C. Y. Wu & S. S. Chang 1989, a threatened species endemic to China, was sequenced for the first time using Illumina NovaSeq 6000.
Aiping Yin, Yupin Fu, Qian Yang, Jiahua Li   +3 more
doaj   +2 more sources

HighALPS: ultra-high-throughput marker-gene amplicon library preparation and sequencing on the Illumina NextSeq and NovaSeq Platforms [PDF]

MSystems
Nicholas A Bokulich, Lena Flörl, Maria Domenica Moccia   +2 more
exaly   +2 more sources