Results 21 to 30 of about 56,332 (214)

Isolating peripheral blood mononuclear cells from HIV-infected patients for single-cell RNA sequencing and integration analysis

STAR Protocols, 2023
Summary: Single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells (PBMCs) allows the dissection of transcriptional changes in immune cells with HIV infection. Here, we isolate PBMCs from HIV-infected individuals.
Jie Jia, Yu Zhao, Jian-Hua Wang, Yi-Qun Kuang   +3 more
doaj   +1 more source

Highly comparable metabarcoding results from MGI-Tech and Illumina sequencing platforms [PDF]

PeerJ, 2021
With the developments in DNA nanoball sequencing technologies and the emergence of new platforms, there is an increasing interest in their performance in comparison with the widely used sequencing-by-synthesis methods.
Sten Anslan, Vladimir Mikryukov, Kęstutis Armolaitis, Jelena Ankuda, Dagnija Lazdina, Kristaps Makovskis, Lars Vesterdal, Inger Kappel Schmidt, Leho Tedersoo   +8 more
doaj   +2 more sources

Development of de-novo transcriptome assembly and SSRs in allohexaploid Brassica with functional annotations and identification of heat-shock proteins for thermotolerance

Frontiers in Genetics, 2022
Crop Brassicas contain monogenomic and digenomic species, with no evidence of a trigenomic Brassica in nature. Through somatic fusion (Sinapis alba + B.
Kaushal Pratap Singh, Preetesh Kumari, Devendra Kumar Yadava   +2 more
doaj   +1 more source

The complete chloroplast genome of Pterobryopsis orientalis (Pterobryaceae, Hypnales) and its phylogenetic implications

Mitochondrial DNA. Part B. Resources, 2022
Pterobryopsis orientalis (Müll. Hal.) M. Fleisch. 1917, a subtropical moss from the family Pterobryaceae, is native to the western Himalaya. In this study, the complete chloroplast genome of P.
Wei Han, Ningning Yu, Yin Li, Li Zhang, Yu Jia   +4 more
doaj   +1 more source

Whole-Genome Survey and Microsatellite Marker Detection of Antarctic Crocodile Icefish, Chionobathyscus dewitti

Animals, 2022
The crocodile icefish, Chionobathyscus dewitti, belonging to the family Channichthyidae, is an endemic species of the Southern Ocean. The study of its biological features and genetics is challenging as the fish inhabits the deep sea around Antarctic ...
Jinmu Kim, Seung-Jae Lee, Euna Jo, Eunkyung Choi, Minjoo Cho, Soyun Choi, Jeong-Hoon Kim, Hyun Park   +7 more
doaj   +1 more source

The draft genome dataset of the American cockroach, Periplaneta americana (Linnaeus, 1758) (Blattidae: Blattinae)

Data in Brief, 2023
Periplaneta americana is a cosmopolitan pest cockroach endemic to tropical and subtropical climates. It occurs frequently in urban sewer and wastewater system and transit in human proximities, spreading pathogens that causes serious public health ...
Marcellinus Isaac Stia Dominic, Abdul Hafiz Ab Majid   +1 more
doaj   +1 more source

Transcriptome dataset to investigate the effect of deep seawater on the gene expression and functional compounds of leaves of Ligularia stenocephalaNCBI.

Data in Brief
Seawater is often applied to improve crop quality by supplying minerals to the crop. Due to high salt concentration of seawater, one of key factors for successful utilization of seawater in agriculture would be optimal dilution ratios, which can be ...
Dewi Komang Anggita, Bimpe Suliyat Azeez, Se-Jin Oh, Jong-Kuk Na   +3 more
doaj   +1 more source

The complete chloroplast genome of a medical herb, Potentilla parvifolia Fisch. (Rosaceae), from Qinghai-Tibet Plateau in China

Mitochondrial DNA. Part B. Resources, 2021
Potentilla parvifolia Fisch. (Rosaceae) is one of the genuine medicinal materials in Qinghai-Tibet Plateau, China. Here we report the first chloroplast (cp) genome of P. parvifolia using Illumina NovaSeq 6000 platform.
Jinping Li, Likuan Liu, Hongyu Wang, Caiming Li, Wenming Zuo, Yang Zeng   +5 more
doaj   +1 more source

An epithelial GPR35 isoform supports tumor‐associated transcriptional and metabolic phenotypes

FEBS Letters, EarlyView.
GPR35 generates two functionally distinct isoforms with previously unresolved roles. GPR35‐short mediates immune‐cell chemotaxis, while GPR35‐long is enriched in colorectal cancer epithelium, where it supports increased metabolism, proliferation, and tumor‐associated transcriptional programs.
Jørgen D. Rønneberg, Martine Hjeltnes, Josephine Krieger, Joshua E. Elias, Maria Stensland, Kristian Holm, Xiaojun Jiang, Chuki Khangsar, Tuula A. Nyman, Arthur Kaser, Johannes R. Hov, Tom H. Karlsen, Nicole C. Kaneider, Frode L. Jahnsen, Georg Schneditz   +14 more
wiley   +1 more source

Genomic dataset of a multiple-drug resistant Pseudomonas sp. strain RAC1 isolated from a flacherie infected Nistari race of Bombyx mori L.

Data in Brief
Species belonging to the genus Pseudomonas is a rod shaped Gram-negative bacteria emerged as an important silkworm pathogen with broad-level multi-drug resistance.
Rittick Mondal, Paulami Dam, Joydeep Chakraborty, Shubhajit Shaw, Sayantan Pradhan, Sandip Das, Jannatun Nesa, Khemraj Meena, Amit Ghati, Sandip Dev Chaudhuri, Debjoy Bhattacharjee, Vivekananda Mandal, Biraj Sarkar, Amit Kumar Mandal   +13 more
doaj   +1 more source