Interdisciplinary MedicineSingle‐cell spatial transcriptomics enables comprehensive gene expression profiling with precise cellular localization within tissue architecture. To systematically evaluate the compatibility and performance of alternative sequencing platforms for this ...
Guangchuang Yu
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A recurrent sequencing artifact on Illumina sequencers with two-color fluorescent dye chemistry and its impact on somatic variant detection [PDF]
Genome BiologyBackground The sequencing-by-synthesis technology by Illumina, Inc. enables efficient and scalable readouts of mutations from genomic data. To enhance sequencing speed and efficiency, Illumina has shifted from the four-color base calling chemistry of the
Beverly J. Fu +4 more
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Surface sediments prokaryotic communities: five years of 16S rRNA amplicon sequencing data from the northernmost part of the Mediterranean SeaSRA - NCBI [PDF]
Data in BriefSurface sediments harbour diverse prokaryotic communities that play a key role in biogeochemical cycling and provide valuable insights when compared with water column communities, allowing for a more comprehensive understanding of marine ecosystem ...
Noemi Tomasi +3 more
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A large-scale comparative metagenomic analysis of short-read sequencing platforms indicates high taxonomic concordance and functional analysis challenge [PDF]
mSystemsDriven by the increasing scale of microbiome studies and the rise of large, continuously expanding population cohorts, the volume of sequencing data is growing rapidly.
Kinga Zielińska +4 more
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The complete mitochondrial genome of Myotis chinensis (Chiroptera: Vespertilionidae) and its phylogenetic analysis [PDF]
Mitochondrial DNA. Part B. ResourcesThe vesper bat, Myotis chinensis, is a member of the family Vespertilionidae and is found in Southeast Asia. There are, to date, no reports of the complete mitochondrial genome of this species.
Xinyu Miao, Lizhi Zhou
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Genome BiologyBackground Transcriptome sequencing (RNA-seq) is a powerful technology for gene expression profiling. Selection of optimal parameters for cDNA library generation is crucial for acquisition of high-quality data. In this study, we investigate the impact of
Natalia Zajac +2 more
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HighALPS: ultra-high-throughput marker-gene amplicon library preparation and sequencing on the Illumina NextSeq and NovaSeq Platforms [PDF]
MSystemsNicholas Bokulich, Lena Florl
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Scientific Data, 2023 Measurement(s) Whole Genome Sequencing • Chromosome assembly by Hi-C data • Whole Transcriptome Sequencing Technology Type(s) PacBio Sequel System • Hi-C • Illumina HiSeq. 2500 • Illumina NovaSeq.
Qingmin Zeng +6 more
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Scientific Data, 2022 Measurement(s) whole genome sequencing Technology Type(s) Illumina NovaSeq. 6000 • Oxford Nanopore Sequencing Sample Characteristic - Organism Phthorimaea operculella Sample Characteristic - Location Yunnan Province, Southwest ...
Mengdi Zhang +11 more
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Accuracy benchmark of the GeneMind GenoLab M sequencing platform for WGS and WES analysis
BMC Genomics, 2022 Background GenoLab M is a recently developed next-generation sequencing (NGS) platform from GeneMind Biosciences. To establish the performance of GenoLab M, we present the first report to benchmark and compare the WGS and WES sequencing data of the ...
Chaoyang Li +8 more
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